LOCUS       sequence-56574-e         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  TITLE     SGC SIRT6 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: SIRT6
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             55..72
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             82..99
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tcctctagaa atattttgtt tactttaaga aggagatata ccatgggcag cagccatcat
       61 catcatcatc acagcagcgg cctggttccg cgtggtagtg tgaattacgc ggcggggctg
      121 tcgccgtacg cggacaaggg caagtgcggc ctcccggaga tcttcgaccc cccggaggag
      181 ctggagcgga aggtgtggga actggcgagg ctggtctggc agtcttccag tgtggtgttc
      241 cacacgggtg ccggcatcag cactgcctct ggcatccccg acttcagggg tccccacgga
      301 gtctggacca tggaggagcg aggtctggcc cccaagttcg acaccacctt tgagagcgcg
      361 cggcccacgc agacccacat ggcgctggtg cagctggagc gcgtgggcct cctccgcttc
      421 ctggtcagcc agaacgtgga cgggctccat gtgcgctcag gcttccccag ggacaaactg
      481 gcagagctcc acgggaacat gtttgtggaa gaatgtgcca agtgtaagac gcagtacgtc
      541 cgagacacag tcgtgggcac catgggcctg aaggccacgg gccggctctg caccgtggct
      601 aaggcaaggg ggctgcgagc ctgcagggga gagctgaggg acaccatcct agactgggag
      661 gactccctgc ccgaccggga cctggcactc gccgatgagg ccagcaggaa cgccgacctg
      721 tccatcacgc tgggtacatc gctgcagatc cggcccagcg ggaacctgcc gctggctacc
      781 aagcgccggg gaggccgcct ggtcatcgtc aacctgcagc ccaccaagca cgaccgccat
      841 gctgacctcc gcatccatgg ctacgttgac gaggtcatga cccggctcat gaagcacctg
      901 gggctggaga tccccgcctg ggacggccc
//
