LOCUS       sequence-56691-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  destination vector for the Golden Gate TALEN kit, for in vitro 
            synthesis of TALEN mRNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Carlson DF, Tan W, Lillico SG, Stverakova D, Proudfoot C, Christian 
            M, Voytas DF, Long CR, Whitelaw CB, Fahrenkrug SC
  TITLE     Efficient TALEN-mediated gene knockout in livestock.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Oct 1.
  PUBMED    23027955
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2012 Oct 1."
COMMENT     Sequence Label: RCIscript-GoldyTALEN
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     206..223
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        254..272
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     5'UTR           281..323
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     CDS             393..413
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             414..440
                     /codon_start=1
                     /product="epitope tag from the baculovirus Autographa 
                     californica GP64 envelope fusion protein"
                     /label=AcV5 tag
                     /translation="SWKDASGWS"
ORIGIN
        1 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
       61 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
      121 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
      181 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
      241 aatacgcaaa ccgaattaac cctcactaaa gggaagcttg cttgttcttt ttgcagaagc
      301 tcagaataaa cgctcaactt tggcagatct aactcgagaa agatattgta tatatcgtaa
      361 caataggagg ttcaacaatg gcttcctccc ctccaaagaa aaagagaaag gttagttgga
      421 aggacgcaag tggttggtct agagtggatc tacgcacgct cggctacagt cagcagcagc
      481 aagagaagat caaaccgaag gtgcgttcga cagtggcgca gcaccacgag gcactggtgg
      541 gccatgggtt tacacacgcg cacatcgttg cgctcagcca acacccggca gcgttaggga
      601 ccgtcgctgt cacgtatcag cacataatca cggcgttgcc agaggcgaca cacgaagaca
      661 tcgttggcgt cggcaaacag tggtccggcg cacgcgccct ggaggccttg ctcacggatg
      721 cgggggagtt gagaggtccg ccgttacagt tggacacagg ccaacttgtg aagattgcaa
      781 aacgtggcgg cgtgaccgca atggaggcag tgcatgcatc gcgcaatgca ctgacgggtg
      841 cccccctgga gacgggcgcc
//
