LOCUS       sequence-56802-e       10648 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10648)
  AUTHORS   Nishi K, Nishi A, Nagasawa T, Ui-Tei K
  TITLE     Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated
            gene silencing in the nucleus.
  JOURNAL   RNA. 2013 Jan;19(1):17-35. doi: 10.1261/rna.034769.112. Epub 2012 
            Nov 13.
  PUBMED    23150874
REFERENCE   2  (bases 1 to 10648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.034769.112"; journalName: "RNA"; date: "2013-01"; 
            volume: "19"; issue: "1"; pages: "17-35"
COMMENT     Sequence Label: pmyc-GFP-TNRC6A-del.GW-I+III-NES-mut
FEATURES             Location/Qualifiers
     source          1..10648
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             910..939
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(949..951,952..954,955..1665)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(949..951,952..954,955..1665)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(994..1015)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1255..1274)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1602..1623
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    5295..5306
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     primer_bind     complement(6242..6259)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6248..6472
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6518..6946
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6605..6624)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6815..6836
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6955..6975)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6960..7289
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7140..7275
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7202..7221
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7356..8150
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7410..7429)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8020..8039
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8324..8445
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8361..8380)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8415..8434
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8494..8510)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8494..8510)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8507..8529)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8518..8534
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8542..8548,8549..8566,8567..8572))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8587..8608
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8725..8742)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8896..9481)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8976..8995)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9652..10443,10444..10512))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10275..10294
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10513..10617)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 ccaccaatgg aacaaaaact catctcagaa gaggatctgt ccggcggcat ggtgagcaag
      961 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     1021 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     1081 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     1141 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     1201 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     1261 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1321 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1381 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     1441 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1501 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     1561 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1621 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagaagct tatggatgct
     1681 gattctgcct ccagttctga atcagagaga aacatcacta tcatggcttc agggaacaca
     1741 ggtggtgaaa aagatggcct tcggaatagc actggacttg gttcccaaaa caagtttgta
     1801 gttggtagca gcagcaataa tgtgggccat ggaagtagta ctgggccatg gggtttttcc
     1861 catggagcca taataagcac atgtcaggtc tctgtggatg ctcctgaaag caaatctgaa
     1921 agtagcaaca atagaatgaa tgcttggggc actgtaagtt cttcatcaaa tggagggtta
     1981 aatccaagca ctttgaattc agctagcaac catggtgcct ggccagtatt agagaacaat
     2041 ggacttgccc taaaagggcc tgtagggagt ggtagttctg gcattaatat tcagtgcagt
     2101 actataggcc agatgcctaa caatcagagt attaactcta aagtgagtgg tggttctacc
     2161 catggtacct ggggaagcct tcaggaaact tgtgaatctg aagtaagtgg tacacagaag
     2221 gtttcattca gtggtcaacc tcaaaatatt accactgaaa tgactggacc aaataacact
     2281 actaacttta tgacctctag tttaccaaac tccggttcag tgcagaataa tgagctgcct
     2341 agtagtaaca caggggcctg gcgtgtgagc acaatgaatc atcctcagat gcaggctcca
     2401 tcaggtatga atggcacttc cctttctcac cttagcaatg gagagtcaaa aagtggaggc
     2461 tcttatggta ctacatgggg tgcctatggt tctaattact ctggagacaa atgttcaggc
     2521 cctaatggcc aagctaatgg tgacactgtg aatgcaactc taatgcagcc tggcgtaaat
     2581 ggtcctatgg gcactaactt tcaagttaac acaaacaaag gaggtggtgt gtgggaatct
     2641 ggtgcagcaa actcccagag tacatcatgg ggaagtggaa atggcgcaaa ttctggagga
     2701 agtcgaagag gatggggaac ccctgcacaa aacactggca ctaatttacc cagcgttgag
     2761 tggaacaaac tgcctagcaa tcagcattcc aatgatagtg caaatggcaa tggtaagacg
     2821 tttacaaatg gatggaaatc tactgaggaa gaggatcagg gttctgccac atctcagaca
     2881 aatgagcaaa gcagtgtgtg ggccaaaaca ggaggtacag tggagagcga tggtagtaca
     2941 gaaagcactg gacgccttga ggaaaaagga actggggaaa gtcagagtag agacagaaga
     3001 aaaattgatc agcacacatt actccaaagc attgtaaacc ccaaacctgc tctgaggtgg
     3061 ggagattcca aaggctcaaa ctgccagggg gggtgggaag atgattctgc tgctacagga
     3121 atggtcaaga gcaatcagtg ggggaattgc aaagaggaga aggctgcatg gaatgactcg
     3181 caaaagaata aacagggatg gggtgatgga caaaaatcaa gccaagggtg gtctgtttct
     3241 gccagtgata actggggaga aacttcaagg aataaccatt ggggtgaggc caataagaaa
     3301 tccagctcag gaggtagtga cagtgacagg tccgtttccg gttggaacga acttggtaaa
     3361 actagttctt tcacttgggg aaacaacata aatccaaata attcatcagg atgggatgaa
     3421 tcttctaaac ctactccttc ccagggatgg ggagaccctc caaagtctaa tcagtctcta
     3481 ggttggggag attcgtcaaa gccagtcagc tctccagact ggaacaagca acaagacatt
     3541 gttggatctt ggggaatccc accagctaca ggcaaacctc ctggtacagg ctggctgggg
     3601 ggacctatac cagccccagc aaaagaagaa gaacccacag gctgggagga accatcccca
     3661 gaatctatac gtcgcaaaat ggagattgat gatggaactt cagcttgggg agatccaagc
     3721 aaatacaact acaaaaatgt gaacatgtgg aacaaaaacg tcccaaatgg caacagccgt
     3781 tcagaccagc aagcacaggt acatcagctg ctaacgcctg caagtgccat ctcaaacaaa
     3841 gaggcaagca gtctgagtgg caaaaaaagg agaagggaaa ggggaatgat gaaaggtgga
     3901 aacaaacaag aagaagcgtg gataaatcca gccgttaaac aggcttcaaa cgccagtgct
     3961 tcgagagact caccagagga aaatgtacaa agcaataaga tggacctttc tggaggaatg
     4021 ttacaagaca aacgaatgga gatagataaa catagcctaa atattggtga ttacaatcga
     4081 acggtcggga aaggccctgg ttctcggcct cagatttcca aagagtcttc catggagcgc
     4141 aatccttatt ttgataagga tggcattgta gcagatgaat cccaaaacat gcagtttatg
     4201 tccagtcaaa gcatgaagct tcccccttca aatagtgcac tacctaacca ggcccttggc
