LOCUS       sequence-56514-e       10528 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10528)
  AUTHORS   Gurtan AM, Lu V, Bhutkar A, Sharp PA
  TITLE     In vivo structure-function analysis of human Dicer reveals 
            directional processing of precursor miRNAs.
  JOURNAL   RNA. 2012 Jun;18(6):1116-22. Epub 2012 Apr 30.
  PUBMED    22546613
REFERENCE   2  (bases 1 to 10528)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA."; 
            date: "2012-06"; volume: "18(6)"; pages: "1116-22. Epub 2012 Apr 30"
COMMENT     Sequence Label: pCAGGS-Flag-hsDicer (Y971A/Y972A)
FEATURES             Location/Qualifiers
     source          1..10528
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..661
                     /label=chicken beta-actin promoter
     intron          662..1674
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1598..1620
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1682..1701
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1748..1771
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(7550..7569)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7615..7670
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7669..7688)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8031..8047)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8031..8047)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8044..8066)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8055..8071
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8079..8085,8086..8103,8104..8109))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8124..8145
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8204..8399
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8250..8385
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    8405..8539
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8442..8461)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8496..8515
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8607..8624)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8778..9366)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8858..8877)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9537..10328,10329..10397))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10160..10179
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10398..10502)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc
     1321 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
     1381 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
     1441 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     1501 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
     1561 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     1621 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     1681 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattctc gagattgatc
     1741 taccatggac tacaaagacg atgacgacaa gcttaaaagc cctgctttgc aacccctcag
     1801 catggcaggc ctgcagctca tgacccctgc ttcctcacca atgggtcctt tctttggact
     1861 gccatggcaa caagaagcaa ttcatgataa catttatacg ccaagaaaat atcaggttga
     1921 actgcttgaa gcagctctgg atcataatac catcgtctgt ttaaacactg gctcagggaa
     1981 gacatttatt gcagtactac tcactaaaga gctgtcctat cagatcaggg gagacttcag
     2041 cagaaatgga aaaaggacgg tgttcttggt caactctgca aaccaggttg ctcaacaagt
     2101 gtcagctgtc agaactcatt cagatctcaa ggttggggaa tactcaaacc tagaagtaaa
     2161 tgcatcttgg acaaaagaga gatggaacca agagtttact aagcaccagg ttctcattat
     2221 gacttgctat gtcgccttga atgttttgaa aaatggttac ttatcactgt cagacattaa
     2281 ccttttggtg tttgatgagt gtcatcttgc aatcctagac cacccctatc gagaaattat
     2341 gaagctctgt gaaaattgtc catcatgtcc tcgcattttg ggactaactg cttccatttt
     2401 aaatgggaaa tgtgatccag aggaattgga agaaaagatt cagaaactag agaaaattct
     2461 taagagtaat gctgaaactg caactgacct ggtggtctta gacaggtata cttctcagcc
     2521 atgtgagatt gtggtggatt gtggaccatt tactgacaga agtgggcttt atgaaagact
     2581 gctgatggaa ttagaagaag cacttaattt tatcaatgat tgtaatatat ctgtacattc
     2641 aaaagaaaga gattctactt taatttcgaa acagatacta tcagactgtc gtgccgtatt
     2701 ggtagttctg ggaccctggt gtgcagataa agtagctgga atgatggtaa gagaactaca
     2761 gaaatacatc aaacatgagc aagaggagct gcacaggaaa tttttattgt ttacagacac
     2821 tttcctaagg aaaatacatg cactatgtga agagcacttc tcacctgcct cacttgacct
     2881 gaaatttgta actcctaaag taatcaaact gctcgaaatc ttacgcaaat ataaaccata
     2941 tgagcgacag cagtttgaaa gcgttgagtg gtataataat agaaatcagg ataattatgt
     3001 gtcatggagt gattctgagg atgatgatga ggatgaagaa attgaagaaa aagagaagcc
