LOCUS       sequence-56678-e         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Zilio N, Wehrkamp-Richter S, Boddy MN
  TITLE     A new versatile system for rapid control of gene expression in the 
            fission yeast Schizosaccharomyces pombe.
  JOURNAL   Yeast. 2012 Aug 29. doi: 10.1002/yea.2920.
  PUBMED    22968950
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2012
            Aug 29. doi: 10.1002/yea.2920."
COMMENT     Sequence Label: pFA6a-kanMX-tetO-Pcyc1-3xHA
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(36..223)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     144..168
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(256..345)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     protein_bind    571..589
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    613..631
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    655..673
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    697..715
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    739..757
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    781..799
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 ggatctatat taccctgtta tccctagcgg atctgccggt agaggtgtgg tcaataagag
       61 cgacctcata ctatacctga gaaagcaacc tgacctacag gaaagagtta ctcaagaata
      121 agaattttcg ttttaaaacc taagagtcac tttaaaattt gtatacactt atttttttta
      181 taacttattt aataataaaa atcataaatc ataagaaatt cgcttattta gaagtggcgc
      241 gcctcagcac tgagcagcgt aatctggaac gtcatatgga taggatcctg catagtccgg
      301 gacgtcatag ggatagcccg catagtcagg aacatcgtat gggtaaaaga tgttaattaa
      361 catcctccag gatgataaac ggatcccccg aattgatccg gtaatttagt gtgtgtattt
      421 gtgtttgcgt gtctatagaa gtatagtaat ttatgctaca aaggacctaa tgtataagga
      481 aagaatattt agagaaaaga agaaaacaag agttttatat acatacagag cacatgcatg
      541 ccatagggta ccgagctcga ctttcacttt tctctatcac tgatagggag tggtaaactc
      601 gactttcact tttctctatc actgataggg agtggtaaac tcgactttca cttttctcta
      661 tcactgatag ggagtggtaa actcgacttt cacttttctc tatcactgat agggagtggt
      721 aaactcgact ttcacttttc tctatcactg atagggagtg gtaaactcga ctttcacttt
      781 tctctatcac tgatagggag tggtaaactc gactttcact tttctc
//
