LOCUS       sequence-56519-e         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Longtine MS, McKenzie A 3rd, Demarini DJ, Shah NG, Wach A, Brachat 
            A, Philippsen P, Pringle JR
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 1998 Jul;14(10):953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1998-07"; volume: "14(10)"; pages: "953-61"
COMMENT     Sequence Label: pFA6a-TRP1-PGAL1-GST
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(45..698)
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     complement(65..87)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     primer_bind     complement(739..762)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 caggtcgacg gatctggcgc gcctcaacgc ggaaccagat ccgattttgg aggatggtcg
       61 ccaccaccaa acgtggcttg ccagccctgc aaaggccatg ctatatactt gctggatttc
      121 aagtacttat caatttgtgg gatagcttca atacgttttt taaaacaaac taattttggg
      181 aacgcatcca ggcacattgg gtccatgtat aaaacaacat caagagcgtc atacaacatg
      241 aagtcaggat gggttacatg atcaccattt aaatatgttt tatgacataa acgatcttcg
      301 aacattttca gcatttcagg tagcttgcta agaaaatcaa ctttgagagt ttcaaagtct
      361 ttactatatg caattctcga aacaccgtat ctaatatcca aaaccgctcc ttcaagcatt
      421 gaaatctctg cacgctcttt tggacaacca cccaacatgt tgtgcttgtc agctatataa
      481 cgtatgatgg ccatagactg tgttaattta acatcaccat caatataata aggaagattg
      541 ggaaactcca aacccaattc aaactttttg tttcgccatt tatcaccttc atcgcgctca
      601 tacaaatgct cttcatattt ttcttcaaga tattccaaaa gaagtcgagt gggttgcaca
      661 aggcccttaa ttttccaata acctagtata ggggacatat taattaaaga cattttgaga
      721 tccgggtttt ttctccttga cgttaaagta tagaggtata ttaacaattt tttgttgata
      781 cttttattac atttgaataa gaagtaatac aaaccgaaaa tgttgaaagt attagttaaa
      841 gtggttatgc agtttttgca tttatatatc tgttaataga tcaaaaatca tcgcttcgct
      901 gattaattac cccagaaata aggctaaaa
//
