LOCUS       sequence-56791-e       11011 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11011)
  AUTHORS   Nishi K, Nishi A, Nagasawa T, Ui-Tei K
  TITLE     Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated
            gene silencing in the nucleus.
  JOURNAL   RNA. 2013 Jan;19(1):17-35. doi: 10.1261/rna.034769.112. Epub 2012 
            Nov 13.
  PUBMED    23150874
REFERENCE   2  (bases 1 to 11011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.034769.112"; journalName: "RNA"; date: "2013-01"; 
            volume: "19"; issue: "1"; pages: "17-35"
COMMENT     Sequence Label: pmyc-GFP-TNRC6A-del.GW-I
FEATURES             Location/Qualifiers
     source          1..11011
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             910..939
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(949..951,952..954,955..1665)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(949..951,952..954,955..1665)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(994..1015)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1255..1274)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1602..1623
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    5658..5669
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     primer_bind     complement(6605..6622)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6611..6835
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6881..7309
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6968..6987)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7178..7199
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7318..7338)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7323..7652
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7503..7638
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7565..7584
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7719..8513
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7773..7792)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8383..8402
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8687..8808
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8724..8743)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8778..8797
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8857..8873)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8857..8873)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8870..8892)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8881..8897
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8905..8911,8912..8929,8930..8935))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8950..8971
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9088..9105)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9259..9844)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9339..9358)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10015..10806,10807..10875))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10638..10657
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10876..10980)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 ccaccaatgg aacaaaaact catctcagaa gaggatctgt ccggcggcat ggtgagcaag
      961 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     1021 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     1081 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     1141 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     1201 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     1261 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1321 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1381 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     1441 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1501 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     1561 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1621 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagaagct tatggatgct
     1681 gattctgcct ccagttctga atcagagaga aacatcacta tcatggcttc agggaacaca
     1741 ggtggtgaaa aagatggcct tcggaatagc actggacttg gttcccaaaa caagtttgta
     1801 gttggtagca gcagcaataa tgtgggccat ggaagtagta ctgggccatg gggtttttcc
     1861 catggagcca taataagcac atgtcaggtc tctgtggatg ctcctgaaag caaatctgaa
     1921 agtagcaaca atagaatgaa tgcttggggc actgtaagtt cttcatcaaa tggagggtta
     1981 aatccaagca ctttgaattc agctagcaac catggtgcct ggccagtatt agagaacaat
     2041 ggacttgccc taaaagggcc tgtagggagt ggtagttctg gcattaatat tcagtgcagt
     2101 actataggcc agatgcctaa caatcagagt attaactcta aagtgagtgg tggttctacc
     2161 catggtacct ggggaagcct tcaggaaact tgtgaatctg aagtaagtgg tacacagaag
     2221 gtttcattca gtggtcaacc tcaaaatatt accactgaaa tgactggacc aaataacact
     2281 actaacttta tgacctctag tttaccaaac tccggttcag tgcagaataa tgagctgcct
     2341 agtagtaaca caggggcctg gcgtgtgagc acaatgaatc atcctcagat gcaggctcca
     2401 tcaggtatga atggcacttc cctttctcac cttagcaatg gagagtcaaa aagtggaggc
     2461 tcttatggta ctacatgggg tgcctatggt tctaattact ctggagacaa atgttcaggc
