LOCUS       sequence-56711-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral Gateway destination vector. Puromycin 
            selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Yang X, Boehm JS, Yang X, Salehi-Ashtiani K, Hao T, Shen Y, Lubonja 
            R, Thomas SR, Alkan O, Bhimdi T, Green TM, Johannessen CM, Silver 
            SJ, Nguyen C, Murray RR, Hieronymus H, Balcha D, Fan C, Lin C, 
            Ghamsari L, Vidal M, Hahn WC, Hill DE, Root DE
  TITLE     A public genome-scale lentiviral expression library of human ORFs.
  JOURNAL   Nat Methods. 2011 Jun 26;8(8):659-61. doi: 10.1038/nmeth.1638
  PUBMED    21706014
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-06-26- 26"; volume: "8"; 
            issue: "8"; pages: "659-61"
COMMENT     Sequence Label: pLX301
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(61..79)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             complement(673..717)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gggagagagg tcggtgattc ggtcaacgag ggagccgact gccgacgtgc gctccggagg
       61 cttgcagaat gcggaacacc gcgcgggcag gaacagggcc cacactaccg ccccacaccc
      121 cgcctcccgc accgcccctt cccggccgct gctctcggcg cgccctgctg agcagccgct
      181 attggccaca gcccatcgcg gtcggcgcgc tgccattgct ccctggcgct gtccgtctgc
      241 gagggtacta gtgagacgtg cggcttccgt ttgtcacgtc cggcacgccg cgaaccgcaa
      301 ggaaccttcc cgacttaggg gcggagcagg aagcgtcgcc ggggggccca caagggtagc
      361 ggcgaagatc cgggtgacgc tgcgaacgga cgtgaagaat gtgcgagacc cagggtcggc
      421 gccgctgcgt ttcccggaac cacgcccaga gcagccgcgt ccctgcgcaa acccagggct
      481 gccttggaaa aggcgcaagg gtcccctcgg ggttgggagg tgggtctgaa acgataatgg
      541 tgaatatccc tgcctaactc tattcactat agaaagtaca gcaaaaacta ttcttaaacc
      601 taccaagcct cctactatca ttatgaataa ttttatatac cacagccaat ttgttatgtt
      661 aaaccaattc cacaaacttg cccatttatc taattccaat aattcttgtt cattcttttc
      721 ttgctggttt tgcgattctt caattaagga gtgtattaag cttgtgtaat tgttaatttc
      781 tctgtcccac tccatccagg tcgtgtgatt ccaaatctgt tccagagatt tattactcca
      841 actagcattc caaggcacag cagtggtgca aatgagtttt
//
