LOCUS       sequence-56483-e        1003 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Longtine MS, McKenzie A 3rd, Demarini DJ, Shah NG, Wach A, Brachat 
            A, Philippsen P, Pringle JR
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 1998 Jul;14(10):953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1998-07"; volume: "14(10)"; pages: "953-61"
COMMENT     Sequence Label: pFA6a-His3MX6-PGAL1-3HA
FEATURES             Location/Qualifiers
     source          1..1003
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(85..282)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(286..984)
                     /codon_start=1
                     /product="S. kluyveri imidazoleglycerol phosphate 
                     dehydratase"
                     /label=k-HIS3
                     /translation="MAEPAQKKQKQTVQERKAFISRITNETKIQIAISLNGGYIQIKDS
                     ILPAKKDDDVASQATQSQVIDIHTGVGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHT
                     TEDCGIALGQAFKEAMGAVRGVKRFGTGFAPLDEALSRAVVDLSSRPFAVIDLGLKREM
                     IGDLSTEMIPHFLESFAEAARITLHVDCLRGFNDHHRSESAFKALAVAIREAISSNGTN
                     DVPSTKGVLM"
ORIGIN
        1 cgcataggcc actagtggat ctgatatcat cgatgaattc gagctcgttt aaactggatg
       61 gcggcgttag tatcgaatcg acagcagtat agcgaccagc attcacatac gattgacgca
      121 tgatattact ttctgcgcac ttaacttcgc atctgggcag atgatgtcga ggcgaaaaaa
      181 aatataaatc acgctaacat ttgattaaaa tagaacaact acaatataaa aaaactatac
      241 aaatgacaag ttcttgaaaa caagaatctt tttattgtca gtacttcaca tcaaaacacc
      301 tttggttgag ggaacgtcat tggtgccatt gctagaaata gcttctctta tggcaacagc
      361 caaagccttg aacgcactct cacttctgtg gtgatcgttg aaacctctca gacaatcaac
      421 atgcaaagta attctggccg cctccgcgaa actttccaaa aagtgtggaa tcatttcagt
      481 ggataaatca ccaatcatct ctctcttcaa tccaaggtcg attacagcaa atggtctact
      541 agataaatcg actacggcac gtgatagcgc ctcatccaat ggtgcgaacc cagtaccgaa
      601 tctttttaca ccacggacag cacccattgc ttctttgaac gcttgcccta atgcgatacc
      661 gcaatcttcg gtagtatggt gatcgtcaat gtgcaggtca ccaatacatt caacaataag
      721 agaccaacca gagtgttttg ccaacgcatg gatcatatga tccaaaaagc caacacctgt
      781 gtgaatatcg atgacctgtg actgagtagc ttgggaagct acatcgtcat ccttctttgc
      841 aggaagaatc gaatctttta tttgaatata accaccattc agcgaaatag cgatttgaat
      901 tttagtttca ttagtgatac gggagataaa cgccttgcgc tcctgaacag tttgtttttg
      961 ctttttttgg gctggttctg ccatggttgt ttatgttcgg atg
//