     3061 agagacaaat tttccttctc cttttaccaa cattttgtgc ggaattattt ttgtggaaag
     3121 aagatacaca gcagttgtct taaacagatt gataaaggaa gctggcaaac aagatccaga
     3181 gctggcttat atcagtagca atttcataac tggacatggc attgggaaga atcagcctcg
     3241 caacaaacag atggaagcag aattcagaaa acaggaagag gtacttagga aatttcgagc
     3301 acatgagacc aacctgctta ttgcaacaag tattgtagaa gagggtgttg atataccaaa
     3361 atgcaacttg gtggttcgtt ttgatttgcc cacagaatat cgatcctatg ttcaatctaa
     3421 aggaagagca agggcaccca tctctaatta tataatgtta gcggatacag acaaaataaa
     3481 aagttttgaa gaagacctta aaacctacaa agctattgaa aagatcttga gaaacaagtg
     3541 ttccaagtcg gttgatactg gtgagactga cattgatcct gtcatggatg atgatgacgt
     3601 tttcccacca tatgtgttga ggcctgacga tggtggtcca cgagtcacaa tcaacacggc
     3661 cattggacac atcaatagat actgtgctag attaccaagt gatccgttta ctcatctagc
     3721 tcctaaatgc agaacccgag agttgcctga tggtacattt tattcaactc tttatctgcc
     3781 aattaactca cctcttcgag cctccattgt tggtccacca atgagctgtg tacgattggc
     3841 tgaaagagtt gtagctctca tttgctgtga gaaactgcac aaaattggcg aactggatga
     3901 ccatttgatg ccagttggga aagagactgt taaatatgaa gaggagcttg atttgcatga
     3961 tgaagaagag accagtgttc caggaagacc aggttccacg aaacgaaggc agtgctaccc
     4021 aaaagcaatt ccagagtgtt tgagggatag ttatcccaga cctgatcagc cctgttacct
     4081 gtatgtgata ggaatggttt taactacacc tttacctgat gaactcaact ttagaaggcg
     4141 gaagctctat cctcctgaag ataccacaag atgctttgga atactgacgg ccaaacccat
     4201 acctcagatt ccacactttc ctgtgtacac acgctctgga gaggttacca tatccattga
     4261 gttgaagaag tctggtttca tgttgtctct acaaatgctt gagttgatta caagacttca
     4321 ccagtatata ttctcacata ttcttcggct tgaaaaacct gcactagaat ttaaacctac
     4381 agacgctgat tcagcatact gtgttctacc tcttaatgtt gttaatgact ccagcacttt
     4441 ggatattgac tttaaattca tggaagatat tgagaagtct gaagctcgca taggcattcc
     4501 cagtacaaag tatacaaaag aaacaccctt tgtttttaaa ttagaagatt accaagatgc
     4561 cgttatcatt ccaagatatc gcaattttga tcagcctcat cgattttatg tagctgatgt
     4621 gtacactgat cttaccccac tcagtaaatt tccttcccct gagtatgaaa cttttgcaga
     4681 agctgctaaa acaaagtaca accttgacct aaccaatctc aaccagccac tgctggatgt
     4741 ggaccacaca tcttcaagac ttaatctttt gacacctcga catttgaatc agaaggggaa
     4801 agcgcttcct ttaagcagtg ctgagaagag gaaagccaaa tgggaaagtc tgcagaataa
     4861 acagatactg gttccagaac tctgtgctat acatccaatt ccagcatcac tgtggagaaa
     4921 agctgtttgt ctccccagca tactttatcg ccttcactgc cttttgactg cagaggagct
     4981 aagagcccag actgccagcg atgctggcgt gggagtcaga tcacttcctg cggattttag
     5041 ataccctaac ttagacttcg ggtggaaaaa atctattgac agcaaatctt tcatctcaat
     5101 ttctaactcc tcttcagctg aaaatgataa ttactgtaag cacagcacaa ttgtccctga
     5161 aaatgctgca catcaaggtg ctaatagaac ctcctctcta gaaaatcatg accaaatgtc
     5221 tgtgaactgc agaacgttgc tcagcgagtc ccctggtaag ctccacgttg aagtttcagc
     5281 agatcttaca gcaattaatg gtctttctta caatcaaaat ctcgccaatg gcagttatga
     5341 tttagctaac agagactttt gccaaggaaa tcagctaaat tactacaagc aggaaatacc
     5401 cgtgcaacca actacctcat attccattca gaatttatac agttacgaga accagcccca
     5461 gcccagcgat gaatgtactc tcctgagtaa taaatacctt gatggaaatg ctaacaaatc
     5521 tacctcagat ggaagtcctg tgatggccgt aatgcctggt acgacagaca ctattcaagt
     5581 gctcaagggc aggatggatt ctgagcagag cccttctatt gggtactcct caaggactct
     5641 tggccccaat cctggactta ttcttcaggc tttgactctg tcaaacgcta gtgatggatt
     5701 taacctggag cggcttgaaa tgcttggcga ctccttttta aagcatgcca tcaccacata
     5761 tctattttgc acttaccctg atgcgcatga gggccgcctt tcatatatga gaagcaaaaa
     5821 ggtcagcaac tgtaatctgt atcgccttgg aaaaaagaag ggactaccca gccgcatggt
     5881 ggtgtcaata tttgatcccc ctgtgaattg gcttcctcct ggttatgtag taaatcaaga
     5941 caaaagcaac acagataaat gggaaaaaga tgaaatgaca aaagactgca tgctggcgaa
     6001 tggcaaactg gatgaggatt acgaggagga ggatgaggag gaggagagcc tgatgtggag
     6061 ggctccgaag gaagaggctg actatgaaga tgatttcctg gagtatgatc aggaacatat
     6121 cagatttata gataatatgt taatggggtc aggagctttt gtaaagaaaa tctctctttc
     6181 tcctttttca accactgatt ctgcatatga atggaaaatg cccaaaaaat cctccttagg
     6241 tagtatgcca ttttcatcag attttgagga ttttgactac agctcttggg atgcaatgtg
     6301 ctatctggat cctagcaaag ctgttgaaga agatgacttt gtggtggggt tctggaatcc
     6361 atcagaagaa aactgtggtg ttgacacggg aaagcagtcc atttcttacg acttgcacac
     6421 tgagcagtgt attgctgaca aaagcatagc ggactgtgtg gaagccctgc tgggctgcta
     6481 tttaaccagc tgtggggaga gggctgctca gcttttcctc tgttcactgg ggctgaaggt
     6541 gctcccggta attaaaagga ctgatcggga aaaggccctg tgccctactc gggagaattt
