LOCUS       sequence-56698-e        9455 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast expression of VMA1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9455)
  AUTHORS   Hughes AL, Gottschling DE
  TITLE     An early age increase in vacuolar pH limits mitochondrial function 
            and lifespan in yeast.
  JOURNAL   Nature. 2012 Dec 13;492(7428):261-5. doi: 10.1038/nature11654. Epub 
            2012 Nov 21.
  PUBMED    23172144
REFERENCE   2  (bases 1 to 9455)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature11654"; journalName: "Nature"; date: "2012-12-13- 
            13"; volume: "492"; issue: "7428"; pages: "261-5"
COMMENT     Sequence Label: pAG306-GPD-VMA1 chr I
FEATURES             Location/Qualifiers
     source          1..9455
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        190..410
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             411..1214
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(1343..1798)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1480..1501)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1692..1711
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1928..1950
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1942..1959
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1943..1959
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1966..1985
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1966..1984
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        2009..2653
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     2547..2570
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     primer_bind     2659..2675
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2659..2675
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    2683..2707
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3571..4389
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae VMA1"
                     /product="5' region of the intein from the yeast Vma1 
                     subunit of the vacuolar ATPase (Chong et al., 1998)"
                     /label=Sce VMA intein 5' region
                     /translation="CFAKGTNVLMADGSIECIENIEVGNKVMGKDGRPREVIKLPRGRE
                     TMYSVVQKSQHRAHKSDSSREVPELLKFTCNATHELVVRTPRSVRRLSRTIKGVEYFEV
                     ITFEMGQKKAPDGRIVELVKEVSKSYPISEGPERANELVESYRKASNKAYFEWTIEARD
                     LSLLGSHVRKATYQTYAPILYENDHFFDYMQKSKFHLTIEGPKVLAYLLGLWIGDGLSD
                     RATFSVDSRDTSLMERVTEYAEKLNLCAEYKDRKEPQVAKTVNLYSKVVRG"
     CDS             4845..4856
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(5939..5963)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5996..6012)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5997..6013)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      6015..6262
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(6031..6049)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     promoter        complement(6285..6303)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6324..6340)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6324..6340)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6337..6359)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6348..6364
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6372..6378,6379..6396,6397..6402))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(6555..6572)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6726..7314)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(7485..8276,8277..8345))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8108..8127
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8346..8450)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9428..9446
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accacgcttt tcaattcaat tcatcatttt ttttttattc ttttttttga tttcggtttc
      241 tttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca
      301 gacttagatt ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat
      361 tcttaaccca actgcacaga acaaaaacct gcaggaaacg aagataaatc atgtcgaaag
      421 ctacatataa ggaacgtgct gctactcatc ctagtcctgt tgctgccaag ctatttaata
      481 tcatgcacga aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc accaaggaat
      541 tactggagtt agttgaagca ttaggtccca aaatttgttt actaaaaaca catgtggata
      601 tcttgactga tttttccatg gagggcacag ttaagccgct aaaggcatta tccgccaagt
      661 acaatttttt actcttcgaa gacagaaaat ttgctgacat tggtaataca gtcaaattgc
      721 agtactctgc gggtgtatac agaatagcag aatgggcaga cattacgaat gcacacggtg
      781 tggtgggccc aggtattgtt agcggtttga agcaggcggc agaagaagta acaaaggaac
      841 ctagaggcct tttgatgtta gcagaattgt catgcaaggg ctccctatct actggagaat
      901 atactaaggg tactgttgac attgcgaaga gcgacaaaga ttttgttatc ggctttattg
      961 ctcaaagaga catgggtgga agagatgaag gttacgattg gttgattatg acacccggtg
     1021 tgggtttaga tgacaaggga gacgcattgg gtcaacagta tagaaccgtg gatgatgtgg
     1081 tctctacagg atctgacatt attattgttg gaagaggact atttgcaaag ggaagggatg
