LOCUS       sequence-56653-e       10514 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Non-integrating (episomal) expression of mouse p53DD - p53 
            carboxy-terminal dominant-negative fragment .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10514)
  AUTHORS   Okita K, Yamakawa T, Matsumura Y, Sato Y, Amano N, Watanabe A, 
            Goshima N, Yamanaka S
  TITLE     An Efficient Non-viral Method to Generate Integration-Free Human iPS
            Cells from Cord Blood and Peripheral Blood Cells.
  JOURNAL   Stem Cells. 2012 Nov 29. doi: 10.1002/stem.1293.
  PUBMED    23193063
REFERENCE   2  (bases 1 to 10514)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells.
            2012 Nov 29. doi: 10.1002/stem.1293."
COMMENT     Sequence Label: pCXLE-mp53DD
FEATURES             Location/Qualifiers
     source          1..10514
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        3..382
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        384..661
                     /label=chicken beta-actin promoter
     intron          662..1678
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1602..1624
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1686..1705
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     misc_feature    2091..2679
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2144..2164)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2562..2573)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2770..2789)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2835..2890
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2889..2908)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3251..3267)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3251..3267)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3264..3286)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3275..3291
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3299..3305,3306..3323,3324..3329))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3344..3365
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    3454..3487
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             3948..5873
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     protein_bind    6041..6074
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      6261..8050
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(8591..8608)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8762..9350)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8842..8861)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9521..10312,10313..10381))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10144..10163
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10382..10486)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
       61 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
      121 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
      181 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
      241 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
      301 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
      361 attagtcatc gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat
      421 ctcccccccc tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc
      481 gatgggggcg gggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     1261 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
     1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1561 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1621 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcgcccttca
     1741 ccaggatgac tgccatggag gagtcacagt cggatatcag cctcaagaga gcgctgccca
     1801 cctgcacaag cgcctctccc ccgcaaaaga aaaaaccact tgatggagag tatttcaccc
     1861 tcaagatccg cgggcgtaaa cgcttcgaga tgttccggga gctgaatgag gccttagagt
     1921 taaaggatgc ccatgctaca gaggagtctg gagacagcag ggctcactcc agctacctga
     1981 agaccaagaa gggccagtct acttcccgcc ataaaaaaac aatggtcaag aaagtggggc
     2041 ctgactcaga ctgaagggcg aattcaagct tcggggacta gtcatatgat aatcaacctc
     2101 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     2161 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     2221 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     2281 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     2341 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     2401 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     2461 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg
     2521 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     2581 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     2641 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcggtacaat tcactcctca
