LOCUS       sequence-56690-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  destination vector for the Golden Gate TALEN kit, for in vitro 
            synthesis of TALEN mRNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Carlson DF, Tan W, Lillico SG, Stverakova D, Proudfoot C, Christian 
            M, Voytas DF, Long CR, Whitelaw CB, Fahrenkrug SC
  TITLE     Efficient TALEN-mediated gene knockout in livestock.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Oct 1.
  PUBMED    23027955
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2012 Oct 1."
COMMENT     Sequence Label: RCIscript-GoldyTALEN
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..436)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGARLQGG
                     TRQCIARCMHCLHCGHAATFCNLHKLACVQL"
     primer_bind     286..308
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     300..317
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     301..317
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     327..346
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        327..345
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(393..413)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(393..411)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(432..448)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(432..448)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(445..467)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    456..472
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(480..486,487..504,505..510))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    525..546
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ggatgcgggg gagttgagag gtccgccgtt acagttggac acaggccaac ttgtgaagat
       61 tgcaaaacgt ggcggcgtga ccgcaatgga ggcagtgcat gcatcgcgca atgcactgac
      121 gggtgccccc ctggagacgg gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc
      181 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
      241 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
      301 gtaaaacgac ggccagtgag cgcgcgtaat acgactcact atagggcgaa ttgggtaccg
      361 ggccccccct cgaggtcctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg
      421 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
      481 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
      541 acattaattg cgttgcgctc actgcccgct ttccaccggt cgtctccaac gaccacctcg
      601 tcgccttggc ctgcctcggc ggacgtcctg ccatggatgc agtgaaaaag ggattgccgc
      661 acgcgccgga attgatcaga agagtcaatc gccgtattgg cgaacgcacg tcccatcgcg
      721 ttgcctctag atcccagcta gtgaaatctg aattggaaga gaagaaatct gaacttagac
      781 ataaattgaa atatgtgcca catgaatata ttgaattgat tgaaatcgca
//
