LOCUS       sequence-56479-e         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Longtine MS, McKenzie A 3rd, Demarini DJ, Shah NG, Wach A, Brachat 
            A, Philippsen P, Pringle JR
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 1998 Jul;14(10):953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1998-07"; volume: "14(10)"; pages: "953-61"
COMMENT     Sequence Label: pFA6a-TRP1-PGAL1-3HA
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(72..161)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     promoter        complement(202..643)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(208..231)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    526..643
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     promoter        738..885
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
ORIGIN
        1 ttatttaata ataaaaatca taaatcataa gaaattcgct tatttagaag tggcgcgcct
       61 cagcactgag cagcgtaatc tggaacgtca tatggatagg atcctgcata gtccgggacg
      121 tcatagggat agcccgcata gtcaggaaca tcgtatgggt aaaagatgtt aattaaagac
      181 attttgagat ccgggttttt tctccttgac gttaaagtat agaggtatat taacaatttt
      241 ttgttgatac ttttattaca tttgaataag aagtaataca aaccgaaaat gttgaaagta
      301 ttagttaaag tggttatgca gtttttgcat ttatatatct gttaatagat caaaaatcat
      361 cgcttcgctg attaattacc ccagaaataa ggctaaaaaa ctaatcgcat tatcatccta
      421 tggttgttaa tttgattcgt tcatttgaag gtttgtgggg ccaggttact gccaattttt
      481 cctcttcata accataaaag ctagtattgt agaatcttta ttgttcggag cagtgcggcg
      541 cgaggcacat ctgcgtttca ggaacgcgac cggtgaagac gaggacgcac ggaggagagt
      601 cttccttcgg agggctgtca cccgctcggc ggcttctaat ccgtacttca atatagcaat
      661 gagcagttaa gcgtattact gaaagttcca aagagaaggt ttttttaggc taagataatg
      721 gggctcttta cagatctgta caatcttgat ccggagcttt tctttttttg ccgattaaga
      781 attaattcgg tcgaaaaaag aaaaggagag ggccaagagg gagggcattg gtgactattg
      841 agcacgtgag tatacgtgat taagcacaca aaggcagctt ggagtatgtc tg
//
