LOCUS       sequence-56682-e         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Zilio N, Wehrkamp-Richter S, Boddy MN
  TITLE     A new versatile system for rapid control of gene expression in the 
            fission yeast Schizosaccharomyces pombe.
  JOURNAL   Yeast. 2012 Aug 29. doi: 10.1002/yea.2920.
  PUBMED    22968950
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2012
            Aug 29. doi: 10.1002/yea.2920."
COMMENT     Sequence Label: pFA6a-kanMX-tetO-Pcyc1-GFP
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(42..229)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     150..174
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(293..313)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 gtcgacggat ctatattacc ctgttatccc tagcggatct gccggtagag gtgtggtcaa
       61 taagagcgac ctcatactat acctgagaaa gcaacctgac ctacaggaaa gagttactca
      121 agaataagaa ttttcgtttt aaaacctaag agtcacttta aaatttgtat acacttattt
      181 tttttataac ttatttaata ataaaaatca taaatcataa gaaattcgct tatttagaag
      241 tggcgcgccc tatttgtata gttcatccat gccatgtgta atcccagcag ctgttacaaa
      301 ctcaagaagg accatgtggt ctctcttttc gttgggatct ttcgaaaggg cagattgtgt
      361 ggacaggtaa tggttgtctg gtaaaaggac agggccatcg ccaattggag tattttgttg
      421 ataatggtct gctagttgaa cgcttccatc ttcaatgttg tgtctaattt tgaagttaac
      481 tttgattcca ttcttttgtt tgtctgccat gatgtataca ttgtgtgagt tatagttgta
      541 ttccaatttg tgtccaagaa tgtttccatc ttctttaaaa tcaatacctt ttaactcgat
      601 tctattaaca agggtatcac cttcaaactt gacttcagca cgtgtcttgt agttcccgtc
      661 atctttgaaa aatatagttc tttcctgtac ataaccttcg ggcatggcac tcttgaaaaa
      721 gtcatgccgt ttcatatgat ctgggtatct tgaaaagcat tgaacaccat aagtgaaagt
      781 agtgacaagt gttggccatg gaacaggtag ttttccagta gtgcaaataa at
//
