LOCUS       sequence-56639-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  destination vector for the Golden Gate TALEN kit, directs expression
            of TALENs from a truncated CAGs promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Carlson DF, Tan W, Lillico SG, Stverakova D, Proudfoot C, Christian 
            M, Voytas DF, Long CR, Whitelaw CB, Fahrenkrug SC
  TITLE     Efficient TALEN-mediated gene knockout in livestock.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Oct 1.
  PUBMED    23027955
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2012 Oct 1."
COMMENT     Sequence Label: pC-GoldyTALEN
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(16..36)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(16..34)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(55..71)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(55..71)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(68..90)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    79..95
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(103..109,110..127,128..133))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    148..169
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ctccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata
       61 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
      121 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
      181 ctcactgccc gctttccacc ggtcgtctcc aacgaccacc tcgtcgcctt ggcctgcctc
      241 ggcggacgtc ctgccatgga tgcagtgaaa aagggattgc cgcacgcgcc ggaattgatc
      301 agaagagtca atcgccgtat tggcgaacgc acgtcccatc gcgttgcctc tagatcccag
      361 ctagtgaaat ctgaattgga agagaagaaa tctgaactta gacataaatt gaaatatgtg
      421 ccacatgaat atattgaatt gattgaaatc gcaagaaatt caactcagga tagaatcctt
      481 gaaatgaagg tgatggagtt ctttatgaag gtttatggtt atcgtggtaa acatttgggt
      541 ggatcaagga aaccagacgg agcaatttat actgtcggat ctcctattga ttacggtgtg
      601 atcgttgata ctaaggcata ttcaggaggt tataatcttc caattggtca agcagatgaa
      661 atgcaaagat atgtcgaaga gaatcaaaca agaaacaagc atatcaaccc taatgaatgg
      721 tggaaagtct atccatcttc agtaacagaa tttaagttct tgtttgtgag tggtcatttc
      781 aaaggaaact acaaagctca gcttacaaga ttgaatcata tcactaattg taatggagct
      841 gttcttagtg
//
