LOCUS       sequence-56535-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Longtine MS, McKenzie A 3rd, Demarini DJ, Shah NG, Wach A, Brachat 
            A, Philippsen P, Pringle JR
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 1998 Jul;14(10):953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1998-07"; volume: "14(10)"; pages: "953-61"
COMMENT     Sequence Label: pFA6a-His3MX6-PGAL1-GFP
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(48..489)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(54..77)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    372..489
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 cttctccttt actgttaatt aaagacattt tgagatccgg gttttttctc cttgacgtta
       61 aagtatagag gtatattaac aattttttgt tgatactttt attacatttg aataagaagt
      121 aatacaaacc gaaaatgttg aaagtattag ttaaagtggt tatgcagttt ttgcatttat
      181 atatctgtta atagatcaaa aatcatcgct tcgctgatta attaccccag aaataaggct
      241 aaaaaactaa tcgcattatc atcctatggt tgttaatttg attcgttcat ttgaaggttt
      301 gtggggccag gttactgcca atttttcctc ttcataacca taaaagctag tattgtagaa
      361 tctttattgt tcggagcagt gcggcgcgag gcacatctgc gtttcaggaa cgcgaccggt
      421 gaagacgagg acgcacggag gagagtcttc cttcggaggg ctgtcacccg ctcggcggct
      481 tctaatccgt acttcaatat agcaatgagc agttaagcgt attactgaaa gttccaaaga
      541 gaaggttttt ttaggctaag ataatggggc tctttacaga tctgtttagc ttgcctcgtc
      601 cccgccgggt cacccggcca gcgacatgga ggcccagaat accctccttg acagtcttga
      661 cgtgcgcagc tcaggggcat gatgtgactg tcgcccgtac atttagccca tacatcccca
      721 tgtataatca tttgcatcca tacattttga tggccgcacg gcgcgaagca aaaattacgg
      781 ctcctcgctg cagacctgcg agcagggaaa cgctcccctc acagacgcgt tgaattgtcc
      841 ccacgccgcg cccctgtaga gaaatataaa aggttaggat ttgccactga ggttcttctt
//
