LOCUS       sequence-56793-e       10840 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10840)
  AUTHORS   Nishi K, Nishi A, Nagasawa T, Ui-Tei K
  TITLE     Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated
            gene silencing in the nucleus.
  JOURNAL   RNA. 2013 Jan;19(1):17-35. doi: 10.1261/rna.034769.112. Epub 2012 
            Nov 13.
  PUBMED    23150874
REFERENCE   2  (bases 1 to 10840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.034769.112"; journalName: "RNA"; date: "2013-01"; 
            volume: "19"; issue: "1"; pages: "17-35"
COMMENT     Sequence Label: pmyc-GFP-TNRC6A-del.GW-I+II
FEATURES             Location/Qualifiers
     source          1..10840
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             910..939
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(949..951,952..954,955..1665)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(949..951,952..954,955..1665)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(994..1015)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1255..1274)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1602..1623
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    5487..5498
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     primer_bind     complement(6434..6451)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6440..6664
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6710..7138
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6797..6816)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7007..7028
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7147..7167)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7152..7481
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7332..7467
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7394..7413
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7548..8342
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7602..7621)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8212..8231
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8516..8637
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8553..8572)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8607..8626
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8686..8702)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8686..8702)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8699..8721)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8710..8726
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8734..8740,8741..8758,8759..8764))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8779..8800
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8917..8934)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9088..9673)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9168..9187)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9844..10635,10636..10704))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10467..10486
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10705..10809)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 ccaccaatgg aacaaaaact catctcagaa gaggatctgt ccggcggcat ggtgagcaag
      961 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     1021 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     1081 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     1141 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     1201 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     1261 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1321 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1381 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     1441 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1501 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     1561 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1621 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagaagct tatggatgct
     1681 gattctgcct ccagttctga atcagagaga aacatcacta tcatggcttc agggaacaca
     1741 ggtggtgaaa aagatggcct tcggaatagc actggacttg gttcccaaaa caagtttgta
     1801 gttggtagca gcagcaataa tgtgggccat ggaagtagta ctgggccatg gggtttttcc
     1861 catggagcca taataagcac atgtcaggtc tctgtggatg ctcctgaaag caaatctgaa
     1921 agtagcaaca atagaatgaa tgcttggggc actgtaagtt cttcatcaaa tggagggtta
     1981 aatccaagca ctttgaattc agctagcaac catggtgcct ggccagtatt agagaacaat
     2041 ggacttgccc taaaagggcc tgtagggagt ggtagttctg gcattaatat tcagtgcagt
     2101 actataggcc agatgcctaa caatcagagt attaactcta aagtgagtgg tggttctacc
     2161 catggtacct ggggaagcct tcaggaaact tgtgaatctg aagtaagtgg tacacagaag
     2221 gtttcattca gtggtcaacc tcaaaatatt accactgaaa tgactggacc aaataacact
     2281 actaacttta tgacctctag tttaccaaac tccggttcag tgcagaataa tgagctgcct
     2341 agtagtaaca caggggcctg gcgtgtgagc acaatgaatc atcctcagat gcaggctcca
     2401 tcaggtatga atggcacttc cctttctcac cttagcaatg gagagtcaaa aagtggaggc