     6601 caacagccaa caaaagaacc tttcagtgag ctgtgctgct gcttctgtgg ccagttcacg
     6661 ctcttctgta ttgaaagact cggaatatgg ttgtttgaag attccaccaa gatgtatgtt
     6721 tgatcatcca gatgcagata aaacactgaa tcaccttata tcggggtttg aaaattttga
     6781 aaagaaaatc aactacagat tcaagaataa ggcttacctt ctccaggctt ttacacatgc
     6841 ctcctaccac tacaatacta tcactgattg ttaccagcgc ttagaattcc tgggagatgc
     6901 gattttggac tacctcataa ccaagcacct ttatgaagac ccgcggcagc actccccggg
     6961 ggtcctgaca gacctgcggt ctgccctggt caacaacacc atctttgcat cgctggctgt
     7021 aaagtacgac taccacaagt acttcaaagc tgtctctcct gagctcttcc atgtcattga
     7081 tgactttgtg cagtttcagc ttgagaagaa tgaaatgcaa ggaatggatt ctgagcttag
     7141 gagatctgag gaggatgaag agaaagaaga ggatattgaa gttccaaagg ccatggggga
     7201 tatttttgag tcgcttgctg gtgccattta catggatagt gggatgtcac tggagacagt
     7261 ctggcaggtg tactatccca tgatgcggcc actaatagaa aagttttctg caaatgtacc
     7321 ccgttcccct gtgcgagaat tgcttgaaat ggaaccagaa actgccaaat ttagcccggc
     7381 tgagagaact tacgacggga aggtcagagt cactgtggaa gtagtaggaa aggggaaatt
     7441 taaaggtgtt ggtcgaagtt acaggattgc caaatctgca gcagcaagaa gagccctccg
     7501 aagcctcaaa gctaatcaac ctcaggttcc caatagctga ggtaccagat ctttttccct
     7561 ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa
     7621 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga
     7681 catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa
     7741 catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata
     7801 tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga
     7861 ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac
     7921 atgttttact agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc
     7981 ttctcttatg gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg
     8041 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     8101 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     8161 ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga tccgcatctc aattagtcag
     8221 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     8281 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     8341 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     8401 agctaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     8461 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     8521 atcttatcat gtctggatcc gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     8581 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     8641 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     8701 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     8761 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     8821 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     8881 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     8941 tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg
     9001 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     9061 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     9121 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     9181 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     9241 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     9301 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     9361 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     9421 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     9481 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     9541 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     9601 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     9661 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     9721 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     9781 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     9841 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     9901 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     9961 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
    10021 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
    10081 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
    10141 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
    10201 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
    10261 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
    10321 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
    10381 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    10441 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    10501 cgcacatttc cccgaaaagt gccacctg
//
