LOCUS       sequence-56646-e        3751 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Dukkipati A, Park HH, Waghray D, Fischer S, Garcia KC
  TITLE     BacMam system for high-level expression of recombinant soluble and 
            membrane glycoproteins for structural studies.
  JOURNAL   Protein Expr Purif. 2008 Dec;62(2):160-70. Epub 2008 Aug 22.
  PUBMED    18782620
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2008-12"; volume: "62(2)"; pages: "160-70. Epub
            2008 Aug 22"
COMMENT     Sequence Label: pVLAD8L
FEATURES             Location/Qualifiers
     source          1..3751
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        214..593
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        594..797
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     748..768
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     794..818
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          933..1065
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1048..1067
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1110..1128
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1144..1153
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1250..1273
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             1961..1978
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    1982..2570
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2035..2055)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2453..2464)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2582..2703
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2593..2612)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2647..2666
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(2837..2928)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(2895..2912)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     misc_recomb     2932..3520
                     /label=baculovirus recombination region (ORF603)
                     /note="contains ORF603 and part of lef2"
ORIGIN
        1 gtggccagta cttacggatt tccttgaaga gagtgagttt ttggttcttg ccgggtccca
       61 ggaaaggatc agatcttcaa tattggccat tagccatatt attcattggt tatatagcat
      121 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      181 atattggctc atgtccaata tgaccgccat gttggcattg attattgact agttattaat
      241 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      301 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      361 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      421 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      481 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      541 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      601 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      661 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      721 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      781 tctatataag cagagctcgt ttagtgaacc gtcagatcac tagaagcttt attgcggtag
      841 tttatcacag ttaaattgct aacgcagtca gtgcttctga cacaacagtc tcgaacttaa
      901 gctgcagaag ttggtcgtga ggcactgggc aggtaagtat caaggttaca agacaggttt
      961 aaggagacca atagaaactg ggcttgtcga gacagagaag actcttgcgt ttctgatagg
     1021 cacctattgg tcttactgac atccactttg cctttctctc cacaggtgtc cactcccagt
     1081 tcaattacag ctcttaaggc tagagtactt aatacgactc actataggct agcctcgaga
     1141 attgccacca tggatatgag agttcctgct caattgctgg ggttgctctt gctctggttt
     1201 cctggcgaat tcacgcgtgg tacctctaga gtcgacccgg gcggccgcat tggaagttct
     1261 gtttcaaggg cctgggagcg acaaaactca cacatgccca ccgtgcccag cacctgaact
     1321 cctgggggga ccgtcagtct tcctcttccc cccaaaaccc aaggacaccc tcatgatctc
     1381 ccggacccct gaggtcacat gcgtggtggt ggacgtgagc cacgaagacc ctgaggtcaa
     1441 gttcaactgg tacgtggacg gcgtggaggt gcataatgcc aagacaaagc cgcgggagga
     1501 gcagtacaac agcacgtacc gtgtggtcag cgtcctcacc gtcctgcacc aggactggct
     1561 gaatggcaag gagtacaagt gcaaggtctc caacaaagcc ctcccagccc ccatcgagaa
     1621 aaccatctcc aaagccaaag ggcagccccg agaaccacag gtgtacaccc tgcccccatc
     1681 ccgggaggag atgaccaaga accaggtcag cctgacctgc ctggtcaaag gcttctatcc
     1741 cagcgacatc gccgtggagt gggagagcaa tgggcagccg gagaacaact acaagaccac
     1801 gcctcccgtg ctggactccg acggctcctt cttcctctac agcaagctca ccgtggacaa
     1861 gagcaggtgg cagcagggga acgtcttctc atgctccgtg atgcatgagg gtctgcacaa
     1921 ccactacacg cagaagagcc tctccctgtc tccgggtaaa caccatcacc atcaccatta
     1981 gaatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     2041 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     2101 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     2161 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     2221 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     2281 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     2341 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
     2401 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     2461 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     2521 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctgagacatg
     2581 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     2641 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     2701 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggagat gtgggaggtt
     2761 ttttaaagca agtaaaacct ctacaaatgt ggtaggatcc gcgcccgatg gtgggacggt
     2821 atgaataatc cggaatattt ataggttttt ttattacaaa actgttacga aaacagtaaa
     2881 atacttattt atttgcgaga tggttatcat tttaattatc tccatgatat cagcaactat
     2941 atattgatag acatttccag tttgtgatat tagtttgtgc gtctcattac aatggctgtt
     3001 atttttaaca acaaacaact gctcgcagac aatagtatag aaaagggagg tgaactgttt
     3061 ttgtttaacg gttcgtacaa cattttggaa agttatgtta atccggtgct gctaaaaaat
     3121 ggtgtaattg aactagaaga agctgcgtac tatgccggca acatattgta caaaaccgac
     3181 gatcccaaat tcattgatta tataaattta ataattaaag caacacactc cgaagaacta
     3241 ccagaaaata gcactgttgt aaattacaga aaaactatgc gcagcggtac tatacacccc
     3301 attaaaaaag acatatatat ttatgacaac aaaaaattta ctctatacga tagatacata
     3361 tatgggatac gataataact atgttaattt ttatgaggag aaaaatgaaa aagagaakga
     3421 atacgaagaa gaagacgaca aggcgtctag tttatgtgaa aataaaatta tattgtcgca
     3481 aattaactgt gaatcatttg aaaatgattt taaatattac ctcagcgatt ataactacgc
     3541 gttttcaatt atagataata ctacaaatgt tcttgttgcg tttggtttgt atcgttaata
     3601 aaaaacaaat ttgacattta taattgtttt attattcaat aattacaaat aggattgaga
     3661 cccttgcagt tgccagcaaa cggacagagc ttgtcgacag ttttcatttt ttggcagttt
     3721 ggtggtttat acacatcttg gatttgataa a
//
