LOCUS       sequence-56652-e       10096 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Non-integrating (episomal) expression of EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10096)
  AUTHORS   Okita K, Yamakawa T, Matsumura Y, Sato Y, Amano N, Watanabe A, 
            Goshima N, Yamanaka S
  TITLE     An Efficient Non-viral Method to Generate Integration-Free Human iPS
            Cells from Cord Blood and Peripheral Blood Cells.
  JOURNAL   Stem Cells. 2012 Nov 29. doi: 10.1002/stem.1293.
  PUBMED    23193063
REFERENCE   2  (bases 1 to 10096)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells.
            2012 Nov 29. doi: 10.1002/stem.1293."
COMMENT     Sequence Label: pCE-GFP
FEATURES             Location/Qualifiers
     source          1..10096
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(72..89)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(243..831)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(323..342)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1002..1793,1794..1862))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1625..1644
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1863..1967)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        1998..2377
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2379..2655
                     /label=chicken beta-actin promoter
     intron          2656..3672
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3596..3618
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3680..3699
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      3727..3736
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3733..3735,3736..3738,3739..4452)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3733..3735,3736..3738,3739..4449)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3778..3799)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4039..4058)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4386..4407
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4536..4555)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4601..4656
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4655..4674)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5017..5033)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5017..5033)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5030..5052)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5041..5057
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5065..5071,5072..5089,5090..5095))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5110..5131
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             5610..7535
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     rep_origin      7837..9626
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 gatccgctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
       61 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
      121 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
      181 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
      241 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      301 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      361 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
      421 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
      481 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
      541 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
      601 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
      661 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
      721 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
      781 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
      841 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
      901 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
      961 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     1021 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     1081 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     1141 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     1201 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     1261 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     1321 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     1381 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     1441 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     1501 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     1561 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     1621 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     1681 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     1741 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     1801 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     1861 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     1921 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     1981 aaagtgccac ctgggtcgac attgattatt gactagttat taatagtaat caattacggg
