LOCUS       sequence-56680-e         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Zilio N, Wehrkamp-Richter S, Boddy MN
  TITLE     A new versatile system for rapid control of gene expression in the 
            fission yeast Schizosaccharomyces pombe.
  JOURNAL   Yeast. 2012 Aug 29. doi: 10.1002/yea.2920.
  PUBMED    22968950
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2012
            Aug 29. doi: 10.1002/yea.2920."
COMMENT     Sequence Label: pFA6a-kanMX-tetO-Pcyc1-GFP
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    join(255..273,274..296,297..315,316..338,339..357,358..380,
                     381..399,400..422,423..441,442..464,465..483,484..506,
                     507..525)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
ORIGIN
        1 gggacaactc cagtgaaaag ttcttctcct ttactgttaa ttaacatcct ccaggatgat
       61 aaacggatcc cccgaattga tccggtaatt tagtgtgtgt atttgtgttt gcgtgtctat
      121 agaagtatag taatttatgc tacaaaggac ctaatgtata aggaaagaat atttagagaa
      181 aagaagaaaa caagagtttt atatacatac agagcacatg catgccatag ggtaccgagc
      241 tcgactttca cttttctcta tcactgatag ggagtggtaa actcgacttt cacttttctc
      301 tatcactgat agggagtggt aaactcgact ttcacttttc tctatcactg atagggagtg
      361 gtaaactcga ctttcacttt tctctatcac tgatagggag tggtaaactc gactttcact
      421 tttctctatc actgataggg agtggtaaac tcgactttca cttttctcta tcactgatag
      481 ggagtggtaa actcgacttt cacttttctc tatcactgat agggagtggt aaactcgatc
      541 gaggaattga tctatattac ccagatctgt ttagcttgcc tcgtccccgc cgggtcaccc
      601 ggccagcgac atggaggccc agaataccct ccttgacagt cttgacgtgc gcagctcagg
      661 ggcatgatgt gactgtcgcc cgtacattta gcccatacat ccccatgtat aatcatttgc
      721 atccatacat tttgatggcc gcacggcgcg aagcaaaaat tacggctcct cgctgcagac
      781 ctgcgagcag ggaaacgctc ccctcacaga c
//
