LOCUS       sequence-56644-e        3199 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Dukkipati A, Park HH, Waghray D, Fischer S, Garcia KC
  TITLE     BacMam system for high-level expression of recombinant soluble and 
            membrane glycoproteins for structural studies.
  JOURNAL   Protein Expr Purif. 2008 Dec;62(2):160-70. Epub 2008 Aug 22.
  PUBMED    18782620
REFERENCE   2  (bases 1 to 3199)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2008-12"; volume: "62(2)"; pages: "160-70. Epub
            2008 Aug 22"
COMMENT     Sequence Label: pVLAD6L
FEATURES             Location/Qualifiers
     source          1..3199
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        289..668
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        669..872
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     823..843
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          1008..1140
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1123..1142
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1185..1203
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1219..1228
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1325..1348
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             1358..1393
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             1394..1417
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     misc_feature    1421..2009
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1474..1494)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1892..1903)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2021..2142
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2032..2051)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2086..2105
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(2276..2367)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(2334..2351)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
ORIGIN
        1 tgtcttccat gaagcgggtc caagtttccc tgtagaactc ttttcctttc catccaacga
       61 caagcttcat cgtgtcgggt tkaacattac ggatttcctt gaagagagtg agtttttggt
      121 tcttgccggg tcccaggaaa ggatcagatc ttcaatattg gccattagcc atattattca
      181 ttggttatat agcataaatc aatattggct attggccatt gcatacgttg tatctatatc
      241 ataatatgta catttatatt ggctcatgtc caatatgacc gccatgttgg cattgattat
      301 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
      361 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      421 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      481 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      541 tgccaagtcc gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      601 agtacatgac cttacgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      661 ttaccatggt gatgcggttt tggcagtaca ccaatgggcg tggatagcgg tttgactcac
      721 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      781 aacgggactt tccaaaatgt cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc
      841 gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atcactagaa
      901 gctttattgc ggtagtttat cacagttaaa ttgctaacgc agtcagtgct tctgacacaa
      961 cagtctcgaa cttaagctgc agaagttggt cgtgaggcac tgggcaggta agtatcaagg
     1021 ttacaagaca ggtttaagga gaccaataga aactgggctt gtcgagacag agaagactct
     1081 tgcgtttctg ataggcacct attggtctta ctgacatcca ctttgccttt ctctccacag
     1141 gtgtccactc ccagttcaat tacagctctt aaggctagag tacttaatac gactcactat
     1201 aggctagcct cgagaattgc caccatggat atgagagttc ctgctcaatt gctggggttg
     1261 ctcttgctct ggtttcctgg cgaattcacg cgtggtacct ctagagtcga cccgggcggc
     1321 cgcattggaa gttctgtttc aagggcctgg ggccgcagaa gatcaagttg atccaagatt
     1381 gattgatggc aagcatcacc atcaccatca ccatcactag aatcaacctc tggattacaa
     1441 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     1501 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     1561 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     1621 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     1681 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     1741 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     1801 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat
     1861 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     1921 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     1981 tcggatctcc ctttgggccg cctccccgcc tgagacatga taagatacat tgatgagttt
     2041 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     2101 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
     2161 cattttatgt ttcaggttca gggggagatg tgggaggttt tttaaagcaa gtaaaacctc
     2221 tacaaatgtg gtaggatccg cgcccgatgg tgggacggta tgaataatcc ggaatattta
     2281 taggtttttt tattacaaaa ctgttacgaa aacagtaaaa tacttattta tttgcgagat
     2341 ggttatcatt ttaattatct ccatgatatc agcaactata tattgataga catttccagt
     2401 ttgtgatatt agtttgtgcg tctcattaca atggctgtta tttttaacaa caaacaactg
     2461 ctcgcagaca atagtataga aaagggaggt gaactgtttt tgtttaacgg ttcgtacaac
     2521 attttggaaa gttatgttaa tccggtgctg ctaaaaaatg gtgtaattga actagaagaa
     2581 gctgcgtact atgccggcaa catattgtac aaaaccgacg atcccaaatt ccttgattat
     2641 ataaatttaa taattaaagc aacacactcc gaagaactac cagaaaatag cactgttgta
     2701 aattacagaa aaactatgcg cagcggtact atacacccca ttaaaaaaga catatatatt
     2761 tatgacanca aaaaatttac tctatacgat agatacatat atggatacga taataactat
     2821 gttaattttt atgaggagaa aaatgaaaaa aangakanar raawacranr gmwsattack
     2881 accaggcgtc tagtttatgt gaaaataana ttatattgtc gcaaattaac tgtgaattat
     2941 ttgaaatcat ttaatattac cttcacgata taacnagcgt nttattatag ataanactac
     3001 aatgttctgt tgcgtttggt tgtatcgtta taaaaacaaa tttgacattt atattgttta
     3061 tattcaaaat tacaatagat tgagaccttg ncgtgcgcca acgaacgagc tgtcaaagtt
     3121 tcttttttgg cgtagaggtt aacntctggg aatgataana agntgactcc ccccctggag
     3181 cngnngaaat nnnaanctg
//
