LOCUS       sequence-56803-e       10705 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10705)
  AUTHORS   Nishi K, Nishi A, Nagasawa T, Ui-Tei K
  TITLE     Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated
            gene silencing in the nucleus.
  JOURNAL   RNA. 2013 Jan;19(1):17-35. doi: 10.1261/rna.034769.112. Epub 2012 
            Nov 13.
  PUBMED    23150874
REFERENCE   2  (bases 1 to 10705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.034769.112"; journalName: "RNA"; date: "2013-01"; 
            volume: "19"; issue: "1"; pages: "17-35"
COMMENT     Sequence Label: pmyc-GFP-TNRC6A-del.GW-II+III-NES-mut
FEATURES             Location/Qualifiers
     source          1..10705
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             910..939
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(949..951,952..954,955..1665)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(949..951,952..954,955..1665)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(994..1015)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1255..1274)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1602..1623
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    5352..5363
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     primer_bind     complement(6299..6316)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6305..6529
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6575..7003
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6662..6681)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6872..6893
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7012..7032)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7017..7346
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7197..7332
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7259..7278
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7413..8207
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7467..7486)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8077..8096
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8381..8502
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8418..8437)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8472..8491
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8551..8567)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8551..8567)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8564..8586)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8575..8591
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8599..8605,8606..8623,8624..8629))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8644..8665
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8782..8799)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8953..9538)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9033..9052)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9709..10500,10501..10569))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10332..10351
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10570..10674)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 ccaccaatgg aacaaaaact catctcagaa gaggatctgt ccggcggcat ggtgagcaag
      961 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     1021 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     1081 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     1141 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     1201 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     1261 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1321 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1381 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     1441 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1501 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     1561 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1621 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagaagct tatggatgct
     1681 gattctgcct ccagttctga atcagagaga aacatcacta tcatggcttc agggaacaca
     1741 ggtggtgaaa aagatggcct tcggaatagc actggacttg gttcccaaaa caagtttgta
     1801 gttggtagca gcagcaataa tgtgggccat ggaagtagta ctgggccatg gggtttttcc
     1861 catggagcca taataagcac atgtcaggtc tctgtggatg ctcctgaaag caaatctgaa
     1921 agtagcaaca atagaatgaa tgcttggggc actgtaagtt cttcatcaaa tggagggtta
     1981 aatccaagca ctttgaattc agctagcaac catggtgcct ggccagtatt agagaacaat