     2521 cctaatggcc aagctaatgg tgacactgtg aatgcaactc taatgcagcc tggcgtaaat
     2581 ggtcctatgg gcactaactt tcaagttaac acaaacaaag gaggtggtgt gtgggaatct
     2641 ggtgcagcaa actcccagag tacatcatgg ggaagtggaa atggcgcaaa ttctggagga
     2701 agtcgaagag gatggggaac ccctgcacaa aacactggca ctaatttacc cagcgttgag
     2761 tggaacaaac tgcctagcaa tcagcattcc aatgatagtg caaatggcaa tggtaagacg
     2821 tttacaaatg gatggaaatc tactgaggaa gaggatcagg gttctgccac atctcagaca
     2881 aatgagcaaa gcagtgtgtg ggccaaaaca ggaggtacag tggagagcga tggtagtaca
     2941 gaaagcactg gacgccttga ggaaaaagga actggggaaa gtcagagtag agacagaaga
     3001 aaaattgatc agcacacatt actccaaagc attgtaaacc ccaaacctgc tctgaggtgg
     3061 ggagattcca aaggctcaaa ctgccagggg gggtgggaag atgattctgc tgctacagga
     3121 atggtcaaga gcaatcagtg ggggaattgc aaagaggaga aggctgcatg gaatgactcg
     3181 caaaagaata aacagggatg gggtgatgga caaaaatcaa gccaagggtg gtctgtttct
     3241 gccagtgata actggggaga aacttcaagg aataaccatt ggggtgaggc caataagaaa
     3301 tccagctcag gaggtagtga cagtgacagg tccgtttccg gttggaacga acttggtaaa
     3361 actagttctt tcacttgggg aaacaacata aatccaaata attcatcagg atgggatgaa
     3421 tcttctaaac ctactccttc ccagggatgg ggagaccctc caaagtctaa tcagtctcta
     3481 ggttggggag attcgtcaaa gccagtcagc tctccagact ggaacaagca acaagacatt
     3541 gttggatctt ggggaatccc accagctaca ggcaaacctc ctggtacagg ctggctgggg
     3601 ggacctatac cagccccagc aaaagaagaa gaacccacag gctgggagga accatcccca
     3661 gaatctatac gtcgcaaaat ggagattgat gatggaactt cagcttgggg agatccaagc
     3721 aaatacaact acaaaaatgt gaacatgtgg aacaaaaacg tcccaaatgg caacagccgt
     3781 tcagaccagc aagcacaggt acatcagctg ctaacgcctg caagtgccat ctcaaacaaa
     3841 gaggcaagca gtggctctgg ctggggtgag ccctgggggg agccttctac tccagccaca
     3901 actgtggata atggtacttc agcatggggt aagcccatag acagtggtcc cagctggggg
     3961 gaacccattg ctgcggcatc cagcacatcc acgtggggct ccagctctgt tggtccacaa
     4021 gcattaagca aatctgggcc aaaatctatg caagatggct ggtgtggtga tgatatgcca
     4081 ttgcctggaa atcgccccac tggctgggaa gaggaagagg atgtggagat tggaatgtgg
     4141 aatagtaatt catctcaaga gcttaactca tctttaaatt ggccaccata tacaaagaaa
     4201 atgtcatcga agggtctgag tggcaaaaaa aggagaaggg aaaggggaat gatgaaaggt
     4261 ggaaacaaac aagaagaagc gtggataaat ccatttgtta aacagttttc aaacatcagt
     4321 ttttcgagag actcaccaga ggaaaatgta caaagcaata agatggacct ttctggagga
     4381 atgttacaag acaaacgaat ggagatagat aaacatagcc taaatattgg tgattacaat
     4441 cgaacggtcg ggaaaggccc tggttctcgg cctcagattt ccaaagagtc ttccatggag
     4501 cgcaatcctt attttgataa ggatggcatt gtagcagatg aatcccaaaa catgcagttt
     4561 atgtccagtc aaagcatgaa gcttccccct tcaaatagtg cactacctaa ccaggccctt
     4621 ggctccatag cagggctggg tatgcaaaac ttgaattctg ttagacagaa tggcaatccc
     4681 agtatgtttg gtgttggaaa cacagcagca caaccccggg gcatgcagca gcctccagca
     4741 caacctctta gttcatctca gcctaatctc cgtgctcaag tgcctcctcc attactctcc
     4801 cctcaggttc cagtttcatt gctgaagtat gcaccaaaca acggtggcct gaatccactc
     4861 tttggccctc aacaggtagc catgctgaac cagctatccc agctaaacca gctttctcag
     4921 atctcccagt tacagcgatt gttagcgcag cagcaaaggg cgcagagtca gagaagcgtg
     4981 ccttctggga accggccgca gcaagaccag cagggtcgac ctcttagtgt gcagcagcaa
     5041 atgatgcaac aatctcgtca acttgatcca aacctgttgg tgaagcagca gactccacca
     5101 tctcagcagc agccactcca tcagccagcc atgaagtctt tccttgacaa tgtcatgccc
     5161 cacactacac ctgagctgca aaaagggcca tcaccaataa atgctttcag caacttccct
     5221 ataggcttga actcaaactt gaatgtaaat atggatatga acagtattaa agagccacag
     5281 tcaagactaa ggaagtggac gacagtggac agcatttctg tgaacacatc tttggatcaa
     5341 aactccagca aacatggtgc tatttcaagt ggtttcaggc tggaagagtc tccatttgtt
     5401 ccctatgact ttatgaacag cagtacttca ccagccagtc ctccaggttc aataggagat
     5461 ggctggccac gtgccaaatc gcctaacggc tctagcagtg ttaattggcc accagaattt
     5521 cgtcctggtg agccatggaa aggttatcca aacattgacc ctgaaactga cccttacgtc
     5581 actcctggca gtgtcataaa caatctttca attaatactg tgcgggaagt tgaccacctc
     5641 agggacagga acagtgggtc atcctcatcc ttgaacacca cgctgccttc aactagtgcc
     5701 tggtcatcca ttcgtgcctc caactacaac gttcccctca gcagtacagc acaaagcact
     5761 tcagccagaa atagtgattc caaattgaca tggtctcctg gttcagttac aaacacctct
     5821 ctggctcatg agctgtggaa ggtccctttg ccacctaaaa acatcactgc tccgtcccgc
     5881 ccacctccgg gactgactgg tcagaagcca cccttgtcta cgtgggataa ttctcccctt
     5941 cgtataggtg gaggatgggg aaattctgac gccagatata ccccaggttc cagctggggt
     6001 gagagcagct cagggagaat aacaaattgg cttgttctaa aaaaccttac acctcagatc
     6061 gatggctcaa ctctgcgcac tctgtgcatg cagcacggcc cgctgatcac attccacctg
     6121 aacctccctc acggaaatgc tctggtccgc tacagttcaa aagaagaggt agtgaaggca
     6181 caaaagtctc tgcacatgtg tgtactgggg aacactacta ttcttgctga gtttgccagt
     6241 gaagaggaga tcagtcgttt ctttgcacaa agccagtctc tgaccccttc tcccggctgg
     6301 cagtctctcg ggtccagcca gagccggctg ggctccctcg actgttccca ctcattctcc
     6361 agccggaccg atctcaatca ctggaatggt gctgggctgt cgggaactaa ctgtggagac
