LOCUS       sequence-56794-e       10477 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10477)
  AUTHORS   Nishi K, Nishi A, Nagasawa T, Ui-Tei K
  TITLE     Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated
            gene silencing in the nucleus.
  JOURNAL   RNA. 2013 Jan;19(1):17-35. doi: 10.1261/rna.034769.112. Epub 2012 
            Nov 13.
  PUBMED    23150874
REFERENCE   2  (bases 1 to 10477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.034769.112"; journalName: "RNA"; date: "2013-01"; 
            volume: "19"; issue: "1"; pages: "17-35"
COMMENT     Sequence Label: pmyc-GFP-TNRC6A-del.GW-I+II+III
FEATURES             Location/Qualifiers
     source          1..10477
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             910..939
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(949..951,952..954,955..1665)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(949..951,952..954,955..1665)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(994..1015)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1255..1274)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1602..1623
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    5124..5135
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     primer_bind     complement(6071..6088)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6077..6301
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6347..6775
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6434..6453)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6644..6665
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6784..6804)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6789..7118
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6969..7104
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7031..7050
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7185..7979
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7239..7258)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7849..7868
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8153..8274
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8190..8209)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8244..8263
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8323..8339)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8323..8339)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8336..8358)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8347..8363
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8371..8377,8378..8395,8396..8401))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8416..8437
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8554..8571)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8725..9310)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8805..8824)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9481..10272,10273..10341))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10104..10123
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10342..10446)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 ccaccaatgg aacaaaaact catctcagaa gaggatctgt ccggcggcat ggtgagcaag
      961 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     1021 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     1081 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     1141 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     1201 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     1261 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1321 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1381 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     1441 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1501 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     1561 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1621 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagaagct tatggatgct
     1681 gattctgcct ccagttctga atcagagaga aacatcacta tcatggcttc agggaacaca
     1741 ggtggtgaaa aagatggcct tcggaatagc actggacttg gttcccaaaa caagtttgta
     1801 gttggtagca gcagcaataa tgtgggccat ggaagtagta ctgggccatg gggtttttcc
     1861 catggagcca taataagcac atgtcaggtc tctgtggatg ctcctgaaag caaatctgaa
     1921 agtagcaaca atagaatgaa tgcttggggc actgtaagtt cttcatcaaa tggagggtta
     1981 aatccaagca ctttgaattc agctagcaac catggtgcct ggccagtatt agagaacaat
     2041 ggacttgccc taaaagggcc tgtagggagt ggtagttctg gcattaatat tcagtgcagt
     2101 actataggcc agatgcctaa caatcagagt attaactcta aagtgagtgg tggttctacc
     2161 catggtacct ggggaagcct tcaggaaact tgtgaatctg aagtaagtgg tacacagaag
     2221 gtttcattca gtggtcaacc tcaaaatatt accactgaaa tgactggacc aaataacact
     2281 actaacttta tgacctctag tttaccaaac tccggttcag tgcagaataa tgagctgcct
     2341 agtagtaaca caggggcctg gcgtgtgagc acaatgaatc atcctcagat gcaggctcca
     2401 tcaggtatga atggcacttc cctttctcac cttagcaatg gagagtcaaa aagtggaggc
     2461 tcttatggta ctacatgggg tgcctatggt tctaattact ctggagacaa atgttcaggc
     2521 cctaatggcc aagctaatgg tgacactgtg aatgcaactc taatgcagcc tggcgtaaat
     2581 ggtcctatgg gcactaactt tcaagttaac acaaacaaag gaggtggtgt gtgggaatct
     2641 ggtgcagcaa actcccagag tacatcatgg ggaagtggaa atggcgcaaa ttctggagga
     2701 agtcgaagag gatggggaac ccctgcacaa aacactggca ctaatttacc cagcgttgag
     2761 tggaacaaac tgcctagcaa tcagcattcc aatgatagtg caaatggcaa tggtaagacg
     2821 tttacaaatg gatggaaatc tactgaggaa gaggatcagg gttctgccac atctcagaca
     2881 aatgagcaaa gcagtgtgtg ggccaaaaca ggaggtacag tggagagcga tggtagtaca
     2941 gaaagcactg gacgccttga ggaaaaagga actggggaaa gtcagagtag agacagaaga
     3001 aaaattgatc agcacacatt actccaaagc attgtaaacc ccaaacctgc tctgaggtgg
     3061 ggagattcca aaggctcaaa ctgccagggg gggtgggaag atgattctgc tgctacagga
     3121 atggtcaaga gcaatcagtg ggggaattgc aaagaggaga aggctgcatg gaatgactcg
     3181 caaaagaata aacagggatg gggtgatgga caaaaatcaa gccaagggtg gtctgtttct
     3241 gccagtgata actggggaga aacttcaagg aataaccatt ggggtgaggc caataagaaa
     3301 tccagctcag gaggtagtga cagtgacagg tccgtttccg gttggaacga acttggtaaa
     3361 actagttctt tcacttgggg aaacaacata aatccaaata attcatcagg atgggatgaa
     3421 tcttctaaac ctactccttc ccagggatgg ggagaccctc caaagtctaa tcagtctcta
     3481 ggttggggag attcgtcaaa gccagtcagc tctccagact ggaacaagca acaagacatt
     3541 gttggatctt ggggaatccc accagctaca ggcaaacctc ctggtacagt cccaaatggc
     3601 aacagccgtt cagaccagca agcacaggta catcagctgc taacgcctgc aagtgccatc
     3661 tcaaacaaag aggcaagcag tctgagtggc aaaaaaagga gaagggaaag gggaatgatg
     3721 aaaggtggaa acaaacaaga agaagcgtgg ataaatccat ttgttaaaca gttttcaaac
     3781 atcagttttt cgagagactc accagaggaa aatgtacaaa gcaataagat ggacctttct
     3841 ggaggaatgt tacaagacaa acgaatggag atagataaac atagcctaaa tattggtgat
     3901 tacaatcgaa cggtcgggaa aggccctggt tctcggcctc agatttccaa agagtcttcc
     3961 atggagcgca atccttattt tgataaggat ggcattgtag cagatgaatc ccaaaacatg
     4021 cagtttatgt ccagtcaaag catgaagctt cccccttcaa atagtgcact acctaaccag
     4081 gcccttggct ccatagcagg gctgggtatg caaaacttga attctgttag acagaatggc
     4141 aatcccagta tgtttggtgt tggaaacaca gcagcacaac cccggggcat gcagcagcct
