LOCUS       sequence-56456-e         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used for making transgenic mice. Contains a loxP-flanked cassette 
            with a PGK-neo selectable marker and a tpA transcriptional stop 
            sequence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Srinivas S, Watanabe T, Lin CS, William CM, Tanabe Y, Jessell TM, 
            Costantini F
  TITLE     Cre reporter strains produced by targeted insertion of EYFP and ECFP
            into the ROSA26 locus.
  JOURNAL   BMC Dev Biol. 2001;1:4. doi: 10.1186/1471-213x-1-4. Epub 2001 Mar 
            27.
  PUBMED    11299042
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Dev 
            Biol."; date: "2001"; volume: "1:4. doi"; pages: " 
            10.1186/1471-213x-1-4. Epub 2001 Mar 27"
COMMENT     Sequence Label: pBigT
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(3..68,69..106)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     primer_bind     139..155
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     139..155
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    169..202
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        232..731
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(267..290)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     664..683
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(808..827)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 tgtagggcgc agtagtccag ggtttccttg atgatgtcat acttatcctg tccctttttt
       61 ttccacagct cgcggttgag gacaaactct tcgcggtctt tccagtgggg atcgacggta
      121 tcgtagagtc gaggccgctc tagaactagt ggatccggaa cccttaatat aacttcgtat
      181 aatgtatgct atacgaagtt attaggtccc tcgacctgca ggaattctac cgggtagggg
      241 aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc
      301 gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg
      361 ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc
      421 cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta
      481 gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg
      541 gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg
      601 ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc
      661 cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc
      721 cgggcctttc gacctgcagc caatatggga tcggccattg aacaagatgg attgcacgca
      781 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
      841 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
      901 aagaccgacc tgtccggt
//