     2701 ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc aatgccctgg ctcacaaata
     2761 ccactgagat ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca
     2821 tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt
     2881 gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagta
     2941 tttggtttag agtttggcaa catatgccca tatgctggct gccatgaaca aaggttggct
     3001 ataaagaggt catcagtata tgaaacagcc ccctgctgtc cattccttat tccatagaaa
     3061 agccttgact tgaggttaga ttttttttat attttgtttt gtgttatttt tttctttaac
     3121 atccctaaaa ttttccttac atgttttact agccagattt ttcctcctct cctgactact
     3181 cccagtcata gctgtccctc ttctcttatg gagatccctc gacctgcagc ccaagcttgg
     3241 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     3301 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     3361 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga
     3421 tctcaattcc gatcatattc aataaccctt aatataactt cgtataatgt atgctatacg
     3481 aagttattag gtctgaagag gagtttacgt ccagccaagc ttaggatcaa ttctcatgtt
     3541 tgacagctta tcatcgataa gctgatcctc acaggccgca cccagctttt cttccgttgc
     3601 cccagtagca tctctgtctg gtgaccttga agaggaagag gaggggtccc gagaatcccc
     3661 atccctaccg tccagcaaaa agggggacga ggaatttgag gcctggcttg aggctcagga
     3721 cgcaaatctt gaggatgttc agcgggagtt ttccgggctg cgagtaattg gtgatgagga
     3781 cgaggatggt tcggaggatg gggaattttc agacctggat ctgtctgaca gcgaccatga
     3841 aggggatgag ggtggggggg ctgttggagg gggcaggagt ctgcactccc tgtattcact
     3901 gagcgtcgtc taataaagat gtctattgat ctcttttagt gtgaatcatg tctgacgagg
     3961 ggccaggtac aggacctgga aatggcctag gagagaaggg agacacatct ggaccagaag
     4021 gctccggcgg cagtggacct caaagaagag ggggtgataa ccatggacga ggacggggaa
     4081 gaggacgagg acgaggaggc ggaagaccag gagccccggg cggctcagga tcagggccaa
     4141 gacatagaga tggtgtccgg agaccccaaa aacgtccaag ttgcattggc tgcaaaggga
     4201 cccacggtgg aacaggagca ggagcaggag cgggaggggc aggagcagga ggggcaggag
     4261 caggaggagg ggcaggagca ggaggagggg caggaggggc aggaggggca ggaggggcag
     4321 gagcaggagg aggggcagga gcaggaggag gggcaggagg ggcaggaggg gcaggagcag
     4381 gaggaggggc aggagcagga ggaggggcag gaggggcagg agcaggagga ggggcaggag
     4441 gggcaggagg ggcaggagca ggaggagggg caggagcagg aggaggggca ggaggggcag
     4501 gagcaggagg aggggcagga ggggcaggag gggcaggagc aggaggaggg gcaggagcag
     4561 gaggggcagg aggggcagga ggggcaggag caggaggggc aggagcagga ggaggggcag
     4621 gaggggcagg aggggcagga gcaggagggg caggagcagg aggggcagga gcaggagggg
     4681 caggagcagg aggggcagga ggggcaggag caggaggggc aggaggggca ggagcaggag
     4741 gggcaggagg ggcaggagca ggaggagggg caggaggggc aggagcagga ggaggggcag
     4801 gaggggcagg agcaggaggg gcaggagggg caggagcagg aggggcagga ggggcaggag
     4861 caggaggggc aggaggggca ggagcaggag gaggggcagg agcaggaggg gcaggagcag
     4921 gaggtggagg ccggggtcga ggaggcagtg gaggccgggg tcgaggaggt agtggaggcc
     4981 ggggtcgagg aggtagtgga ggccgccggg gtagaggacg tgaaagagcc agggggggaa
     5041 gtcgtgaaag agccaggggg agaggtcgtg gacgtggaga aaagaggccc aggagtccca
     5101 gtagtcagtc atcatcatcc gggtctccac cgcgcaggcc ccctccaggt agaaggccat
     5161 ttttccaccc tgtaggggaa gccgattatt ttgaatacca ccaagaaggt ggcccagatg
     5221 gtgagcctga cgtgcccccg ggagcgatag agcagggccc cgcagatgac ccaggagaag
     5281 gcccaagcac tggaccccgg ggtcagggtg atggaggcag gcgcaaaaaa ggagggtggt
     5341 ttggaaagca tcgtggtcaa ggaggttcca acccgaaatt tgagaacatt gcagaaggtt
     5401 taagagctct cctggctagg agtcacgtag aaaggactac cgacgaagga acttgggtcg
     5461 ccggtgtgtt cgtatatgga ggtagtaaga cctcccttta caacctaagg cgaggaactg
     5521 cccttgctat tccacaatgt cgtcttacac cattgagtcg tctccccttt ggaatggccc
     5581 ctggacccgg cccacaacct ggcccgctaa gggagtccat tgtctgttat ttcatggtct
     5641 ttttacaaac tcatatattt gctgaggttt tgaaggatgc gattaaggac cttgttatga
     5701 caaagcccgc tcctacctgc aatatcaggg tgactgtgtg cagctttgac gatggagtag
     5761 atttgcctcc ctggtttcca cctatggtgg aaggggctgc cgcggagggt gatgacggag
     5821 atgacggaga tgaaggaggt gatggagatg agggtgagga agggcaggag tgatgtaact
     5881 tgttaggaga cgccctcaat cgtattaaaa gccgtgtatt cccccgcact aaagaataaa
     5941 tccccagtag acatcatgcg tgctgttggt gtatttctgg ccatctgtct tgtcaccatt
     6001 ttcgtcctcc caacatgggg caattgccgg aacccttaat ataacttcgt ataatgtatg
     6061 ctatacgaag ttattaggtc cctcgaagag gttcactagc ggatctcaat tgggcatacc
     6121 catgttgtca cgtcactcag ctccgcgctc aacaccttct cgcgttggaa aacattagcg
     6181 acatttacct ggtgagcaat cagacatgcg acggctttag cctggcctcc ttaaattcac
     6241 ctaagaatgg gagcaaccag caggaaaagg acaagcagcg aaaattcacg cccccttggg
     6301 aggtggcggc atatgcaaag gatagcactc ccactctact actgggtatc atatgctgac
     6361 tgtatatgca tgaggatagc atatgctacc cggatacaga ttaggatagc atatactacc
     6421 cagatataga ttaggatagc atatgctacc cagatataga ttaggatagc ctatgctacc
