LOCUS       sequence-56684-e         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive lentiviral expression, SV40-puro; PGK-gateway-V5 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  TITLE     Root Lab pLEX and pLIX Gateway backbones
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLEX_306
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(20..230)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      26..161
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             complement(461..505)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ctctagaagc tttttgcaaa gcctaggcct ccaaaaaagc ctcctcacta cttctggaat
       61 agctcagagg ccgaggcggc ctcggcctct gcataaataa aaaaaattag tcagccatgg
      121 ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt taggggcggg
      181 actatggttg ctgactaatt gagacgcatg ctttgcatac ttctgcctgc tggggagcct
      241 ggggactttc cacacctggt tgctgactaa ttgagatgca atgcatgggt cccctcgggg
      301 ttgggaggtg ggtctgaaac gataatggtg aatatccctg cctaactcta ttcactatag
      361 aaagtacagc aaaaactatt cttaaaccta ccaagcctcc tactatcatt atgaataatt
      421 ttatatacca cagccaattt gttatgttaa accaattcca caaacttgcc catttatcta
      481 attccaataa ttcttgttca ttcttttctt gctggttttg cgattcttca attaaggagt
      541 gtattaagct tgtgtaattg ttaatttctc tgtcccactc catccaggtc gtgtgattcc
      601 aaatctgttc cagagattta ttactccaac tagcattcca aggcacagca gtggtgcaaa
      661 tgagttttcc agagcaaccc caaatcccca ggagctgttg atcctttagg tatctttcca
      721 cagccaggat tcttgcctgg agctgcttga tgccccagac tgtgagttgc aacagatgct
      781 gttgcgcctc aatagccctc agcaaattgt tctgctgctg cactatacca gacaataatt
      841 gtctggcctg taccgtcagc gtcattgacg ctgcgcccat agtgcttcct gctgctccca
      901 agaacccaag gaacaaagct cc
//
