LOCUS       sequence-56710-e         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral Gateway destination vector. Puromycin 
            selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Yang X, Boehm JS, Yang X, Salehi-Ashtiani K, Hao T, Shen Y, Lubonja 
            R, Thomas SR, Alkan O, Bhimdi T, Green TM, Johannessen CM, Silver 
            SJ, Nguyen C, Murray RR, Hieronymus H, Balcha D, Fan C, Lin C, 
            Ghamsari L, Vidal M, Hahn WC, Hill DE, Root DE
  TITLE     A public genome-scale lentiviral expression library of human ORFs.
  JOURNAL   Nat Methods. 2011 Jun 26;8(8):659-61. doi: 10.1038/nmeth.1638
  PUBMED    21706014
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-06-26- 26"; volume: "8"; 
            issue: "8"; pages: "659-61"
COMMENT     Sequence Label: pLX301
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(120..244)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    287..875
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(340..360)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(758..769)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct
       61 gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc
      121 atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct
      181 aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag
      241 tggtttagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga
      301 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
      361 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
      421 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
      481 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
      541 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
      601 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
      661 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
      721 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
      781 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
      841 gacgagtcgg atctcccttt gggccgcctc cccgcgtcga ct
//
