LOCUS       sequence-56772-e         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Zhang Y, Zhang F, Li X, Baller JA, Qi Y, Starker CG, Bogdanove AJ, 
            Voytas DF
  TITLE     TALENs enable efficient plant genome engineering.
  JOURNAL   Plant Physiol. 2012 Nov 2.
  PUBMED    23124327
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2012 Nov 2."
COMMENT     Sequence Label: pZHY500
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..26
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7..25
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(73..93)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(73..91)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(112..128)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(112..128)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(125..147)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    136..152
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(160..166,167..184,185..190))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    205..226
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cgcgcgtaat acgactcact atagggcgaa ttgggtaccg ggccccccct cgaggtcctc
       61 cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct
      121 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
      181 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
      241 actgcccgct ttccaccggt cgtctccaac gaccacctcg tcgccttggc ctgcctcggc
      301 ggacgtcctg ccatggatgc agtgaaaaag ggattgccgc acgcgccgga attgatcaga
      361 agagtcaatc gccgtattgg cgaacgcacg tcccatcgcg ttgccggatc ccagctagtg
      421 aaatctgaat tggaagagaa gaaatctgaa cttagacata aattgaaata tgtgccacat
      481 gaatatattg aattgattga aatcgcaaga aattcaactc aggatagaat ccttgaaatg
      541 aaggtgatgg agttctttat gaaggtttat ggttatcgtg gtaaacattt gggtggatca
      601 aggaaaccag acggagcaat ttatactgtc ggatctccta ttgattacgg tgtgatcgtt
      661 gatactaagg catattcagg aggttataat cttccaattg gtcaagcaga tgaaatgcaa
      721 agatatgtcg aagagaatca aacaagaaac aagcatatca accctaatga atggtggaaa
      781 gtctatccat cttcagtaac agaatttaag ttcttgtttg tgagtggtca tttcaaagga
      841 aactacaaag ctcagcttac aagattgaat catatcacta attgtaatgg agctgttctt
      901 agtgtagaag agcttttgat tggtggagaa atgattaaag ctggtacatt gacacttgag
      961 gaagtgagaa g
//
