LOCUS       sequence-56774-e         952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Zhang Y, Zhang F, Li X, Baller JA, Qi Y, Starker CG, Bogdanove AJ, 
            Voytas DF
  TITLE     TALENs enable efficient plant genome engineering.
  JOURNAL   Plant Physiol. 2012 Nov 2.
  PUBMED    23124327
REFERENCE   2  (bases 1 to 952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2012 Nov 2."
COMMENT     Sequence Label: pZHY501
FEATURES             Location/Qualifiers
     source          1..952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    738..759
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(774..779,780..797,798..804)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    812..828
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     817..839
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     836..852
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     836..852
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     871..891
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        873..891
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttcctcaagt gtcaatgtac cagctttaat catttctcca ccaatcaaaa gctcttctac
       61 actaagaaca gctccattac aattagtgat atgattcaat cttgtaagct gagctttgta
      121 gtttcctttg aaatgaccac tcacaaacaa gaacttaaat tctgttactg aagatggata
      181 gactttccac cattcattag ggttgatatg cttgtttctt gtttgattct cttcgacata
      241 tctttgcatt tcatctgctt gaccaattgg aagattataa cctcctgaat atgccttagt
      301 atcaacgatc acaccgtaat caataggaga tccgacagta taaattgctc cgtctggttt
      361 ccttgatcca cccaaatgtt taccacgata accataaacc ttcataaaga actccatcac
      421 cttcatttca aggattctat cctgagttga atttcttgcg atttcaatca attcaatata
      481 ttcatgtggc acatatttca atttatgtct aagttcagat ttcttctctt ccaattcaga
      541 tttcactagc tgggatccgg caacgcgatg ggacgtgcgt tcgccaatac ggcgattgac
      601 tcttctgatc aattccggcg cgtgcggcaa tccctttttc actgcatcca tggcaggacg
      661 tccgccgagg caggccaagg cgacgaggtg gtcgttggag acgaccggtg gaaagcgggc
      721 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
      781 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
      841 aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag
      901 ctggaggacc tcgagggggg gcccggtacc caattcgccc tatagtgagt cg
//