     4261 tccatagcag ggctgggtat gcaaaacttg aattctgtta gacagaatgg caatcccagt
     4321 atgtttggtg ttggaaacac agcagcacaa ccccggggca tgcagcagcc tccagcacaa
     4381 cctcttagtt catctcagcc taatctccgt gctcaagtgc ctcctccatt actctcccct
     4441 caggttccag tttcattgct gaagtatgca ccaaacaacg gtggcctgaa tccactcttt
     4501 ggccctcaac aggtagccat gctgaaccag ctatcccagc taaaccagct ttctcagatc
     4561 tcccagttac agcgattgtt agcgcagcag caaagggcgc agagtcagag aagcgtgcct
     4621 tctgggaacc ggccgcagca agaccagcag ggtcgacctc ttagtgtgca gcagcaaatg
     4681 atgcaacaat ctcgtcaact tgatccaaac ctgttggtga agcagcagac tccaccatct
     4741 cagcagcagc cactccatca gccagccatg aagtctttcc ttgacaatgt catgccccac
     4801 actacacctg agctgcaaaa agggccatca ccaataaatg ctttcagcaa cttccctata
     4861 ggcttgaact caaacttgaa tgtaaatatg gatatgaaca gtattaaaga gccacagtca
     4921 agactaagga agtggacgac agtggacagc atttctgtga acacatcttt ggatcaaaac
     4981 tccagcaaac atggtgctat ttcaagtggt ttcaggctgg aagagtctcc atttgttccc
     5041 tatgacttta tgaacagcag tacttcacca gccagtcctc caggttcaat aggagatggc
     5101 tggccacgtg ccaaatcgcc taacggctct agcagtgtta attggccacc agaatttcgt
     5161 cctggtgagc catggaaagg ttatccaaac attgaccctg aaactgaccc ttacgtcact
     5221 cctggcagtg tcataaacaa tctttcaatt aatactgtgc gggaagttga ccacctcagg
     5281 gacaggaaca gtgggtcatc ctcatccttg aacaccacgc tgccttcaac tagtgcctgg
     5341 tcatccattc gtgcctccaa ctacaacgtt cccctcagca gtacagcaca aagcacttca
     5401 gccagaaata gtgattccaa attgacatgg tctcctggtt cagttacaaa cacctctctg
     5461 gctcatgagc tgtggaaggt ccctttgcca cctaaaaaca tcactgctcc gtcccgccca
     5521 cctccgggac tgactggtca gaagccaccc ttgtctacgt gggataattc tccccttcgt
     5581 ataggtggag gatggggaaa ttctgacgcc agatataccc caggttccag ctggggtgag
     5641 agcagctcag ggagaataac aaattggctt gttctaaaaa accttacacc tcagatcgat
     5701 ggctcaactc tgcgcactct gtgcatgcag cacggcccgc tgatcacatt ccacctgaac
     5761 ctccctcacg gaaatgctct ggtccgctac agttcaaaag aagaggtagt gaaggcacaa
     5821 aagtctctgc acatgtgtgt actggggaac actactattc ttgctgagtt tgccagtgaa
     5881 gaggagatca gtcgtttctt tgcacaaagc cagtctctga ccccttctcc cggctggcag
     5941 tctctcgggt ccagccagag ccggctgggc tccctcgact gttcccactc attctccagc
     6001 cggaccgatc tcaatcactg gaatggtgct gggctgtcgg gaactaactg tggagacctt
     6061 cacggcactt cactctgggg gaccccgcat tattccacaa gcctgtgggg tcccccaagc
     6121 agcagcgacc cccgaggaat tagcagccca tctcccatta acgcttttct ttctgttgac
     6181 cacctgggtg ggggtggaga gtccatgtaa tctagagggc ccgtttaaac ccgctgatca
     6241 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     6301 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     6361 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     6421 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     6481 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     6541 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     6601 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     6661 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     6721 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     6781 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     6841 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     6901 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     6961 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     7021 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     7081 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     7141 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     7201 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     7261 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     7321 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     7381 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     7441 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     7501 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     7561 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     7621 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     7681 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     7741 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     7801 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     7861 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     7921 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     7981 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     8041 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     8101 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     8161 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     8221 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     8281 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
     8341 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     8401 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
     8461 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     8521 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     8581 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     8641 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     8701 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     8761 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     8821 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     8881 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     8941 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     9001 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     9061 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     9121 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     9181 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     9241 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     9301 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     9361 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     9421 cgctggtagc ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     9481 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     9541 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     9601 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     9661 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     9721 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     9781 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     9841 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     9901 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     9961 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
    10021 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
    10081 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
    10141 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    10201 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    10261 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    10321 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    10381 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    10441 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    10501 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    10561 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    10621 atttccccga aaagtgccac ctgacgtc
//
