LOCUS       sequence-56481-e         979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Longtine MS, McKenzie A 3rd, Demarini DJ, Shah NG, Wach A, Brachat 
            A, Philippsen P, Pringle JR
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 1998 Jul;14(10):953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1998-07"; volume: "14(10)"; pages: "953-61"
COMMENT     Sequence Label: pFA6a-TRP1-PGAL1-3HA
FEATURES             Location/Qualifiers
     source          1..979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             53..61
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(141..815)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(816..963)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
ORIGIN
        1 cgcataggcc actagtggat ctgatatcat cgatgaattc gagctcgttt aaacctcctt
       61 acgcatctgt gcggtatttc acaccgcata gatcggcaag tgcacaaaca atacttaaat
      121 aaatactact cagtaataac ctatttctta gcatttttga cgaaatttgc tattttgtta
      181 gagtctttta caccatttgt ctccacacct ccgcttacat caacaccaat aacgccattt
      241 aatctaagcg catcaccaac attttctggc gtcagtccac cagctaacat aaaatgtaag
      301 ctttcggggc tctcttgcct tccaacccag tcagaaatcg agttccaatc caaaagttca
      361 cctgtcccac ctgcttctga atcaaacaag ggaataaacg aatgaggttt ctgtgaagct
      421 gcactgagta gtatgttgca gtcttttgga aatacgagtc ttttaataac tggcaaaccg
      481 aggaactctt ggtattcttg ccacgactca tctccatgca gttggacgat atcaatgccg
      541 taatcattga ccagagccaa aacatcctcc ttaggttgat tacgaaacac gccaaccaag
      601 tatttcggag tgcctgaact atttttatat gcttttacaa gacttgaaat tttccttgca
      661 ataaccgggt caattgttct ctttctattg ggcacacata taatacccag caagtcagca
      721 tcggaatcta gagcacattc tgcggcctct gtgctctgca agccgcaaac tttcaccaat
      781 ggaccagaac tacctgtgaa attaataaca gacatactcc aagctgcctt tgtgtgctta
      841 atcacgtata ctcacgtgct caatagtcac caatgccctc cctcttggcc ctctcctttt
      901 cttttttcga ccgaattaat tcttaatcgg caaaaaaaga aaagctccgg atcaagattg
      961 tacagatctg taaagagcc
//
