LOCUS       sequence-56640-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  destination vector for the Golden Gate TALEN kit, directs expression
            of TALENs from a truncated CAGs promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Carlson DF, Tan W, Lillico SG, Stverakova D, Proudfoot C, Christian 
            M, Voytas DF, Long CR, Whitelaw CB, Fahrenkrug SC
  TITLE     Efficient TALEN-mediated gene knockout in livestock.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Oct 1.
  PUBMED    23027955
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2012 Oct 1."
COMMENT     Sequence Label: pC-GoldyTALEN
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(16..35)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(17..35)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(45..62)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(45..61)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(54..76)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(637..663)
                     /codon_start=1
                     /product="epitope tag from the baculovirus Autographa 
                     californica GP64 envelope fusion protein"
                     /label=AcV5 tag
                     /translation="SWKDASGWS"
     CDS             complement(664..684)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(787..806)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
ORIGIN
        1 ccggtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
       61 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
      121 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
      181 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cccgtctcca ggggggcacc
      241 cgtcagtgca ttgcgcgatg catgcactgc ctccattgcg gtcacgccgc cacgttttgc
      301 aatcttcaca agttggcctg tgtccaactg taacggcgga cctctcaact cccccgcatc
      361 cgtgagcaag gcctccaggg cgcgtgcgcc ggaccactgt ttgccgacgc caacgatgtc
      421 ttcgtgtgtc gcctctggca acgccgtgat tatgtgctga tacgtgacag cgacggtccc
      481 taacgctgcc gggtgttggc tgagcgcaac gatgtgcgcg tgtgtaaacc catggcccac
      541 cagtgcctcg tggtgctgcg ccactgtcga acgcaccttc ggtttgatct tctcttgctg
      601 ctgctgactg tagccgagcg tgcgtagatc cactctagac caaccacttg cgtccttcca
      661 actaaccttt ctctttttct ttggagggga ggaagccatt gttgaacctc ctattgttac
      721 gatatataca atatctttct cgagttagat cctagcttaa aattctttgc caaaatgatg
      781 agacagcaca ataaccagca cgttgcccag gagctgtagg aaaaagaaga
//
