LOCUS       sequence-56801-e       10876 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10876)
  AUTHORS   Nishi K, Nishi A, Nagasawa T, Ui-Tei K
  TITLE     Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated
            gene silencing in the nucleus.
  JOURNAL   RNA. 2013 Jan;19(1):17-35. doi: 10.1261/rna.034769.112. Epub 2012 
            Nov 13.
  PUBMED    23150874
REFERENCE   2  (bases 1 to 10876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.034769.112"; journalName: "RNA"; date: "2013-01"; 
            volume: "19"; issue: "1"; pages: "17-35"
COMMENT     Sequence Label: pmyc-GFP-TNRC6A-del.GW-III-NES-mut
FEATURES             Location/Qualifiers
     source          1..10876
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             910..939
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(949..951,952..954,955..1665)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(949..951,952..954,955..1665)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(994..1015)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1255..1274)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1602..1623
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    5523..5534
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     primer_bind     complement(6470..6487)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6476..6700
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6746..7174
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6833..6852)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7043..7064
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7183..7203)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7188..7517
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7368..7503
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7430..7449
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7584..8378
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7638..7657)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8248..8267
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8552..8673
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8589..8608)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8643..8662
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8722..8738)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8722..8738)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8735..8757)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8746..8762
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8770..8776,8777..8794,8795..8800))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8815..8836
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8953..8970)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9124..9709)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9204..9223)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9880..10671,10672..10740))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10503..10522
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10741..10845)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 ccaccaatgg aacaaaaact catctcagaa gaggatctgt ccggcggcat ggtgagcaag
      961 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     1021 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     1081 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     1141 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     1201 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     1261 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1321 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1381 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     1441 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1501 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     1561 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1621 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagaagct tatggatgct
     1681 gattctgcct ccagttctga atcagagaga aacatcacta tcatggcttc agggaacaca
     1741 ggtggtgaaa aagatggcct tcggaatagc actggacttg gttcccaaaa caagtttgta
     1801 gttggtagca gcagcaataa tgtgggccat ggaagtagta ctgggccatg gggtttttcc
     1861 catggagcca taataagcac atgtcaggtc tctgtggatg ctcctgaaag caaatctgaa
     1921 agtagcaaca atagaatgaa tgcttggggc actgtaagtt cttcatcaaa tggagggtta
     1981 aatccaagca ctttgaattc agctagcaac catggtgcct ggccagtatt agagaacaat
     2041 ggacttgccc taaaagggcc tgtagggagt ggtagttctg gcattaatat tcagtgcagt
     2101 actataggcc agatgcctaa caatcagagt attaactcta aagtgagtgg tggttctacc
     2161 catggtacct ggggaagcct tcaggaaact tgtgaatctg aagtaagtgg tacacagaag
     2221 gtttcattca gtggtcaacc tcaaaatatt accactgaaa tgactggacc aaataacact
     2281 actaacttta tgacctctag tttaccaaac tccggttcag tgcagaataa tgagctgcct
     2341 agtagtaaca caggggcctg gcgtgtgagc acaatgaatc atcctcagat gcaggctcca
     2401 tcaggtatga atggcacttc cctttctcac cttagcaatg gagagtcaaa aagtggaggc
