LOCUS       sequence-56529-e         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Longtine MS, McKenzie A 3rd, Demarini DJ, Shah NG, Wach A, Brachat 
            A, Philippsen P, Pringle JR
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 1998 Jul;14(10):953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1998-07"; volume: "14(10)"; pages: "953-61"
COMMENT     Sequence Label: pFA6a-kanMX6-PGAL1-GFP
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(54..495)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(60..83)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    378..495
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 aaagttcttc tcctttactg ttaattaaag acattttgag atccgggttt tttctccttg
       61 acgttaaagt atagaggtat attaacaatt ttttgttgat acttttatta catttgaata
      121 agaagtaata caaaccgaaa atgttgaaag tattagttaa agtggttatg cagtttttgc
      181 atttatatat ctgttaatag atcaaaaatc atcgcttcgc tgattaatta ccccagaaat
      241 aaggctaaaa aactaatcgc attatcatcc tatggttgtt aatttgattc gttcatttga
      301 aggtttgtgg ggccaggtta ctgccaattt ttcctcttca taaccataaa agctagtatt
      361 gtagaatctt tattgttcgg agcagtgcgg cgcgaggcac atctgcgttt caggaacgcg
      421 accggtgaag acgaggacgc acggaggaga gtcttccttc ggagggctgt cacccgctcg
      481 gcggcttcta atccgtactt caatatagca atgagcagtt aagcgtatta ctgaaagttc
      541 caaagagaag gtttttttag gctaagataa tggggctctt tacagatctg tttagcttgc
      601 ctcgtccccg ccgggtcacc cggccagcga catggaggcc cagaataccc tccttgacag
      661 tcttgacgtg cgcagctcag gggcatgatg tgactgtcgc ccgtacattt agcccataca
      721 tccccatgta taatcatttg catccataca ttttgatggc cgcacggcgc gaagcaaaaa
      781 ttacggctcc tcgctgcaga cctgcgagca gggaaacgct cccctcacag acgcgttgaa
      841 ttgtccccac gccgcgcccc tgtagagaaa tataaaaggt taggatttgc cactgaggtt
      901 cttctttcat atacttcctt ttaaaatctt gctaggatac agttctcaca tcacatccga
      961 acataaaca
//
