LOCUS       sequence-56769-e         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller 
            JA, Somia NV, Bogdanove AJ, Voytas DF
  TITLE     Efficient design and assembly of custom TALEN and other TAL 
            effector-based constructs for DNA targeting.
  JOURNAL   Nucleic Acids Res. 2011 Jul;39(12):e82. doi: 10.1093/nar/gkr218
  PUBMED    21493687
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-07"; volume: "39(12):e82. doi"; pages: " 
            10.1093/nar/gkr21"
COMMENT     Sequence Label: pCP5b
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     163..180
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     164..180
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    197..428
                     /gene="mutant version of attP"
                     /label=attP1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(926..945)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 tataagtaaa tgcatgtata ctaaactcac aaattagagc ttcaatttaa ttatatcagt
       61 tattacccta tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
      121 aaattgtaag cgttaatggg tttcatggta tcagagatct gttgtaaaac gacggccagt
      181 cttaagctcg ggccccaaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa
      241 caaattgatg agcaatgctt ttttataatg ccaactttgt acaaaaaagc tgaacgagaa
      301 acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa acagactaca
      361 taatactgta aaacacaaca tatccagtca ctatgaatca actacttaga tggtattagt
      421 gacctgtagt cgaccgacag ccttccaaat gttcttcggg tgatgctgcc aacttagtcg
      481 accgacagcc ttccaaatgt tcttctcaaa cggaatcgtc gtatccagcc tactcgctat
      541 tgtcctcaat gccgtattaa atcataaaaa gaaataagaa aaagaggtgc gagcctcttt
      601 tttgtgtgac aaaataaaaa catctaccta ttcatatacg ctagtgtcat agtcctgaaa
      661 atcatctgca tcaagaacaa tttcacaact cttatacttt tctcttacaa gtcgttcggc
      721 ttcatctgga ttttcagcct ctatacttac taaacgtgat aaagtttctg taatttctac
      781 tgtatcgacc tgcagactgg ctgtgtataa gggagcctga catttatatt ccccagaaca
      841 tcaggttaat ggcgtttttg atgtcatttt cgcggtggct gagatcagcc acttcttccc
      901 cgataacgga gaccggcaca ctggccatat cggtggtcat catgcgccag ctttcatccc
      961 cgatatgca
//
