LOCUS       sequence-56471-e         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Longtine MS, McKenzie A 3rd, Demarini DJ, Shah NG, Wach A, Brachat 
            A, Philippsen P, Pringle JR
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 1998 Jul;14(10):953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1998-07"; volume: "14(10)"; pages: "953-61"
COMMENT     Sequence Label: pFA6a-kanMX6-PGAL1-3HA
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(73..162)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     promoter        complement(203..644)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(209..232)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    527..644
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 cttatttaat aataaaaatc ataaatcata agaaattcgc ttatttagaa gtggcgcgcc
       61 tcagcactga gcagcgtaat ctggaacgtc atatggatag gatcctgcat agtccgggac
      121 gtcataggga tagcccgcat agtcaggaac atcgtatggg taaaagatgt taattaaaga
      181 cattttgaga tccgggtttt ttctccttga cgttaaagta tagaggtata ttaacaattt
      241 tttgttgata cttttattac atttgaataa gaagtaatac aaaccgaaaa tgttgaaagt
      301 attagttaaa gtggttatgc agtttttgca tttatatatc tgttaataga tcaaaaatca
      361 tcgcttcgct gattaattac cccagaaata aggctaaaaa actaatcgca ttatcatcct
      421 atggttgtta atttgattcg ttcatttgaa ggtttgtggg gccaggttac tgccaatttt
      481 tcctcttcat aaccataaaa gctagtattg tagaatcttt attgttcgga gcagtgcggc
      541 gcgaggcaca tctgcgtttc aggaacgcga ccggtgaaga cgaggacgca cggaggagag
      601 tcttccttcg gagggctgtc acccgctcgg cggcttctaa tccgtacttc aatatagcaa
      661 tgagcagtta agcgtattac tgaaagttcc aaagagaagg tttttttagg ctaagataat
      721 ggggctcttt acagatctgt ttagcttgcc tcgtccccgc cgggtcaccc ggccagcgac
      781 atggaggccc agaataccct ccttgacagt cttgacgtgc gcagctcagg ggcatgatgt
      841 gactgtcgcc cgtacattta gcccatacat ccccatgtat aatcatttgc atccatacat
      901 tttgatggcc gcacggcgcg aagcaaaaat tacggctcct cg
//
