LOCUS       sequence-56522-e         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Longtine MS, McKenzie A 3rd, Demarini DJ, Shah NG, Wach A, Brachat 
            A, Philippsen P, Pringle JR
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 1998 Jul;14(10):953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1998-07"; volume: "14(10)"; pages: "953-61"
COMMENT     Sequence Label: pFA6a-His3MX6-PGAL1-GST
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(48..701)
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     complement(68..90)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     primer_bind     complement(742..765)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 ctgcaggtcg acggatctgg cgcgcctcaa cgcggaacca gatccgattt tggaggatgg
       61 tcgccaccac caaacgtggc ttgccagccc tgcaaaggcc atgctatata cttgctggat
      121 ttcaagtact tatcaatttg tgggatagct tcaatacgtt ttttaaaaca aactaatttt
      181 gggaacgcat ccaggcacat tgggtccatg tataaaacaa catcaagagc gtcatacaac
      241 atgaagtcag gatgggttac atgatcacca tttaaatatg ttttatgaca taaacgatct
      301 tcgaacattt tcagcatttc aggtagcttg ctaagaaaat caactttgag agtttcaaag
      361 tctttactat atgcaattct cgaaacaccg tatctaatat ccaaaaccgc tccttcaagc
      421 attgaaatct ctgcacgctc ttttggacaa ccacccaaca tgttgtgctt gtcagctata
      481 taacgtatga tggccataga ctgtgttaat ttaacatcac catcaatata ataaggaaga
      541 ttgggaaact ccaaacccaa ttcaaacttt ttgtttcgcc atttatcacc ttcatcgcgc
      601 tcatacaaat gctcttcata tttttcttca agatattcca aaagaagtcg agtgggttgc
      661 acaaggccct taattttcca ataacctagt ataggggaca tattaattaa agacattttg
      721 agatccgggt tttttctcct tgacgttaaa gtatagaggt atattaacaa ttttttgttg
      781 atacttttat tacatttgaa taagaagtaa tacaaaccga aaatgttgaa agtattagtt
      841 aaagtggtta tgcagttttt gcatttatat atctgttaat agatcaaaaa tcatcgcttc
      901 gctgattaat taccccagaa ataaggcta
//
