LOCUS       sequence-56451-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Longtine MS, McKenzie A 3rd, Demarini DJ, Shah NG, Wach A, Brachat 
            A, Philippsen P, Pringle JR
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 1998 Jul;14(10):953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1998-07"; volume: "14(10)"; pages: "953-61"
COMMENT     Sequence Label: pFA6a-TRP1-PGAL1
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(58..499)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(64..87)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    382..499
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     promoter        594..741
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
ORIGIN
        1 gctgcaggtc gacggatccc cgggttaatt aaagacattt tgagatccgg gttttttctc
       61 cttgacgtta aagtatagag gtatattaac aattttttgt tgatactttt attacatttg
      121 aataagaagt aatacaaacc gaaaatgttg aaagtattag ttaaagtggt tatgcagttt
      181 ttgcatttat atatctgtta atagatcaaa aatcatcgct tcgctgatta attaccccag
      241 aaataaggct aaaaaactaa tcgcattatc atcctatggt tgttaatttg attcgttcat
      301 ttgaaggttt gtggggccag gttactgcca atttttcctc ttcataacca taaaagctag
      361 tattgtagaa tctttattgt tcggagcagt gcggcgcgag gcacatctgc gtttcaggaa
      421 cgcgaccggt gaagacgagg acgcacggag gagagtcttc cttcggaggg ctgtcacccg
      481 ctcggcggct tctaatccgt acttcaatat agcaatgagc agttaagcgt attactgaaa
      541 gttccaaaga gaaggttttt ttaggctaag ataatggggc tctttacaga tctgtacaat
      601 cttgatccgg agcttttctt tttttgccga ttaagaatta attcggtcga aaaaagaaaa
      661 ggagagggcc aagagggagg gcattggtga ctattgagca cgtgagtata cgtgattaag
      721 cacacaaagg cagcttggag tatgtctgtt attaatttca caggtagttc tggtccattg
      781 gtgaaagttt gcggcttgca gagcacagag gccgcagaat gtgctctaga ttccgatgct
      841 gacttgctgg gtattatatg tgtgcccaat agaaagagaa caattgacc
//
