LOCUS       sequence-56577-e         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  TITLE     SGC SIRT6 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: SIRT6
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             57..74
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             84..101
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ccctctagaa ataattttgt ttaactttaa gaaggagata taccatgggc agcagccatc
       61 accatcatca tcacagcagc ggcctggttc cgcgtggtag tgtgaattac gcggcggggc
      121 tgtcgccgta cgcggacaag ggcaagtgcg gcctcccgga gatcttcgac cccccggagg
      181 agctggagcg gaaggtgtgg gaactggcga ggctggtctg gcagtcttcc agtgtggtgt
      241 tccacacggg tgccggcatc agcactgcct ctggcatccc cgacttcagg ggtccccacg
      301 gagtctggac catggaggag cgaggtctgg cccccaagtt cgacaccacc tttgagagcg
      361 cgcggcccac gcagacccac atggcgctgg tgcagctgga gcgcgtgggc ctcctccgct
      421 tcctggtcag ccagaacgtg gacgggctcc atgtgcgctc aggcttcccc agggacaaac
      481 tggcagagct ccacgggaac atgtttgtgg aagaatgtgc caagtgtaag acgcagtacg
      541 tccgagacac agtcgtgggc accatgggcc tgaaggccac gggccggctc tgcaccgtgg
      601 ctaaggcaag ggggctgcga gcctgcaggg gagagctgag ggacaccatc ctagactggg
      661 aggactccct gcccgaccgg gacctggcac tcgccgatga ggccagcagg aacgccgacc
      721 tgtccatcac gctgggtaca tcgctgcaga tccggcccag cgggaacctg ccgctggcta
      781 ccaagcgccg gggaggccgc ctggtcatcg tcaacctgca gcccaccaag cacgaccgcc
      841 atgctgacct ccgcatccat ggctacgttg acgaggtcat gacccggctc atg
//
