LOCUS       sequence-56788-e       11239 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11239)
  AUTHORS   Nishi K, Nishi A, Nagasawa T, Ui-Tei K
  TITLE     Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated
            gene silencing in the nucleus.
  JOURNAL   RNA. 2013 Jan;19(1):17-35. doi: 10.1261/rna.034769.112. Epub 2012 
            Nov 13.
  PUBMED    23150874
REFERENCE   2  (bases 1 to 11239)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.034769.112"; journalName: "RNA"; date: "2013-01"; 
            volume: "19"; issue: "1"; pages: "17-35"
COMMENT     Sequence Label: pmyc-GFP-TNRC6A-NLS-mut
FEATURES             Location/Qualifiers
     source          1..11239
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             910..939
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(949..951,952..954,955..1665)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(949..951,952..954,955..1665)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(994..1015)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1255..1274)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1602..1623
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    5886..5897
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     primer_bind     complement(6833..6850)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6839..7063
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7109..7537
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7196..7215)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7406..7427
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7546..7566)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7551..7880
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7731..7866
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7793..7812
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7947..8741
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8001..8020)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8611..8630
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8915..9036
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8952..8971)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9006..9025
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9085..9101)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9085..9101)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9098..9120)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9109..9125
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9133..9139,9140..9157,9158..9163))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9178..9199
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9316..9333)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9487..10072)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9567..9586)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10243..11034,11035..11103))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10866..10885
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11104..11208)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 ccaccaatgg aacaaaaact catctcagaa gaggatctgt ccggcggcat ggtgagcaag
      961 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     1021 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     1081 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     1141 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     1201 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     1261 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1321 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1381 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     1441 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1501 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     1561 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1621 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagaagct tatggatgct
     1681 gattctgcct ccagttctga atcagagaga aacatcacta tcatggcttc agggaacaca
     1741 ggtggtgaaa aagatggcct tcggaatagc actggacttg gttcccaaaa caagtttgta
     1801 gttggtagca gcagcaataa tgtgggccat ggaagtagta ctgggccatg gggtttttcc
     1861 catggagcca taataagcac atgtcaggtc tctgtggatg ctcctgaaag caaatctgaa
     1921 agtagcaaca atagaatgaa tgcttggggc actgtaagtt cttcatcaaa tggagggtta
     1981 aatccaagca ctttgaattc agctagcaac catggtgcct ggccagtatt agagaacaat
     2041 ggacttgccc taaaagggcc tgtagggagt ggtagttctg gcattaatat tcagtgcagt
     2101 actataggcc agatgcctaa caatcagagt attaactcta aagtgagtgg tggttctacc
     2161 catggtacct ggggaagcct tcaggaaact tgtgaatctg aagtaagtgg tacacagaag
     2221 gtttcattca gtggtcaacc tcaaaatatt accactgaaa tgactggacc aaataacact
     2281 actaacttta tgacctctag tttaccaaac tccggttcag tgcagaataa tgagctgcct
     2341 agtagtaaca caggggcctg gcgtgtgagc acaatgaatc atcctcagat gcaggctcca
     2401 tcaggtatga atggcacttc cctttctcac cttagcaatg gagagtcaaa aagtggaggc
     2461 tcttatggta ctacatgggg tgcctatggt tctaattact ctggagacaa atgttcaggc
     2521 cctaatggcc aagctaatgg tgacactgtg aatgcaactc taatgcagcc tggcgtaaat