     1141 ctaaggtaga gggtgaacgt tacagaaaag caggctggga agcatatttg agaagatgcg
     1201 gccagcaaaa ctaaaaaact gtattataag taaatgcatg tatactaaac tcacaaatta
     1261 gagcttcaat ttaattatat cagttattac cctgcggtgt gaaataccgc acagatgcgt
     1321 aaggagaaaa taccgcatca ggaaattgta aacgttaata ttttgttaaa attcgcgtta
     1381 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     1441 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     1501 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     1561 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     1621 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     1681 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     1741 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg
     1801 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     1861 ctattacgcc agctggcgaa ggggggatgt gctgcaaggc gattaagttg ggtaacgcca
     1921 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta
     1981 tagggcgaat tggagctctt cgagtttatc attatcaata ctcgccattt caaagaatac
     2041 gtaaataatt aatagtagtg attttcctaa ctttatttag tcaaaaaatt agccttttaa
     2101 ttctgctgta acccgtacat gcccaaaata gggggcgggt tacacagaat atataacacc
     2161 gaaggtgcct gggtgaacag gtttattcct ggcatccact aaatataatg gagcccgctt
     2221 tttaagctgg catccagaaa aaaaaagaat cccagcacca aaatattgtt ttcttcacca
     2281 accatcagtt cataggtcca ttctcttagc gcaactacag agaacagggg cacaaacagg
     2341 caaaaaacgg gcacaacctc aatggagtga tgcaacctgc ctggagtaaa tgatgacaca
     2401 aggcaattga cccacgcatg tatctatctc attttcttac accttctatt accttctgct
     2461 ctctctgatt tggaaaaagc tgaaaaaaaa ggttgaaacc agttccctga aattattccc
     2521 ctacttgact aataagtata taaagacggt aggtattgat tgtaattctg taaatctatt
     2581 tcttaaactt cttaaattct acttttatag ttagtctttt ttttagtttt aaaacaccag
     2641 aacttagttt cgacggattc tagaactagt ggatccccca tcacaagttt gtacaaaaaa
     2701 gcaggcttcc agctgaccac catggctggt gcaattgaaa acgctcgtaa ggaaataaaa
     2761 agaatctcat tagaagacca tgctgaatct gaatatggtg ccatctattc tgtctctggt
     2821 ccggtcgtca ttgctgaaaa tatgattggt tgtgccatgt acgaattggt caaggtcggt
     2881 cacgataacc tggtgggtga agtcattaga attgacggtg acaaggccac catccaagtt
     2941 tacgaagaaa ctgcaggcct tacggtcggt gaccctgttt cgagaacagg taagcctctg
     3001 tcggtagaat tgggtcctgg tctgatggaa accatttacg atggtattca aagacctttg
     3061 aaggccatta aggaagaatc gcaatcgatt tatatcccaa gaggtattga cactccagct
     3121 ttggatagga ctatcaagtg gcaatttact ccgggaaagt ttcaagtcgg cgatcatatt
     3181 tccggtggtg atatttacgg ttccgttttt gagaattcgc taatttcaag ccataagatt
     3241 cttttgccac caagatcaag aggtacaatc acttggattg ctccagctgg tgagtacact
     3301 ttggatgaga agattttgga agttgaattt gatggcaaga agtctgattt cactctttac
     3361 catacttggc ctgttcgtgt tccaagacca gttactgaaa agttatctgc tgactatcct
     3421 ttgttaacag gtcaaagagt tttggatgct ttgtttcctt gtgttcaagg tggtacgaca
     3481 tgtattccag gtgcttttgg ttgtggtaag accgttatct ctcaatcttt gtccaagtac
     3541 tccaattctg acgccattat ctatgtcggg tgctttgcca agggtaccaa tgttttaatg
     3601 gcggatgggt ctattgaatg tattgaaaac attgaggttg gtaataaggt catgggtaaa
     3661 gatggcagac ctcgtgaggt aattaaattg cccagaggaa gagaaactat gtacagcgtc
     3721 gtgcagaaaa gtcagcacag agcccacaaa agtgactcaa gtcgtgaagt gccagaatta
     3781 ctcaagttta cgtgtaatgc gacccatgag ttggttgtta gaacacctcg tagtgtccgc
     3841 cgtttgtctc gtaccattaa gggtgtcgaa tattttgaag ttattacttt tgagatgggc
     3901 caaaagaaag cccccgacgg tagaattgtt gagcttgtca aggaagtttc aaagagctac
     3961 ccaatatctg aggggcctga gagagccaac gaattagtag aatcctatag aaaggcttca
     4021 aataaagctt attttgagtg gactattgag gccagagatc tttctctgtt gggttcccat
     4081 gttcgtaaag ctacctacca gacttacgct ccaattcttt atgagaatga ccactttttc
     4141 gactacatgc aaaaaagtaa gtttcatctc accattgaag gtccaaaagt acttgcttat
     4201 ttacttggtt tatggattgg tgatggattg tctgacaggg caactttttc ggttgattcc
     4261 agagatactt ctttgatgga acgtgttact gaatatgctg aaaagttgaa tttgtgcgcc
     4321 gagtataagg acagaaaaga accacaagtt gccaaaactg ttaatttgta ctctaaagtt
     4381 gtcagaggta atggtattcg caataatctt aatactgaga atccattatg ggacgctatt
     4441 gttggcttag gattcttgaa ggacggtgtc aaaaatattc cttctttctt gtctacggac
     4501 aatatcggta ctcgtgaaac atttcttgct ggtctaattg attctgatgg ctatgttact
     4561 gatgagcatg gtattaaagc aacaataaag acaattcata cttctgtcag agatggtttg
     4621 gtttcccttg ctcgttcttt aggcttagta gtctcggtta acgcagaacc tgctaaggtt
     4681 gacatgaatg gcaccaaaca taaaattagt tatgctattt atatgtctgg tggagatgtt
     4741 ttgcttaacg ttctttcgaa gtgtgccggc tctaaaaaat tcaggcctgc tcccgccgct
     4801 gcttttgcac gtgagtgccg cggattttat ttcgagttac aagaattgaa ggaagacgat
     4861 tattgtggga ttactttatc tgatgattct gatcatcagt ttttgcttgc caaccaggtt
     4921 gtcgtccata attgcggaga aagaggtaat gaaatggcag aagtcttgat ggaattccca
     4981 gagttatata ctgaaatgag cggtactaaa gaaccaatta tgaagcgtac tactttggtc
     5041 gctaatacat ctaacatgcc ggttgcagcc agagaagctt ctatttacac tggtatcact
     5101 cttgcagaat acttcagaga tcaaggtaaa aatgtttcta tgattgcaga ctcttcttca
     5161 agatgggctg aagctttgag agaaatttct ggtcgtttgg gtgagatgcc tgctgatcaa