     2461 tcttatggta ctacatgggg tgcctatggt tctaattact ctggagacaa atgttcaggc
     2521 cctaatggcc aagctaatgg tgacactgtg aatgcaactc taatgcagcc tggcgtaaat
     2581 ggtcctatgg gcactaactt tcaagttaac acaaacaaag gaggtggtgt gtgggaatct
     2641 ggtgcagcaa actcccagag tacatcatgg ggaagtggaa atggcgcaaa ttctggagga
     2701 agtcgaagag gatggggaac ccctgcacaa aacactggca ctaatttacc cagcgttgag
     2761 tggaacaaac tgcctagcaa tcagcattcc aatgatagtg caaatggcaa tggtaagacg
     2821 tttacaaatg gatggaaatc tactgaggaa gaggatcagg gttctgccac atctcagaca
     2881 aatgagcaaa gcagtgtgtg ggccaaaaca ggaggtacag tggagagcga tggtagtaca
     2941 gaaagcactg gacgccttga ggaaaaagga actggggaaa gtcagagtag agacagaaga
     3001 aaaattgatc agcacacatt actccaaagc attgtaaaca gaactgactt agatccacgt
     3061 gtcctgtcca actctggttg gggacagact cctattaagc agaatactgc ctgggataca
     3121 gaaacatcac ctagagggga acgaaagact gacaatggga cagaggcctg gggaagctct
     3181 gcaacacaga cttttaactc aggggcatgt atagataaga ctagccctaa tggtaatgat
     3241 acctcatctg tatcagggtg gggcgatccc aaacctgctc tgaggtgggg agattccaaa
     3301 ggctcaaact gccagggggg gtgggaagat gattctgctg ctacaggaat ggtcaagagc
     3361 aatcagtggg ggaattgcaa agaggagaag gctgcatgga atgactcgca aaagaataaa
     3421 cagggatggg gtgatggaca aaaatcaagc caagggtggt ctgtttctgc cagtgataac
     3481 tggggagaaa cttcaaggaa taaccattgg ggtgaggcca ataagaaatc cagctcagga
     3541 ggtagtgaca gtgacaggtc cgtttccggt tggaacgaac ttggtaaaac tagttctttc
     3601 acttggggaa acaacataaa tccaaataat tcatcaggat gggatgaatc ttctaaacct
     3661 actccttccc agggatgggg agaccctcca aagtctaatc agtctctagg ttggggagat
     3721 tcgtcaaagc cagtcagctc tccagactgg aacaagcaac aagacattgt tggatcttgg
     3781 ggaatcccac cagctacagg caaacctcct ggtacaggct ggctgggggg acctatacca
     3841 gccccagcaa aagaagaaga acccacaggc tgggaggaac catccccaga atctatacgt
     3901 cgcaaaatgg agattgatga tggaacttca gcttggggag atccaagcaa atacaactac
     3961 aaaaatgtga acatgtggaa caaaaacgtc ccaaatggca acagccgttc agaccagcaa
     4021 gcacaggtac atcagctgct aacgcctgca agtgccatct caaacaaaga ggcaagcagt
     4081 ctgagtggca aaaaaaggag aagggaaagg ggaatgatga aaggtggaaa caaacaagaa
     4141 gaagcgtgga taaatccagc cgttaaacag gcttcaaacg ccagtgcttc gagagactca
     4201 ccagaggaaa atgtacaaag caataagatg gacctttctg gaggaatgtt acaagacaaa
     4261 cgaatggaga tagataaaca tagcctaaat attggtgatt acaatcgaac ggtcgggaaa
     4321 ggccctggtt ctcggcctca gatttccaaa gagtcttcca tggagcgcaa tccttatttt
     4381 gataaggatg gcattgtagc agatgaatcc caaaacatgc agtttatgtc cagtcaaagc
     4441 atgaagcttc ccccttcaaa tagtgcacta cctaaccagg cccttggctc catagcaggg
     4501 ctgggtatgc aaaacttgaa ttctgttaga cagaatggca atcccagtat gtttggtgtt
     4561 ggaaacacag cagcacaacc ccggggcatg cagcagcctc cagcacaacc tcttagttca
     4621 tctcagccta atctccgtgc tcaagtgcct cctccattac tctcccctca ggttccagtt
     4681 tcattgctga agtatgcacc aaacaacggt ggcctgaatc cactctttgg ccctcaacag
     4741 gtagccatgc tgaaccagct atcccagcta aaccagcttt ctcagatctc ccagttacag
     4801 cgattgttag cgcagcagca aagggcgcag agtcagagaa gcgtgccttc tgggaaccgg
     4861 ccgcagcaag accagcaggg tcgacctctt agtgtgcagc agcaaatgat gcaacaatct
     4921 cgtcaacttg atccaaacct gttggtgaag cagcagactc caccatctca gcagcagcca
     4981 ctccatcagc cagccatgaa gtctttcctt gacaatgtca tgccccacac tacacctgag
     5041 ctgcaaaaag ggccatcacc aataaatgct ttcagcaact tccctatagg cttgaactca
     5101 aacttgaatg taaatatgga tatgaacagt attaaagagc cacagtcaag actaaggaag
     5161 tggacgacag tggacagcat ttctgtgaac acatctttgg atcaaaactc cagcaaacat
     5221 ggtgctattt caagtggttt caggctggaa gagtctccat ttgttcccta tgactttatg
     5281 aacagcagta cttcaccagc cagtcctcca ggttcaatag gagatggctg gccacgtgcc
     5341 aaatcgccta acggctctag cagtgttaat tggccaccag aatttcgtcc tggtgagcca
     5401 tggaaaggtt atccaaacat tgaccctgaa actgaccctt acgtcactcc tggcagtgtc
     5461 ataaacaatc tttcaattaa tactgtgcgg gaagttgacc acctcaggga caggaacagt
     5521 gggtcatcct catccttgaa caccacgctg ccttcaacta gtgcctggtc atccattcgt
     5581 gcctccaact acaacgttcc cctcagcagt acagcacaaa gcacttcagc cagaaatagt
     5641 gattccaaat tgacatggtc tcctggttca gttacaaaca cctctctggc tcatgagctg
     5701 tggaaggtcc ctttgccacc taaaaacatc actgctccgt cccgcccacc tccgggactg
     5761 actggtcaga agccaccctt gtctacgtgg gataattctc cccttcgtat aggtggagga
     5821 tggggaaatt ctgacgccag atatacccca ggttccagct ggggtgagag cagctcaggg
     5881 agaataacaa attggcttgt tctaaaaaac cttacacctc agatcgatgg ctcaactctg
     5941 cgcactctgt gcatgcagca cggcccgctg atcacattcc acctgaacct ccctcacgga
     6001 aatgctctgg tccgctacag ttcaaaagaa gaggtagtga aggcacaaaa gtctctgcac
     6061 atgtgtgtac tggggaacac tactattctt gctgagtttg ccagtgaaga ggagatcagt
     6121 cgtttctttg cacaaagcca gtctctgacc ccttctcccg gctggcagtc tctcgggtcc
     6181 agccagagcc ggctgggctc cctcgactgt tcccactcat tctccagccg gaccgatctc
     6241 aatcactgga atggtgctgg gctgtcggga actaactgtg gagaccttca cggcacttca
     6301 ctctggggga ccccgcatta ttccacaagc ctgtggggtc ccccaagcag cagcgacccc
     6361 cgaggaatta gcagcccatc tcccattaac gcttttcttt ctgttgacca cctgggtggg
     6421 ggtggagagt ccatgtaatc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg
     6481 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     6541 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     6601 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     6661 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     6721 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     6781 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     6841 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     6901 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     6961 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     7021 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     7081 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     7141 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
     7201 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     7261 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     7321 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     7381 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     7441 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     7501 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag
     7561 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
     7621 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     7681 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     7741 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga
     7801 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     7861 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     7921 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     7981 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     8041 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     8101 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
     8161 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     8221 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     8281 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     8341 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
     8401 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
     8461 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     8521 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     8581 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     8641 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     8701 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     8761 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     8821 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     8881 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     8941 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     9001 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     9061 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     9121 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     9181 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     9241 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     9301 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     9361 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     9421 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     9481 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     9541 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     9601 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     9661 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     9721 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     9781 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     9841 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     9901 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     9961 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
    10021 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    10081 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    10141 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    10201 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    10261 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    10321 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    10381 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    10441 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    10501 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    10561 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    10621 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    10681 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    10741 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    10801 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    10861 agtgccacct gacgtc
//