     4201 ccagcacaac ctcttagttc atctcagcct aatctccgtg ctcaagtgcc tcctccatta
     4261 ctctcccctc aggttccagt ttcattgctg aagtatgcac caaacaacgg tggcctgaat
     4321 ccactctttg gccctcaaca ggtagccatg ctgaaccagc tatcccagct aaaccagctt
     4381 tctcagatct cccagttaca gcgattgtta gcgcagcagc aaagggcgca gagtcagaga
     4441 agcgtgcctt ctgggaaccg gccgcagcaa gaccagcagg gtcgacctct tagtgtgcag
     4501 cagcaaatga tgcaacaatc tcgtcaactt gatccaaacc tgttggtgaa gcagcagact
     4561 ccaccatctc agcagcagcc actccatcag ccagccatga agtctttcct tgacaatgtc
     4621 atgccccaca ctacacctga gctgcaaaaa gggccatcac caataaatgc tttcagcaac
     4681 ttccctatag gcttgaactc aaacttgaat gtaaatatgg atatgaacag tattaaagag
     4741 ccacagtcaa gactaaggaa gtggacgaca gtggacagca tttctgtgaa cacatctttg
     4801 gatcaaaact ccagcaaaca tggtgctatt tcaagtggtt tcaggctgga agagtctcca
     4861 tttgttccct atgactttat gaacagcagt acttcaccag ccagtcctcc aggttcaata
     4921 ggagatggct ggccacgtgc caaatcgcct aacggctcta gcagtgttaa ttggccacca
     4981 gaatttcgtc ctggtgagcc atggaaaggt tatccaaaca ttgaccctga aactgaccct
     5041 tacgtcactc ctggcagtgt cataaacaat ctttcaatta atactgtgcg ggaagttgac
     5101 cacctcaggg acaggaacag tgggtcatcc tcatccttga acaccacgct gccttcaact
     5161 agtgcctggt catccattcg tgcctccaac tacaacgttc ccctcagcag tacagcacaa
     5221 agcacttcag ccagaaatag tgattccaaa ttgacatggt ctcctggttc agttacaaac
     5281 acctctctgg ctcatgagct gtggaaggtc cctttgccac ctaaaaacat cactgctccg
     5341 tcccgcccac ctccgggact gactggtcag aagccaccct tgtctacgtg ggataattct
     5401 ccccttcgta taggtggagg atggggaaat tctgacgcca gatatacccc aggttccagc
     5461 tggggtgaga gcagctcagg gagaataaca aattggcttg ttctaaaaaa ccttacacct
     5521 cagatcgatg gctcaactct gcgcactctg tgcatgcagc acggcccgct gatcacattc
     5581 cacctgaacc tccctcacgg aaatgctctg gtccgctaca gttcaaaaga agaggtagtg
     5641 aaggcacaaa agtctctgca catgtgtgta ctggggaaca ctactattct tgctgagttt
     5701 gccagtgaag aggagatcag tcgtttcttt gcacaaagcc agtctctgac cccttctccc
     5761 ggctggcagt ctctcgggtc cagccagagc cggctgggct ccctcgactg ttcccactca
     5821 ttctccagcc ggaccgatct caatcactgg aatggtgctg ggctgtcggg aactaactgt
     5881 ggagaccttc acggcacttc actctggggg accccgcatt attccacaag cctgtggggt
     5941 cccccaagca gcagcgaccc ccgaggaatt agcagcccat ctcccattaa cgcttttctt
     6001 tctgttgacc acctgggtgg gggtggagag tccatgtaat ctagagggcc cgtttaaacc
     6061 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     6121 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     6181 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     6241 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     6301 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
     6361 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     6421 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     6481 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     6541 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     6601 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     6661 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     6721 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc
     6781 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     6841 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     6901 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     6961 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     7021 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     7081 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     7141 ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
     7201 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     7261 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     7321 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc
     7381 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     7441 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
     7501 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     7561 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     7621 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     7681 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg
     7741 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     7801 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     7861 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     7921 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
     7981 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
     8041 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     8101 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
     8161 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     8221 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     8281 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
     8341 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     8401 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     8461 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     8521 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     8581 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     8641 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     8701 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     8761 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     8821 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     8881 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     8941 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     9001 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     9061 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     9121 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     9181 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     9241 acaaaccacc gctggtagcg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     9301 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     9361 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     9421 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     9481 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     9541 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     9601 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     9661 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     9721 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     9781 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     9841 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     9901 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     9961 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
    10021 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    10081 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    10141 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    10201 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    10261 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    10321 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    10381 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    10441 tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