     2581 ggtcctatgg gcactaactt tcaagttaac acaaacaaag gaggtggtgt gtgggaatct
     2641 ggtgcagcaa actcccagag tacatcatgg ggaagtggaa atggcgcaaa ttctggagga
     2701 agtcgaagag gatggggaac ccctgcacaa aacactggca ctaatttacc cagcgttgag
     2761 tggaacaaac tgcctagcaa tcagcattcc aatgatagtg caaatggcaa tggtaagacg
     2821 tttacaaatg gatggaaatc tactgaggaa gaggatcagg gttctgccac atctcagaca
     2881 aatgagcaaa gcagtgtgtg ggccaaaaca ggaggtacag tggagagcga tggtagtaca
     2941 gaaagcactg gacgccttga ggaaaaagga actggggaaa gtcagagtag agacagaaga
     3001 aaaattgatc agcacacatt actccaaagc attgtaaaca gaactgactt agatccacgt
     3061 gtcctgtcca actctggttg gggacagact cctattaagc agaatactgc ctgggataca
     3121 gaaacatcac ctagagggga acgaaagact gacaatggga cagaggcctg gggaagctct
     3181 gcaacacaga cttttaactc aggggcatgt atagataaga ctagccctaa tggtaatgat
     3241 acctcatctg tatcagggtg gggcgatccc aaacctgctc tgaggtgggg agattccaaa
     3301 ggctcaaact gccagggggg gtgggaagat gattctgctg ctacaggaat ggtcaagagc
     3361 aatcagtggg ggaattgcaa agaggagaag gctgcatgga atgactcgca aaagaataaa
     3421 cagggatggg gtgatggaca aaaatcaagc caagggtggt ctgtttctgc cagtgataac
     3481 tggggagaaa cttcaaggaa taaccattgg ggtgaggcca ataagaaatc cagctcagga
     3541 ggtagtgaca gtgacaggtc cgtttccggt tggaacgaac ttggtaaaac tagttctttc
     3601 acttggggaa acaacataaa tccaaataat tcatcaggat gggatgaatc ttctaaacct
     3661 actccttccc agggatgggg agaccctcca aagtctaatc agtctctagg ttggggagat
     3721 tcgtcaaagc cagtcagctc tccagactgg aacaagcaac aagacattgt tggatcttgg
     3781 ggaatcccac cagctacagg caaacctcct ggtacaggct ggctgggggg acctatacca
     3841 gccccagcaa aagaagaaga acccacaggc tgggaggaac catccccaga atctatacgt
     3901 cgcaaaatgg agattgatga tggaacttca gcttggggag atccaagcaa atacaactac
     3961 aaaaatgtga acatgtggaa caaaaacgtc ccaaatggca acagccgttc agaccagcaa
     4021 gcacaggtac atcagctgct aacgcctgca agtgccatct caaacaaaga ggcaagcagt
     4081 ggctctggct ggggtgagcc ctggggggag ccttctactc cagccacaac tgtggataat
     4141 ggtacttcag catggggtaa gcccatagac agtggtccca gctgggggga acccattgct
     4201 gcggcatcca gcacatccac gtggggctcc agctctgttg gtccacaagc attaagcaaa
     4261 tctgggccaa aatctatgca agatggctgg tgtggtgatg atatgccatt gcctggaaat
     4321 cgccccactg gctgggaaga ggaagaggat gtggagattg gaatgtggaa tagtaattca
     4381 tctcaagagc ttaactcatc tttaaattgg ccaccatata caaagaaaat gtcatcgaag
     4441 ggtctgagtg gcaaaaaagc cgctgccgaa gctggaatga tgaaaggtgg aaacaaacaa
     4501 gaagaagcgt ggataaatcc atttgttaaa cagttttcaa acatcagttt ttcgagagac
     4561 tcaccagagg aaaatgtaca aagcaataag atggaccttt ctggaggaat gttacaagac
     4621 aaacgaatgg agatagataa acatagccta aatattggtg attacaatcg aacggtcggg
     4681 aaaggccctg gttctcggcc tcagatttcc aaagagtctt ccatggagcg caatccttat
     4741 tttgataagg atggcattgt agcagatgaa tcccaaaaca tgcagtttat gtccagtcaa
     4801 agcatgaagc ttcccccttc aaatagtgca ctacctaacc aggcccttgg ctccatagca
     4861 gggctgggta tgcaaaactt gaattctgtt agacagaatg gcaatcccag tatgtttggt
     4921 gttggaaaca cagcagcaca accccggggc atgcagcagc ctccagcaca acctcttagt
     4981 tcatctcagc ctaatctccg tgctcaagtg cctcctccat tactctcccc tcaggttcca
     5041 gtttcattgc tgaagtatgc accaaacaac ggtggcctga atccactctt tggccctcaa
     5101 caggtagcca tgctgaacca gctatcccag ctaaaccagc tttctcagat ctcccagtta
     5161 cagcgattgt tagcgcagca gcaaagggcg cagagtcaga gaagcgtgcc ttctgggaac
     5221 cggccgcagc aagaccagca gggtcgacct cttagtgtgc agcagcaaat gatgcaacaa
     5281 tctcgtcaac ttgatccaaa cctgttggtg aagcagcaga ctccaccatc tcagcagcag
     5341 ccactccatc agccagccat gaagtctttc cttgacaatg tcatgcccca cactacacct
     5401 gagctgcaaa aagggccatc accaataaat gctttcagca acttccctat aggcttgaac
     5461 tcaaacttga atgtaaatat ggatatgaac agtattaaag agccacagtc aagactaagg
     5521 aagtggacga cagtggacag catttctgtg aacacatctt tggatcaaaa ctccagcaaa
     5581 catggtgcta tttcaagtgg tttcaggctg gaagagtctc catttgttcc ctatgacttt
     5641 atgaacagca gtacttcacc agccagtcct ccaggttcaa taggagatgg ctggccacgt
     5701 gccaaatcgc ctaacggctc tagcagtgtt aattggccac cagaatttcg tcctggtgag
     5761 ccatggaaag gttatccaaa cattgaccct gaaactgacc cttacgtcac tcctggcagt
     5821 gtcataaaca atctttcaat taatactgtg cgggaagttg accacctcag ggacaggaac
     5881 agtgggtcat cctcatcctt gaacaccacg ctgccttcaa ctagtgcctg gtcatccatt
     5941 cgtgcctcca actacaacgt tcccctcagc agtacagcac aaagcacttc agccagaaat
     6001 agtgattcca aattgacatg gtctcctggt tcagttacaa acacctctct ggctcatgag
     6061 ctgtggaagg tccctttgcc acctaaaaac atcactgctc cgtcccgccc acctccggga
     6121 ctgactggtc agaagccacc cttgtctacg tgggataatt ctccccttcg tataggtgga
     6181 ggatggggaa attctgacgc cagatatacc ccaggttcca gctggggtga gagcagctca
     6241 gggagaataa caaattggct tgttctaaaa aaccttacac ctcagatcga tggctcaact
     6301 ctgcgcactc tgtgcatgca gcacggcccg ctgatcacat tccacctgaa cctccctcac
     6361 ggaaatgctc tggtccgcta cagttcaaaa gaagaggtag tgaaggcaca aaagtctctg
     6421 cacatgtgtg tactggggaa cactactatt cttgctgagt ttgccagtga agaggagatc
     6481 agtcgtttct ttgcacaaag ccagtctctg accccttctc ccggctggca gtctctcggg