     2041 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     2101 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     2161 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     2221 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     2281 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     2341 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
     2401 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
     2461 attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgcgccag gcggggcggg
     2521 gcggggcgag gggcggggcg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc
     2581 gcgctccgaa agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga
     2641 agcgcgcggc gggcgggagt cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc
     2701 gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg
     2761 gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct tgtttctttt
     2821 ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc
     2881 tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc
     2941 ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag
     3001 gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc
     3061 tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg
     3121 caaccccccc tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg
     3181 ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg
     3241 gtgccgggcg gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc
     3301 ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa
     3361 tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg
     3421 aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag
     3481 gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag
     3541 cctcggggct gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc
     3601 ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc
     3661 tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa
     3721 gaattcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     3781 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     3841 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     3901 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     3961 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     4021 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     4081 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     4141 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     4201 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     4261 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     4321 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     4381 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     4441 ctgtacaagt aagaattcac tcctcaggtg caggctgcct atcagaaggt ggtggctggt
     4501 gtggccaatg ccctggctca caaataccac tgagatcttt ttccctctgc caaaaattat
     4561 ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc
     4621 attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca
     4681 aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg
     4741 ctggctgcca tgaacaaagg ttggctataa agaggtcatc agtatatgaa acagccccct
     4801 gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt
     4861 tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc
     4921 agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct cttatggaga
     4981 tccctcgacc tgcagcccaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     5041 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     5101 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     5161 gtcgggaaac ctgtcgtgcc agcggatctc aattctcatg tttgacagct tatcatcgat
     5221 aagctgatcc tcacaggccg cacccagctt ttcttccgtt gccccagtag catctctgtc
     5281 tggtgacctt gaagaggaag aggaggggtc ccgagaatcc ccatccctac cgtccagcaa
     5341 aaagggggac gaggaatttg aggcctggct tgaggctcag gacgcaaatc ttgaggatgt
     5401 tcagcgggag ttttccgggc tgcgagtaat tggtgatgag gacgaggatg gttcggagga
     5461 tggggaattt tcagacctgg atctgtctga cagcgaccat gaaggggatg agggtggggg
     5521 ggctgttgga gggggcagga gtctgcactc cctgtattca ctgagcgtcg tctaataaag
     5581 atgtctattg atctctttta gtgtgaatca tgtctgacga ggggccaggt acaggacctg
     5641 gaaatggcct aggagagaag ggagacacat ctggaccaga aggctccggc ggcagtggac
     5701 ctcaaagaag agggggtgat aaccatggac gaggacgggg aagaggacga ggacgaggag
     5761 gcggaagacc aggagccccg ggcggctcag gatcagggcc aagacataga gatggtgtcc
     5821 ggagacccca aaaacgtcca agttgcattg gctgcaaagg gacccacggt ggaacaggag
     5881 caggagcagg agcgggaggg gcaggagcag gaggggcagg agcaggagga ggggcaggag
     5941 caggaggagg ggcaggaggg gcaggagggg caggaggggc aggagcagga ggaggggcag
     6001 gagcaggagg aggggcagga ggggcaggag gggcaggagc aggaggaggg gcaggagcag
     6061 gaggaggggc aggaggggca ggagcaggag gaggggcagg aggggcagga ggggcaggag
     6121 caggaggagg ggcaggagca ggaggagggg caggaggggc aggagcagga ggaggggcag
     6181 gaggggcagg aggggcagga gcaggaggag gggcaggagc aggaggggca ggaggggcag
