LOCUS       sequence-56670-e         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Fitzgerald KA, McWhirter SM, Faia KL, Rowe DC, Latz E, Golenbock DT,
            Coyle AJ, Liao SM, Maniatis T
  TITLE     IKKepsilon and TBK1 are essential components of the IRF3 signaling 
            pathway.
  JOURNAL   Nat Immunol. 2003 May;4(5):491-6.
  PUBMED    12692549
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Immunol."; date: "2003-05"; volume: "4(5)"; pages: "491-6"
COMMENT     Sequence Label: pEF-Bos TRIF Flag
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(84..101)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(102..125)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(135..146)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ggatttcttg tctcccacgt gggagacctg atactctcaa gggtcccagg tggggaccct
       61 cactctagag tcgcggccgc tcagtggtgg tggtggtggt gcttgtcatc gtcgtccttg
      121 tagtccttga cgatccttcc ttcaatggat ctttctgcct cctgcgtctt gtcctcgggc
      181 gcctgggacc ctctctggtt ccacatgtgg ttgttcagcc ccagctgtac catctgtgcg
      241 tggtggataa tgaggggttg cagccctggg ctctgagggg gtgcgggtga ggccgtaggg
      301 aaggctgggg actgcgggaa gggcagtgac tgtggaaagg ctgctggctg tggggagggc
      361 ggtggggggg tgccagcctg ccatgcgtgc aggggtggcg gctgcgggca ccccggccaa
      421 gtgggaaagg gtggcggggc tcccaggggc acctggcccc caaagggcat tcgagcccca
      481 tagggcgccc cagtcccaaa tgacatgtgg ctcccaaaag ccacctggag ctgctccatc
      541 tgtgcctggt aggacaagta gctctggagg taggctgagt aggctgcgtt cagtgccgcc
      601 gcctgcatcc gctcaccgtc caggtgttgg ctctgttccc gcagggctcg ggtgtcctgt
      661 tccttcctcc acatggcctt tcgggcctga agcctgtggg gcttgaaggt gttggccacc
      721 ttcctggcga agatctggga gtgttcgtcc agccgcacca gcccggagag caggctggcc
      781 gtgtcgga
//