     6541 tccagccaga gccggctggg ctccctcgac tgttcccact cattctccag ccggaccgat
     6601 ctcaatcact ggaatggtgc tgggctgtcg ggaactaact gtggagacct tcacggcact
     6661 tcactctggg ggaccccgca ttattccaca agcctgtggg gtcccccaag cagcagcgac
     6721 ccccgaggaa ttagcagccc atctcccatt aacgcttttc tttctgttga ccacctgggt
     6781 gggggtggag agtccatgta atctagaggg cccgtttaaa cccgctgatc agcctcgact
     6841 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     6901 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     6961 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     7021 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     7081 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     7141 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     7201 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     7261 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     7321 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     7381 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     7441 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     7501 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
     7561 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     7621 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     7681 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     7741 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     7801 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     7861 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
     7921 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
     7981 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     8041 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     8101 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
     8161 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     8221 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     8281 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     8341 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     8401 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     8461 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     8521 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     8581 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     8641 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     8701 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
     8761 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
     8821 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     8881 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     8941 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     9001 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     9061 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     9121 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     9181 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     9241 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     9301 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     9361 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     9421 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     9481 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     9541 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     9601 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     9661 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     9721 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     9781 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     9841 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     9901 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     9961 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
    10021 cggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
    10081 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
    10141 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
    10201 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
    10261 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
    10321 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
    10381 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
    10441 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
    10501 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
    10561 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
    10621 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    10681 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    10741 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    10801 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    10861 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    10921 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    10981 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    11041 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    11101 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    11161 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    11221 aaaagtgcca cctgacgtc
//