     6481 cagatataaa ttaggatagc atatactacc cagatataga ttaggatagc atatgctacc
     6541 cagatataga ttaggatagc ctatgctacc cagatataga ttaggatagc atatgctacc
     6601 cagatataga ttaggatagc atatgctatc cagatatttg ggtagtatat gctacccaga
     6661 tataaattag gatagcatat actaccctaa tctctattag gatagcatat gctacccgga
     6721 tacagattag gatagcatat actacccaga tatagattag gatagcatat gctacccaga
     6781 tatagattag gatagcctat gctacccaga tataaattag gatagcatat actacccaga
     6841 tatagattag gatagcatat gctacccaga tatagattag gatagcctat gctacccaga
     6901 tatagattag gatagcatat gctatccaga tatttgggta gtatatgcta cccatggcaa
     6961 cattagccca ccgtgctctc agcgacctcg tgaatatgag gaccaacaac cctgtgcttg
     7021 gcgctcaggc gcaagtgtgt gtaatttgtc ctccagatcg cagcaatcgc gcccctatct
     7081 tggcccgccc acctacttat gcaggtattc cccggggtgc cattagtggt tttgtgggca
     7141 agtggtttga ccgcagtggt tagcggggtt acaatcagcc aagttattac acccttattt
     7201 tacagtccaa aaccgcaggg cggcgtgtgg gggctgacgc gtgcccccac tccacaattt
     7261 caaaaaaaag agtggccact tgtctttgtt tatgggcccc attggcgtgg agccccgttt
     7321 aattttcggg ggtgttagag acaaccagtg gagtccgctg ctgtcggcgt ccactctctt
     7381 tccccttgtt acaaatagag tgtaacaaca tggttcacct gtcttggtcc ctgcctggga
     7441 cacatcttaa taaccccagt atcatattgc actaggatta tgtgttgccc atagccataa
     7501 attcgtgtga gatggacatc cagtctttac ggcttgtccc caccccatgg atttctattg
     7561 ttaaagatat tcagaatgtt tcattcctac actagtattt attgcccaag gggtttgtga
     7621 gggttatatt ggtgtcatag cacaatgcca ccactgaacc ccccgtccaa attttattct
     7681 gggggcgtca cctgaaacct tgttttcgag cacctcacat acaccttact gttcacaact
     7741 cagcagttat tctattagct aaacgaagga gaatgaagaa gcaggcgaag attcaggaga
     7801 gttcactgcc cgctccttga tcttcagcca ctgcccttgt gactaaaatg gttcactacc
     7861 ctcgtggaat cctgacccca tgtaaataaa accgtgacag ctcatggggt gggagatatc
     7921 gctgttcctt aggacccttt tactaaccct aattcgatag catatgcttc ccgttgggta
     7981 acatatgcta ttgaattagg gttagtctgg atagtatata ctactacccg ggaagcatat
     8041 gctacccgtt tagggttaac aagggggcct tataaacact attgctaatg ccctcttgag
     8101 ggtccgctta tcggtagcta cacaggcccc tctgattgac gttggtgtag cctcccgtag
     8161 tcttcctggg cccctgggag gtacatgtcc cccagcattg gtgtaagagc ttcagccaag
     8221 agttacacat aaaggcaatg ttgtgttgca gtccacagac tgcaaagtct gctccaggat
     8281 gaaagccact cagtgttggc aaatgtgcac atccatttat aaggatgtca actacagtca
     8341 gagaacccct ttgtgtttgg tccccccccg tgtcacatgt ggaacagggc ccagttggca
     8401 agttgtacca accaactgaa gggattacat gcactgcccc gcgaagaagg ggcagagatg
     8461 tcgtagtcag gtttagttcg tccggggcgg ggatcgatcc tctagagtcg acgctagcgg
     8521 atccgctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     8581 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     8641 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     8701 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     8761 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     8821 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     8881 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     8941 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     9001 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     9061 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     9121 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     9181 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     9241 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     9301 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     9361 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     9421 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     9481 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     9541 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     9601 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     9661 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     9721 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     9781 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     9841 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     9901 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     9961 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
    10021 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
    10081 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
    10141 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    10201 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    10261 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    10321 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    10381 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    10441 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    10501 aagtgccacc tggg
//
