LOCUS       sequence-56461-e         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Gokirmak T, Campanale JP, Shipp LE, Moy GW, Tao H, Hamdoun A
  TITLE     Localization and Substrate Selectivity of Sea Urchin Multidrug (MDR)
            Efflux Transporters.
  JOURNAL   J Biol Chem. 2012 Nov 2.
  PUBMED    23124201
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2012 Nov 2."
COMMENT     Sequence Label: pCS2+8CmOrange
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        51..69
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     51..68
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             155..865
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
ORIGIN
        1 tgctcgttta gggaaccgcc attctgcctg gggacgtcgg agcaagcttg atttaggtga
       61 cactatagaa tacaagctac ttgttctttt tgcaggatcc actagtggcg cgccattaat
      121 taaaggccgg ccagcgatcg ccggacatcg attgatggtg agcaagggcg aggagaataa
      181 catggccatc atcaaggagt tcatgcgctt caaggtgcgc atggagggct ccgtgaacgg
      241 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggct ttcagaccgc
      301 taagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
      361 tttcacctac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttcaa
      421 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aactacgagg acggcggcgt
      481 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
      541 gcgcggcacc aacttcccct ccgacggccc cgtgatgcag aagaagacca tgggctggga
      601 ggcctcctcc gagcggatgt accccgagga cggtgccctg aagggcaaga tcaagatgag
      661 gctgaagctg aaggacggcg gccactacac ctccgaggtc aagaccacct acaaggccaa
      721 gaagcccgtg cagctgcccg gcgcctacat cgtcgacatc aagttggaca tcacctccca
      781 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
      841 cggcatggac gagctgtaca agtaagaatt caaggcc
//
