LOCUS       sequence-56667-e         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Fitzgerald KA, McWhirter SM, Faia KL, Rowe DC, Latz E, Golenbock DT,
            Coyle AJ, Liao SM, Maniatis T
  TITLE     IKKepsilon and TBK1 are essential components of the IRF3 signaling 
            pathway.
  JOURNAL   Nat Immunol. 2003 May;4(5):491-6.
  PUBMED    12692549
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Immunol."; date: "2003-05"; volume: "4(5)"; pages: "491-6"
COMMENT     Sequence Label: pEF-Bos MAL Flag
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(82..99)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(100..123)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(133..144)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(919..939)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 atttcttgtc tcccacgtgg gagacctgat actctcaagg gtcccaggtg gggaccctca
       61 ctctagagtc gcggccgctc agtggtggtg gtggtggtgc ttgtcatcgt cgtccttgta
      121 gtccttgacg atccttcctt caatggatcc aagtagatca gatactgtag ctgaatcccg
      181 ttccccttct ccctcctgta gtagcttaca acgcatgaca gcttctttga cttgacgaaa
      241 gccaccatca gggcccctgc catcgacgta gtacatgaat cggagctcag gtgggtaggc
      301 agctctgctg aggcccgaca gcagggggat ggtgcagccc tcggcccctg gagcctcggt
      361 cagggcctgc agcatctggt acttgcacca ggggtcctga aggaagcccg gcgtgatgag
      421 cagcacccgg cagtgactac tgctcagtgc ctggcacagc tcggacacta tagcgccgcc
      481 tggggttgca tcccggagtt gcaggaagca gcgcaggctg gcagtgctgc cttccaagta
      541 ggagaccagg tcctgggcgg ccaccaggtc ttcctcactg tggcacacgc agacgtcata
      601 gtctttgctc cagcgactac tgccactgtc actcgcatgt gtgggtggca ggctgggaga
      661 cgtgactgag ctgaggcttg ggggtagtgg gttgtcctgt gaggtaggct gtgaagcatc
      721 gctggaggtg ctttctgggg agttgggcct cttcttgggc ttcttcagca gggtctgcct
      781 gaaccagtca gccatcttgc ctagaggctt cttaggccga gagccaggag ctgggaggga
      841 ggtcgatgat gccatactcg aggtcgaggg atctctagag aattcctcac gacacctgaa
      901 atggaagaaa aaaactttga accactgtct gaggcttgag a
//
