LOCUS       sequence-56786-e         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses caged Cdc42 GEF intersectin: Intersectin Dbl homology (DH)
            domain fused to Dronpa145N at C terminus and at N terminus, for 
            optogenetic activation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Zhou XX, Chung HK, Lam AJ, Lin MZ
  TITLE     Optical control of protein activity by fluorescent protein domains.
  JOURNAL   Science. 2012 Nov 9;338(6108):810-4. doi: 10.1126/science.1226854.
  PUBMED    23139335
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2012-11-9- 9"; volume: "338"; issue: 
            "6108"; pages: "810-4"
COMMENT     Sequence Label: pcDNA3-N-I-N-CAAX
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     34..53
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        34..52
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tagagaaccc actgcttact ggcttatcga aattaatacg actcactata gggagaccca
       61 agcttatgag cgtgatcaag cccgacatga aaatcaagct gaggatggag ggtgccgtga
      121 acggtcaccc gtttgcgatc gagggcgtgg gactgggcaa gccgttcgag ggaaagcaaa
      181 gcatggacct caaggtgaag gagggcggac cgctgccctt cgcctacgac attctgacca
      241 ctgtcttctg ctacggtaat cgcgtcttcg caaagtatcc cgaaaacatc gtggattact
      301 ttaagcagag cttcccggag ggttactcct gggagcgatc catgaactac gaggacggag
      361 ggatctgcaa cgcgacgaac gatataacct tggacggcga ctgctacatt tacgaaattc
      421 ggttcgacgg cgtgaatttc ccagcgaatg gccctgtaat gcaaaaacgc acggtaaagt
      481 gggagcccag caccgagaac ctgtacgtcc gcgacggagt cctgaaggga gacgtgaaca
      541 tggcgctttc attggaaggt ggcgggcact acagatgcga tttcaagacg acctataagg
      601 cgaaaaaggt ggtccaactg ccggactacc atttcgtcga ccaccacatc gagatcaaga
      661 gccacgacaa agactatagc aacgtgaatc tccacgagca tgccgaggcc cacagcgagc
      721 tgcccaggca ggccaagggc cttaagggat ccatgactga aaggaagagg cagggctaca
      781 tccacgagct gattgtcacg gaagagaatt acg
//
