LOCUS       sequence-56525-e         956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Longtine MS, McKenzie A 3rd, Demarini DJ, Shah NG, Wach A, Brachat 
            A, Philippsen P, Pringle JR
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 1998 Jul;14(10):953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1998-07"; volume: "14(10)"; pages: "953-61"
COMMENT     Sequence Label: pFA6a-His3MX6-PGAL1-GST
FEATURES             Location/Qualifiers
     source          1..956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(56..497)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(62..85)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    380..497
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 ataacctagt ataggggaca tattaattaa agacattttg agatccgggt tttttctcct
       61 tgacgttaaa gtatagaggt atattaacaa ttttttgttg atacttttat tacatttgaa
      121 taagaagtaa tacaaaccga aaatgttgaa agtattagtt aaagtggtta tgcagttttt
      181 gcatttatat atctgttaat agatcaaaaa tcatcgcttc gctgattaat taccccagaa
      241 ataaggctaa aaaactaatc gcattatcat cctatggttg ttaatttgat tcgttcattt
      301 gaaggtttgt ggggccaggt tactgccaat ttttcctctt cataaccata aaagctagta
      361 ttgtagaatc tttattgttc ggagcagtgc ggcgcgaggc acatctgcgt ttcaggaacg
      421 cgaccggtga agacgaggac gcacggagga gagtcttcct tcggagggct gtcacccgct
      481 cggcggcttc taatccgtac ttcaatatag caatgagcag ttaagcgtat tactgaaagt
      541 tccaaagaga aggttttttt aggctaagat aatggggctc tttacagatc tgtttagctt
      601 gcctcgtccc cgccgggtca cccggccagc gacatggagg cccagaatac cctccttgac
      661 agtcttgacg tgcgcagctc aggggcatga tgtgactgtc gcccgtacat ttagcccata
      721 catccccatg tataatcatt tgcatccata cattttgatg gccgcacggc gcgaagcaaa
      781 aattacggct cctcgctgca gacctgcgag cagggaaacg ctcccctcac agacgcgttg
      841 aattgtcccc acgccgcgcc cctgtagaga aatataaaag gttaggattt gccactgagg
      901 ttcttctttc atatacttcc ttttaaaatc ttgctaggat acagttctca catcac
//