     5221 ggtttcccag cttatttggg tgctaagttg gcctcctttt acgaaagagc cggtaaagct
     5281 gttgctttag gttccccaga tcgtactggt tccgtttcca tcgttgctgc cgtttcgcca
     5341 gccggtggtg atttctcaga tcctgttact actgctacat tgggtatcac tcaagtcttt
     5401 tggggtttag acaagaaatt ggctcaaaga aagcatttcc catctatcaa cacatctgtt
     5461 tcttactcca aatacactaa tgtcttgaac aagttttatg attccaatta ccctgaattt
     5521 cctgttttaa gagatcgtat gaaggaaatt ctatcaaacg ctgaagaatt agaacaagtt
     5581 gttcaattag ttggtaaatc ggccttgtct gatagtgata agattacttt ggatgttgcc
     5641 actttaatca aggaagattt cttgcaacaa aatggttact ccacttatga tgctttctgt
     5701 ccaatttgga agacatttga tatgatgaga gccttcatct cgtatcatga cgaagctcaa
     5761 aaagctgttg ctaatggtgc caactggtca aaactagctg actctactgg tgacgttaag
     5821 catgccgttt cttcatctaa attttttgaa ccaagcaggg gtgaaaagga agtccatggc
     5881 gaattcgaaa aattgttgag cactatgcaa gaaagatttg ctgaatctac cgattaagac
     5941 ccagctttct tgtacaaagt ggtgatgggc tgcaggaatt cgatatcaag cttatcgata
     6001 ccgtcgacct cgagtcatgt aattagttat gtcacgctta cattcacgcc ctccccccac
     6061 atccgctcta accgaaaagg aaggagttag acaacctgaa gtctaggtcc ctatttattt
     6121 ttttatagtt atgttagtat taagaacgtt atttatattt caaatttttc ttttttttct
     6181 gtacagacgc gtgtacgcat gtaacattat actgaaaacc ttgcttgaga aggttttggg
     6241 acgctcgaag gctttaattt gcggccggta cccagctttt gttcccttta gtgagggtta
     6301 attccgagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     6361 acaattccac acaacatagg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     6421 gtgaggtaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     6481 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     6541 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     6601 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     6661 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     6721 gcgtttttcc ataggctcgg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     6781 aggtggcgaa acccgacagg actataaaga taccaggcgt tcccccctgg aagctccctc
     6841 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     6901 ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     6961 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     7021 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     7081 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     7141 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     7201 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     7261 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     7321 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     7381 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     7441 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     7501 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactgccc
     7561 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     7621 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     7681 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     7741 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     7801 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     7861 cgatcaaggc gagttacatg atcccccatg ttgtgaaaaa aagcggttag ctccttcggt
     7921 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     7981 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     8041 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     8101 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     8161 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     8221 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     8281 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     8341 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     8401 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     8461 cgaaaagtgc cacctggggg gtgctaatta tggcattgat tggggcggta aaacattcaa
     8521 agtcaatgaa aaggatgcta aattgaatga tactgaagac atttcattcg cttctcagat
     8581 caaaaactta aatctgtcta tatgagctgt cccaaagcca cttcttacct ttcaagaaaa
     8641 catggacctt gctttgccac aaaataattg cctgtaagat agttttttat ttgcacattg
     8701 ttattactaa cctatgcttt gctacggacg actgcttggt aaaaatgcaa aagtattagg
     8761 atgtctcaca agaaactgct cgtggtgaaa attttcagcc gcgaccatcc tcccacgcca
     8821 acaagccctc ttttgcccat ccttctggaa ttcgcggtga aaatcaattt gatatataaa
     8881 attccctcat aatatctagg aacgggatca catatctcaa tgagctaaag cggccgcttc
     8941 ttcgaatggg accagctaaa gtgatcaaac ttcatgcagg atgttggaca ctttttcttt
     9001 caagaagctc caaatatata aatttttgca atataggaat tgaaatatcg tacttccgat
     9061 tcagaatatc tcaactctac tacatctata tatgactgta ctcgcgttct tggtaaacat
     9121 caattagtag gtgtttttgc gagcatatgt tgagctcaga aaatatgagc acaatcttcg
     9181 aaaccattgt ttttttgtgc aaaagtcacc atcgggttta tagtatggac tcttgaacga
     9241 accattctga tctgtatttc tctcatattt actccattta ttttttgctg ctctttgctt
     9301 tacttcaaca cgatgctttt cctaagtaat tttttatttt attcacaatg ctccataact
     9361 cttcaggcct taaacgacca tccatggccc ctaagaaacc attattatca tgacattaac
     9421 ctataaaaat aggcgtatca cgaggccctt tcgtc
//
