LOCUS       sequence-56666-e         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Fitzgerald KA, Rowe DC, Barnes BJ, Caffrey DR, Visintin A, Latz E, 
            Monks B, Pitha PM, Golenbock DT
  TITLE     LPS-TLR4 signaling to IRF-3/7 and NF-kappaB involves the toll 
            adapters TRAM and TRIF.
  JOURNAL   J Exp Med. 2003 Oct 6;198(7):1043-55. Epub 2003 Sep 29.
  PUBMED    14517278
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2003-10-6"; volume: "198(7)"; pages: "1043-55. Epub 
            2003 Sep 29"
COMMENT     Sequence Label: pEF-Bos TRAM Flag
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(82..99)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(100..123)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(133..144)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 atttcttgtc tcccacgtgg gagacctgat actctcaagg gtcccaggtg gggaccctca
       61 ctctagagtc gcggccgctc agtggtggtg gtggtggtgc ttgtcatcgt cgtccttgta
      121 gtccttgacg atccttcctt caatggatcc ggcaataaat tgtctttgta ccatatttct
      181 tgtctctttc catatagttt gttgtgtctt atacacagac tcctgaaaaa ttctttctac
      241 ttgtgtagga aatccacgac tttcttcctc taaggcattg atggtttgga gggcaaaggg
      301 agtcctttct cggggaaggg gattgttcag gggccgcatg ggtataacag agttgtattt
      361 atgctgcctg ttaacggagt tcattaggga cgtatagaac tggaaattac accaagtatc
      421 tcttaaaaag ttttcagtca gtaataagat tgtccatgca gacccattta cagcatcatc
      481 taaattctgt aaatgctgtc tgccacatgg catctcagca aagattattc cgggtttgat
      541 accaaagtca tcttgtagca gattctggac tctgagggct tcatctgtgt catcttctgc
      601 atgcaatatc acaaatttga ggaacacctc ttcttcagct tcttcttcaa acatctcttc
      661 cacgctctga gctccctcct gctttcctgt tggcccctct gttgtattgc tgtgctcagc
      721 aacattacac aaggatagat cttcagactt cttggaatct gactcatgat atcctggact
      781 tgtatccaca ctgtgccttt taccccaaga gagagaaaga gggcaggaat ttattttaga
      841 cttcccgata cccatctcga ggtcgaggga tctctagaga attcctcacg acacctgaaa
      901 tggaagaaaa a
//
