LOCUS       sequence-56533-e        1028 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Longtine MS, McKenzie A 3rd, Demarini DJ, Shah NG, Wach A, Brachat 
            A, Philippsen P, Pringle JR
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 1998 Jul;14(10):953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1998-07"; volume: "14(10)"; pages: "953-61"
COMMENT     Sequence Label: pFA6a-His3MX6-PGAL1-GFP
FEATURES             Location/Qualifiers
     source          1..1028
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(49..236)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     157..181
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(260..970)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(300..320)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     915..943
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gctgcaggtc gacggatcta tattaccctg ttatccctag cggatctgcc ggtagaggtg
       61 tggtcaataa gagcgacctc atactatacc tgagaaagca acctgaccta caggaaagag
      121 ttactcaaga ataagaattt tcgttttaaa acctaagagt cactttaaaa tttgtataca
      181 cttatttttt ttataactta tttaataata aaaatcataa atcataagaa attcgcttat
      241 ttagaagtgg cgcgccctat ttgtatagtt catccatgcc atgtgtaatc ccagcagctg
      301 ttacaaactc aagaaggacc atgtggtctc tcttttcgtt gggatctttc gaaagggcag
      361 attgtgtgga caggtaatgg ttgtctggta aaaggacagg gccatcgcca attggagtat
      421 tttgttgata atggtctgct agttgaacgc ttccatcttc aatgttgtgt ctaattttga
      481 agttaacttt gattccattc ttttgtttgt ctgccatgat gtatacattg tgtgagttat
      541 agttgtattc caatttgtgt ccaagaatgt ttccatcttc tttaaaatca atacctttta
      601 actcgattct attaacaagg gtatcacctt caaacttgac ttcagcacgt gtcttgtagt
      661 tcccgtcatc tttgaaaaat atagttcttt cctgtacata accttcgggc atggcactct
      721 tgaaaaagtc atgccgtttc atatgatctg ggtatcttga aaagcattga acaccataag
      781 tgaaagtagt gacaagtgtt ggccatggaa caggtagttt tccagtagtg caaataaatt
      841 taagggtaag ttttccgtat gttgcatcac cttcaccctc tccactgaca gaaaatttgt
      901 gcccattaac atcaccatct aattcaacaa gaattgggac aactccagtg aaaagttctt
      961 ctcctttact gttaattaaa gacattttga gatccgggtt ttttctcctt gacgttaaag
     1021 tatagagg
//
