LOCUS       sequence-56638-e         725 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  destination vector for the Golden Gate TALEN kit, directs expression
            of TALENs from a truncated CAGs promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Carlson DF, Tan W, Lillico SG, Stverakova D, Proudfoot C, Christian 
            M, Voytas DF, Long CR, Whitelaw CB, Fahrenkrug SC
  TITLE     Efficient TALEN-mediated gene knockout in livestock.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Oct 1.
  PUBMED    23027955
REFERENCE   2  (bases 1 to 725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2012 Oct 1."
COMMENT     Sequence Label: pC-GoldyTALEN
FEATURES             Location/Qualifiers
     source          1..725
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             74..94
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             95..121
                     /codon_start=1
                     /product="epitope tag from the baculovirus Autographa 
                     californica GP64 envelope fusion protein"
                     /label=AcV5 tag
                     /translation="SWKDASGWS"
     primer_bind     682..704
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     696..713
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     697..713
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 agctaggatc taactcgaga aagatattgt atatatcgta acaataggag gttcaacaat
       61 ggcttcctcc cctccaaaga aaaagagaaa ggttagttgg aaggacgcaa gtggttggtc
      121 tagagtggat ctacgcacgc tcggctacag tcagcagcag caagagaaga tcaaaccgaa
      181 ggtgcgttcg acagtggcgc agcaccacga ggcactggtg ggccatgggt ttacacacgc
      241 gcacatcgtt gcgctcagcc aacacccggc agcgttaggg accgtcgctg tcacgtatca
      301 gcacataatc acggcgttgc cagaggcgac acacgaagac atcgttggcg tcggcaaaca
      361 gtggtccggc gcacgcgccc tggaggcctt gctcacggat gcgggggagt tgagaggtcc
      421 gccgttacag ttggacacag gccaacttgt gaagattgca aaacgtggcg gcgtgaccgc
      481 aatggaggca gtgcatgcat cgcgcaatgc actgacgggt gcccccctgg agacgggcgc
      541 cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
      601 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
      661 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg
      721 cgtaa
//