     6421 cttcacggca cttcactctg ggggaccccg cattattcca caagcctgtg gggtccccca
     6481 agcagcagcg acccccgagg aattagcagc ccatctccca ttaacgcttt tctttctgtt
     6541 gaccacctgg gtgggggtgg agagtccatg taatctagag ggcccgttta aacccgctga
     6601 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     6661 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     6721 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     6781 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     6841 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     6901 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     6961 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     7021 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     7081 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     7141 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     7201 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     7261 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
     7321 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     7381 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     7441 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     7501 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     7561 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     7621 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
     7681 tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
     7741 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     7801 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     7861 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
     7921 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     7981 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     8041 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     8101 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     8161 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     8221 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
     8281 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     8341 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     8401 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     8461 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     8521 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
     8581 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     8641 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     8701 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     8761 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     8821 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     8881 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     8941 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     9001 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     9061 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     9121 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     9181 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     9241 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     9301 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     9361 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     9421 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     9481 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     9541 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     9601 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     9661 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     9721 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     9781 caccgctggt agcggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     9841 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     9901 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     9961 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
    10021 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
    10081 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
    10141 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
    10201 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
    10261 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
    10321 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
    10381 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
    10441 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
    10501 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
    10561 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    10621 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    10681 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    10741 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    10801 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    10861 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    10921 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    10981 cacatttccc cgaaaagtgc cacctgacgt c
//