     2461 tcttatggta ctacatgggg tgcctatggt tctaattact ctggagacaa atgttcaggc
     2521 cctaatggcc aagctaatgg tgacactgtg aatgcaactc taatgcagcc tggcgtaaat
     2581 ggtcctatgg gcactaactt tcaagttaac acaaacaaag gaggtggtgt gtgggaatct
     2641 ggtgcagcaa actcccagag tacatcatgg ggaagtggaa atggcgcaaa ttctggagga
     2701 agtcgaagag gatggggaac ccctgcacaa aacactggca ctaatttacc cagcgttgag
     2761 tggaacaaac tgcctagcaa tcagcattcc aatgatagtg caaatggcaa tggtaagacg
     2821 tttacaaatg gatggaaatc tactgaggaa gaggatcagg gttctgccac atctcagaca
     2881 aatgagcaaa gcagtgtgtg ggccaaaaca ggaggtacag tggagagcga tggtagtaca
     2941 gaaagcactg gacgccttga ggaaaaagga actggggaaa gtcagagtag agacagaaga
     3001 aaaattgatc agcacacatt actccaaagc attgtaaacc ccaaacctgc tctgaggtgg
     3061 ggagattcca aaggctcaaa ctgccagggg gggtgggaag atgattctgc tgctacagga
     3121 atggtcaaga gcaatcagtg ggggaattgc aaagaggaga aggctgcatg gaatgactcg
     3181 caaaagaata aacagggatg gggtgatgga caaaaatcaa gccaagggtg gtctgtttct
     3241 gccagtgata actggggaga aacttcaagg aataaccatt ggggtgaggc caataagaaa
     3301 tccagctcag gaggtagtga cagtgacagg tccgtttccg gttggaacga acttggtaaa
     3361 actagttctt tcacttgggg aaacaacata aatccaaata attcatcagg atgggatgaa
     3421 tcttctaaac ctactccttc ccagggatgg ggagaccctc caaagtctaa tcagtctcta
     3481 ggttggggag attcgtcaaa gccagtcagc tctccagact ggaacaagca acaagacatt
     3541 gttggatctt ggggaatccc accagctaca ggcaaacctc ctggtacagt cccaaatggc
     3601 aacagccgtt cagaccagca agcacaggta catcagctgc taacgcctgc aagtgccatc
     3661 tcaaacaaag aggcaagcag tggctctggc tggggtgagc cctgggggga gccttctact
     3721 ccagccacaa ctgtggataa tggtacttca gcatggggta agcccataga cagtggtccc
     3781 agctgggggg aacccattgc tgcggcatcc agcacatcca cgtggggctc cagctctgtt
     3841 ggtccacaag cattaagcaa atctgggcca aaatctatgc aagatggctg gtgtggtgat
     3901 gatatgccat tgcctggaaa tcgccccact ggctgggaag aggaagagga tgtggagatt
     3961 ggaatgtgga atagtaattc atctcaagag cttaactcat ctttaaattg gccaccatat
     4021 acaaagaaaa tgtcatcgaa gggtctgagt ggcaaaaaaa ggagaaggga aaggggaatg
     4081 atgaaaggtg gaaacaaaca agaagaagcg tggataaatc catttgttaa acagttttca
     4141 aacatcagtt tttcgagaga ctcaccagag gaaaatgtac aaagcaataa gatggacctt
     4201 tctggaggaa tgttacaaga caaacgaatg gagatagata aacatagcct aaatattggt
     4261 gattacaatc gaacggtcgg gaaaggccct ggttctcggc ctcagatttc caaagagtct
     4321 tccatggagc gcaatcctta ttttgataag gatggcattg tagcagatga atcccaaaac
     4381 atgcagttta tgtccagtca aagcatgaag cttccccctt caaatagtgc actacctaac
     4441 caggcccttg gctccatagc agggctgggt atgcaaaact tgaattctgt tagacagaat
     4501 ggcaatccca gtatgtttgg tgttggaaac acagcagcac aaccccgggg catgcagcag
     4561 cctccagcac aacctcttag ttcatctcag cctaatctcc gtgctcaagt gcctcctcca
     4621 ttactctccc ctcaggttcc agtttcattg ctgaagtatg caccaaacaa cggtggcctg
     4681 aatccactct ttggccctca acaggtagcc atgctgaacc agctatccca gctaaaccag
     4741 ctttctcaga tctcccagtt acagcgattg ttagcgcagc agcaaagggc gcagagtcag
     4801 agaagcgtgc cttctgggaa ccggccgcag caagaccagc agggtcgacc tcttagtgtg
     4861 cagcagcaaa tgatgcaaca atctcgtcaa cttgatccaa acctgttggt gaagcagcag
     4921 actccaccat ctcagcagca gccactccat cagccagcca tgaagtcttt ccttgacaat
     4981 gtcatgcccc acactacacc tgagctgcaa aaagggccat caccaataaa tgctttcagc
     5041 aacttcccta taggcttgaa ctcaaacttg aatgtaaata tggatatgaa cagtattaaa
     5101 gagccacagt caagactaag gaagtggacg acagtggaca gcatttctgt gaacacatct
     5161 ttggatcaaa actccagcaa acatggtgct atttcaagtg gtttcaggct ggaagagtct
     5221 ccatttgttc cctatgactt tatgaacagc agtacttcac cagccagtcc tccaggttca
     5281 ataggagatg gctggccacg tgccaaatcg cctaacggct ctagcagtgt taattggcca
     5341 ccagaatttc gtcctggtga gccatggaaa ggttatccaa acattgaccc tgaaactgac
     5401 ccttacgtca ctcctggcag tgtcataaac aatctttcaa ttaatactgt gcgggaagtt
     5461 gaccacctca gggacaggaa cagtgggtca tcctcatcct tgaacaccac gctgccttca
     5521 actagtgcct ggtcatccat tcgtgcctcc aactacaacg ttcccctcag cagtacagca
     5581 caaagcactt cagccagaaa tagtgattcc aaattgacat ggtctcctgg ttcagttaca
     5641 aacacctctc tggctcatga gctgtggaag gtccctttgc cacctaaaaa catcactgct
     5701 ccgtcccgcc cacctccggg actgactggt cagaagccac ccttgtctac gtgggataat
     5761 tctccccttc gtataggtgg aggatgggga aattctgacg ccagatatac cccaggttcc
     5821 agctggggtg agagcagctc agggagaata acaaattggc ttgttctaaa aaaccttaca
     5881 cctcagatcg atggctcaac tctgcgcact ctgtgcatgc agcacggccc gctgatcaca
     5941 ttccacctga acctccctca cggaaatgct ctggtccgct acagttcaaa agaagaggta
     6001 gtgaaggcac aaaagtctct gcacatgtgt gtactgggga acactactat tcttgctgag
     6061 tttgccagtg aagaggagat cagtcgtttc tttgcacaaa gccagtctct gaccccttct
     6121 cccggctggc agtctctcgg gtccagccag agccggctgg gctccctcga ctgttcccac
     6181 tcattctcca gccggaccga tctcaatcac tggaatggtg ctgggctgtc gggaactaac
     6241 tgtggagacc ttcacggcac ttcactctgg gggaccccgc attattccac aagcctgtgg
     6301 ggtcccccaa gcagcagcga cccccgagga attagcagcc catctcccat taacgctttt
     6361 ctttctgttg accacctggg tgggggtgga gagtccatgt aatctagagg gcccgtttaa
     6421 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     6481 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     6541 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     6601 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     6661 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     6721 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     6781 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     6841 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     6901 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     6961 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     7021 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     7081 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
     7141 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     7201 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     7261 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     7321 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     7381 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     7441 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
     7501 tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     7561 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     7621 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     7681 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
     7741 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     7801 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     7861 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     7921 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     7981 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     8041 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
     8101 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     8161 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     8221 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     8281 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     8341 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
     8401 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     8461 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     8521 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     8581 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     8641 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     8701 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     8761 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     8821 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     8881 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     8941 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     9001 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     9061 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     9121 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     9181 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     9241 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     9301 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     9361 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     9421 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     9481 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     9541 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     9601 caaacaaacc accgctggta gcggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     9661 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     9721 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     9781 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     9841 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     9901 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     9961 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
    10021 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
    10081 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
    10141 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
    10201 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
    10261 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
    10321 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
    10381 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
    10441 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
    10501 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
    10561 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
    10621 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    10681 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    10741 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    10801 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//
