LOCUS       sequence-56436-e         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Baltz AG, Munschauer M, Schwanhausser B, Vasile A, Murakawa Y, 
            Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, 
            Bonneau R, Selbach M, Dieterich C, Landthaler M
  TITLE     The mRNA-Bound Proteome and Its Global Occupancy Profile on 
            Protein-Coding Transcripts.
  JOURNAL   Mol Cell. 2012 Jun 8;46(5):674-90.
  PUBMED    22681889
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2012-06-8"; volume: "46(5)"; pages: "674-90"
COMMENT     Sequence Label: pFRT/TO/HIS/FLAG/HA-HNRNPR
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    7..25
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    28..46
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     56..80
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             180..203
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             207..230
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             231..257
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    273..297
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gagctctccc tatcagtgat agagatctcc ctatcagtga tagagatcgt cgacgagctc
       61 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa
      121 gacaccggga ccgatccagc ctccggactc tagcgtttaa acttaagctt ggtaccatgc
      181 accaccacca ccaccaccac cacatggact acaaggacga cgatgacaag tacccttatg
      241 acgtgcccga ttacgctttg aaccagatat caacaagttt gtacaaaaaa gcaggctcca
      301 ccatggctaa tcaggtgaat ggtaatgcgg tacagttaaa agaagaggaa gaaccaatgg
      361 atacttccag tgtaactcac acagaacact acaagacact gatagaggca ggcctcccac
      421 agaaggtggc agaaagactt gatgaaatat ttcagacagg attggtagct tatgtcgatc
      481 ttgatgaaag agcaattgat gctctcaggg aatttaatga agaaggagct ctgtctgtac
      541 tacagcagtt caaggaaagt gacttatcac atgttcagaa caaaagtgca tttttatgtg
      601 gagttatgaa gacctacagg cagagagaga aacaggggag caaggtgcaa gagtccacaa
      661 agggacctga tgaagcgaag atcaaggcct tgcttgagag aactggttat actctggatg
      721 taaccacagg acagaggaag tatggtggtc ctccaccaga cagtgtgtac tctggcgtgc
      781 aacctggaat tggaacggag gtatttgtag gcaaaatacc aagggattta tatgaggatg
      841 agttggtgcc cctttttgag aa
//