     6241 gaggggcagg agcaggaggg gcaggagcag gaggaggggc aggaggggca ggaggggcag
     6301 gagcaggagg ggcaggagca ggaggggcag gagcaggagg ggcaggagca ggaggggcag
     6361 gaggggcagg agcaggaggg gcaggagggg caggagcagg aggggcagga ggggcaggag
     6421 caggaggagg ggcaggaggg gcaggagcag gaggaggggc aggaggggca ggagcaggag
     6481 gggcaggagg ggcaggagca ggaggggcag gaggggcagg agcaggaggg gcaggagggg
     6541 caggagcagg aggaggggca ggagcaggag gggcaggagc aggaggtgga ggccggggtc
     6601 gaggaggcag tggaggccgg ggtcgaggag gtagtggagg ccggggtcga ggaggtagtg
     6661 gaggccgccg gggtagagga cgtgaaagag ccaggggggg aagtcgtgaa agagccaggg
     6721 ggagaggtcg tggacgtgga gaaaagaggc ccaggagtcc cagtagtcag tcatcatcat
     6781 ccgggtctcc accgcgcagg ccccctccag gtagaaggcc atttttccac cctgtagggg
     6841 aagccgatta ttttgaatac caccaagaag gtggcccaga tggtgagcct gacgtgcccc
     6901 cgggagcgat agagcagggc cccgcagatg acccaggaga aggcccaagc actggacccc
     6961 ggggtcaggg tgatggaggc aggcgcaaaa aaggagggtg gtttggaaag catcgtggtc
     7021 aaggaggttc caacccgaaa tttgagaaca ttgcagaagg tttaagagct ctcctggcta
     7081 ggagtcacgt agaaaggact accgacgaag gaacttgggt cgccggtgtg ttcgtatatg
     7141 gaggtagtaa gacctccctt tacaacctaa ggcgaggaac tgcccttgct attccacaat
     7201 gtcgtcttac accattgagt cgtctcccct ttggaatggc ccctggaccc ggcccacaac
     7261 ctggcccgct aagggagtcc attgtctgtt atttcatggt ctttttacaa actcatatat
     7321 ttgctgaggt tttgaaggat gcgattaagg accttgttat gacaaagccc gctcctacct
     7381 gcaatatcag ggtgactgtg tgcagctttg acgatggagt agatttgcct ccctggtttc
     7441 cacctatggt ggaaggggct gccgcggagg gtgatgacgg agatgacgga gatgaaggag
     7501 gtgatggaga tgagggtgag gaagggcagg agtgatgtaa cttgttagga gacgccctca
     7561 atcgtattaa aagccgtgta ttcccccgca ctaaagaata aatccccagt agacatcatg
     7621 cgtgctgttg gtgtatttct ggccatctgt cttgtcacca ttttcgtcct cccaacatgg
     7681 ggcaattggg catacccatg ttgtcacgtc actcagctcc gcgctcaaca ccttctcgcg
     7741 ttggaaaaca ttagcgacat ttacctggtg agcaatcaga catgcgacgg ctttagcctg
     7801 gcctccttaa attcacctaa gaatgggagc aaccagcagg aaaaggacaa gcagcgaaaa
     7861 ttcacgcccc cttgggaggt ggcggcatat gcaaaggata gcactcccac tctactactg
     7921 ggtatcatat gctgactgta tatgcatgag gatagcatat gctacccgga tacagattag
     7981 gatagcatat actacccaga tatagattag gatagcatat gctacccaga tatagattag
     8041 gatagcctat gctacccaga tataaattag gatagcatat actacccaga tatagattag
     8101 gatagcatat gctacccaga tatagattag gatagcctat gctacccaga tatagattag
     8161 gatagcatat gctacccaga tatagattag gatagcatat gctatccaga tatttgggta
     8221 gtatatgcta cccagatata aattaggata gcatatacta ccctaatctc tattaggata
     8281 gcatatgcta cccggataca gattaggata gcatatacta cccagatata gattaggata
     8341 gcatatgcta cccagatata gattaggata gcctatgcta cccagatata aattaggata
     8401 gcatatacta cccagatata gattaggata gcatatgcta cccagatata gattaggata
     8461 gcctatgcta cccagatata gattaggata gcatatgcta tccagatatt tgggtagtat
     8521 atgctaccca tggcaacatt agcccaccgt gctctcagcg acctcgtgaa tatgaggacc
     8581 aacaaccctg tgcttggcgc tcaggcgcaa gtgtgtgtaa tttgtcctcc agatcgcagc
     8641 aatcgcgccc ctatcttggc ccgcccacct acttatgcag gtattccccg gggtgccatt
     8701 agtggttttg tgggcaagtg gtttgaccgc agtggttagc ggggttacaa tcagccaagt
     8761 tattacaccc ttattttaca gtccaaaacc gcagggcggc gtgtgggggc tgacgcgtgc
     8821 ccccactcca caatttcaaa aaaaagagtg gccacttgtc tttgtttatg ggccccattg
     8881 gcgtggagcc ccgtttaatt ttcgggggtg ttagagacaa ccagtggagt ccgctgctgt
     8941 cggcgtccac tctctttccc cttgttacaa atagagtgta acaacatggt tcacctgtct
     9001 tggtccctgc ctgggacaca tcttaataac cccagtatca tattgcacta ggattatgtg
     9061 ttgcccatag ccataaattc gtgtgagatg gacatccagt ctttacggct tgtccccacc
     9121 ccatggattt ctattgttaa agatattcag aatgtttcat tcctacacta gtatttattg
     9181 cccaaggggt ttgtgagggt tatattggtg tcatagcaca atgccaccac tgaacccccc
     9241 gtccaaattt tattctgggg gcgtcacctg aaaccttgtt ttcgagcacc tcacatacac
     9301 cttactgttc acaactcagc agttattcta ttagctaaac gaaggagaat gaagaagcag
     9361 gcgaagattc aggagagttc actgcccgct ccttgatctt cagccactgc ccttgtgact
     9421 aaaatggttc actaccctcg tggaatcctg accccatgta aataaaaccg tgacagctca
     9481 tggggtggga gatatcgctg ttccttagga cccttttact aaccctaatt cgatagcata
     9541 tgcttcccgt tgggtaacat atgctattga attagggtta gtctggatag tatatactac
     9601 tacccgggaa gcatatgcta cccgtttagg gttaacaagg gggccttata aacactattg
     9661 ctaatgccct cttgagggtc cgcttatcgg tagctacaca ggcccctctg attgacgttg
     9721 gtgtagcctc ccgtagtctt cctgggcccc tgggaggtac atgtccccca gcattggtgt
     9781 aagagcttca gccaagagtt acacataaag gcaatgttgt gttgcagtcc acagactgca
     9841 aagtctgctc caggatgaaa gccactcagt gttggcaaat gtgcacatcc atttataagg
     9901 atgtcaacta cagtcagaga acccctttgt gtttggtccc cccccgtgtc acatgtggaa
     9961 cagggcccag ttggcaagtt gtaccaacca actgaaggga ttacatgcac tgccccgcga
    10021 agaaggggca gagatgtcgt agtcaggttt agttcgtccg gggcggggca tcgatcctct
    10081 agagtcgacg ctagcg
//
