LOCUS       sequence-56645-e        3760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Dukkipati A, Park HH, Waghray D, Fischer S, Garcia KC
  TITLE     BacMam system for high-level expression of recombinant soluble and 
            membrane glycoproteins for structural studies.
  JOURNAL   Protein Expr Purif. 2008 Dec;62(2):160-70. Epub 2008 Aug 22.
  PUBMED    18782620
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2008-12"; volume: "62(2)"; pages: "160-70. Epub
            2008 Aug 22"
COMMENT     Sequence Label: pVLAD8
FEATURES             Location/Qualifiers
     source          1..3760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        289..668
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        669..872
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     823..843
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          1008..1140
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1123..1142
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1185..1203
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1257..1280
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             1968..1985
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    1989..2577
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2042..2062)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2460..2471)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2589..2710
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2600..2619)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2654..2673
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(2844..2935)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(2902..2919)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     misc_recomb     2939..3524
                     /label=baculovirus recombination region (ORF603)
                     /note="contains ORF603 and part of lef2"
ORIGIN
        1 tgtcttccat gaagcgggtc caagtttccc tgtagaactc ttttcctttc catccaacga
       61 caagcttcat cgtgtcgggt ttaacattac ggatttcctt gaagagagtg agtttttggt
      121 tcttgccggg tcccaggaaa ggatcagatc ttcaatattg gccattagcc atattattca
      181 ttggttatat agcataaatc aatattggct attggccatt gcatacgttg tatctatatc
      241 ataatatgta catttatatt ggctcatgtc caatatgacc gccatgttgg cattgattat
      301 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
      361 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      421 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      481 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      541 tgccaagtcc gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      601 agtacatgac cttacgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      661 ttaccatggt gatgcggttt tggcagtaca ccaatgggcg tggatagcgg tttgactcac
      721 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      781 aacgggactt tccaaaatgt cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc
      841 gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atcactagaa
      901 gctttattgc ggtagtttat cacagttaaa ttgctaacgc agtcagtgct tctgacacaa
      961 cagtctcgaa cttaagctgc agaagttggt cgtgaggcac tgggcaggta agtatcaagg
     1021 ttacaagaca ggtttaagga gaccaataga aactgggctt gtcgagacag agaagactct
     1081 tgcgtttctg ataggcacct attggtctta ctgacatcca ctttgccttt ctctccacag
     1141 gtgtccactc ccagttcaat tacagctctt aaggctagag tacttaatac gactcactat
     1201 aggctagcct cgagaattca cgcgtggtac ctctagagtc gacccgggcg gccgcattgg
     1261 aagttctgtt tcaagggcct gggagcgaca aaactcacac atgcccaccg tgcccagcac
     1321 ctgaactcct ggggggaccg tcagtcttcc tcttcccccc aaaacccaag gacaccctca
     1381 tgatctcccg gacccctgag gtcacatgcg tggtggtgga cgtgagccac gaagaccctg
     1441 aggtcaagtt caactggtac gtggacggcg tggaggtgca taatgccaag acaaagccgc
     1501 gggaggagca gtacaacagc acgtaccgtg tggtcagcgt cctcaccgtc ctgcaccagg
     1561 actggctgaa tggcaaggag tacaagtgca aggtctccaa caaagccctc ccagccccca
     1621 tcgagaaaac catctccaaa gccaaagggc agccccgaga accacaggtg tacaccctgc
     1681 ccccatcccg ggaggagatg accaagaacc aggtcagcct gacctgcctg gtcaaaggct
     1741 tctatcccag cgacatcgcc gtggagtggg agagcaatgg gcagccggag aacaactaca
     1801 agaccacgcc tcccgtgctg gactccgacg gctccttctt cctctacagc aagctcaccg
     1861 tggacaagag caggtggcag caggggaacg tcttctcatg ctccgtgatg catgagggtc
     1921 tgcacaacca ctacacgcag aagagcctct ccctgtctcc gggtaaacac catcaccatc
     1981 accattagaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     2041 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     2101 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     2161 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     2221 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     2281 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     2341 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
     2401 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     2461 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     2521 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     2581 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     2641 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     2701 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggagatgtg
     2761 ggaggttttt taaagcaagt aaaacctcta caaatgtggt aggatccgcg cccgatggtg
     2821 ggacggtatg aataatccgg aatatttata ggttttttta ttacaaaact gttacgaaaa
     2881 cagtaaaata cttatttatt tgcgagatgg ttatcatttt aattatctcc atgatatcag
     2941 caactatata ttgatagaca tttccagttt gtgatattag tttgtgcgtc tcattacaat
     3001 ggctgttatt tttaacaaca aacaactgct cgcagacaat agtatagaaa agggaggtga
     3061 actgtttttg tttaacggtt cgtacaacat tttggaaagt tatgttaatc cggtgctgct
     3121 aaaaaatggt gtaattgaac tagaagaagc tgcgtactat gccggcaaca tattgtacaa
     3181 aaccgacgat cccaaattca ttgattatat aaatttaata attaaagcaa cacactccga
     3241 agaactacca gaaaatagca ctgttgtaaa ttacagaaaa actatgcgca gcggtactat
     3301 acaccccatt aaaaagacat atatatttat gacacaaaaa atttactcta tacgatagat
     3361 acatatatgg atacgataat aactatgtta atttttatga ggagaaaaat gaaaaagaga
     3421 aggaatacga agaagaagac gacaaggcgt ctagtttatg tgaaaataaa attatattgt
     3481 cgcaaattaa ctgtgaatca tttgaaaatg attttaaata ttacctcagc gattataact
     3541 acgcgttttc aattatagat aatactacaa atgttcttgt tgcgtttggt ttgtatcgtt
     3601 aataaaaaaa caaatttgac atttataatt gttttattat canaaattac aataggattg
     3661 agacccttgc agtgccagca acggacagag cttgncgacn gtttnnattt ttggcagttn
     3721 nnnnttannn cnncatcngg gaattgaata aaaanatgca
//