     2041 ggacttgccc taaaagggcc tgtagggagt ggtagttctg gcattaatat tcagtgcagt
     2101 actataggcc agatgcctaa caatcagagt attaactcta aagtgagtgg tggttctacc
     2161 catggtacct ggggaagcct tcaggaaact tgtgaatctg aagtaagtgg tacacagaag
     2221 gtttcattca gtggtcaacc tcaaaatatt accactgaaa tgactggacc aaataacact
     2281 actaacttta tgacctctag tttaccaaac tccggttcag tgcagaataa tgagctgcct
     2341 agtagtaaca caggggcctg gcgtgtgagc acaatgaatc atcctcagat gcaggctcca
     2401 tcaggtatga atggcacttc cctttctcac cttagcaatg gagagtcaaa aagtggaggc
     2461 tcttatggta ctacatgggg tgcctatggt tctaattact ctggagacaa atgttcaggc
     2521 cctaatggcc aagctaatgg tgacactgtg aatgcaactc taatgcagcc tggcgtaaat
     2581 ggtcctatgg gcactaactt tcaagttaac acaaacaaag gaggtggtgt gtgggaatct
     2641 ggtgcagcaa actcccagag tacatcatgg ggaagtggaa atggcgcaaa ttctggagga
     2701 agtcgaagag gatggggaac ccctgcacaa aacactggca ctaatttacc cagcgttgag
     2761 tggaacaaac tgcctagcaa tcagcattcc aatgatagtg caaatggcaa tggtaagacg
     2821 tttacaaatg gatggaaatc tactgaggaa gaggatcagg gttctgccac atctcagaca
     2881 aatgagcaaa gcagtgtgtg ggccaaaaca ggaggtacag tggagagcga tggtagtaca
     2941 gaaagcactg gacgccttga ggaaaaagga actggggaaa gtcagagtag agacagaaga
     3001 aaaattgatc agcacacatt actccaaagc attgtaaaca gaactgactt agatccacgt
     3061 gtcctgtcca actctggttg gggacagact cctattaagc agaatactgc ctgggataca
     3121 gaaacatcac ctagagggga acgaaagact gacaatggga cagaggcctg gggaagctct
     3181 gcaacacaga cttttaactc aggggcatgt atagataaga ctagccctaa tggtaatgat
     3241 acctcatctg tatcagggtg gggcgatccc aaacctgctc tgaggtgggg agattccaaa
     3301 ggctcaaact gccagggggg gtgggaagat gattctgctg ctacaggaat ggtcaagagc
     3361 aatcagtggg ggaattgcaa agaggagaag gctgcatgga atgactcgca aaagaataaa
     3421 cagggatggg gtgatggaca aaaatcaagc caagggtggt ctgtttctgc cagtgataac
     3481 tggggagaaa cttcaaggaa taaccattgg ggtgaggcca ataagaaatc cagctcagga
     3541 ggtagtgaca gtgacaggtc cgtttccggt tggaacgaac ttggtaaaac tagttctttc
     3601 acttggggaa acaacataaa tccaaataat tcatcaggat gggatgaatc ttctaaacct
     3661 actccttccc agggatgggg agaccctcca aagtctaatc agtctctagg ttggggagat
     3721 tcgtcaaagc cagtcagctc tccagactgg aacaagcaac aagacattgt tggatcttgg
     3781 ggaatcccac cagctacagg caaacctcct ggtacagtcc caaatggcaa cagccgttca
     3841 gaccagcaag cacaggtaca tcagctgcta acgcctgcaa gtgccatctc aaacaaagag
     3901 gcaagcagtc tgagtggcaa aaaaaggaga agggaaaggg gaatgatgaa aggtggaaac
     3961 aaacaagaag aagcgtggat aaatccagcc gttaaacagg cttcaaacgc cagtgcttcg
     4021 agagactcac cagaggaaaa tgtacaaagc aataagatgg acctttctgg aggaatgtta
     4081 caagacaaac gaatggagat agataaacat agcctaaata ttggtgatta caatcgaacg
     4141 gtcgggaaag gccctggttc tcggcctcag atttccaaag agtcttccat ggagcgcaat
     4201 ccttattttg ataaggatgg cattgtagca gatgaatccc aaaacatgca gtttatgtcc
     4261 agtcaaagca tgaagcttcc cccttcaaat agtgcactac ctaaccaggc ccttggctcc
     4321 atagcagggc tgggtatgca aaacttgaat tctgttagac agaatggcaa tcccagtatg
     4381 tttggtgttg gaaacacagc agcacaaccc cggggcatgc agcagcctcc agcacaacct
     4441 cttagttcat ctcagcctaa tctccgtgct caagtgcctc ctccattact ctcccctcag
     4501 gttccagttt cattgctgaa gtatgcacca aacaacggtg gcctgaatcc actctttggc
     4561 cctcaacagg tagccatgct gaaccagcta tcccagctaa accagctttc tcagatctcc
     4621 cagttacagc gattgttagc gcagcagcaa agggcgcaga gtcagagaag cgtgccttct
     4681 gggaaccggc cgcagcaaga ccagcagggt cgacctctta gtgtgcagca gcaaatgatg
     4741 caacaatctc gtcaacttga tccaaacctg ttggtgaagc agcagactcc accatctcag
     4801 cagcagccac tccatcagcc agccatgaag tctttccttg acaatgtcat gccccacact
     4861 acacctgagc tgcaaaaagg gccatcacca ataaatgctt tcagcaactt ccctataggc
     4921 ttgaactcaa acttgaatgt aaatatggat atgaacagta ttaaagagcc acagtcaaga
     4981 ctaaggaagt ggacgacagt ggacagcatt tctgtgaaca catctttgga tcaaaactcc
     5041 agcaaacatg gtgctatttc aagtggtttc aggctggaag agtctccatt tgttccctat
     5101 gactttatga acagcagtac ttcaccagcc agtcctccag gttcaatagg agatggctgg
     5161 ccacgtgcca aatcgcctaa cggctctagc agtgttaatt ggccaccaga atttcgtcct
     5221 ggtgagccat ggaaaggtta tccaaacatt gaccctgaaa ctgaccctta cgtcactcct
     5281 ggcagtgtca taaacaatct ttcaattaat actgtgcggg aagttgacca cctcagggac
     5341 aggaacagtg ggtcatcctc atccttgaac accacgctgc cttcaactag tgcctggtca
     5401 tccattcgtg cctccaacta caacgttccc ctcagcagta cagcacaaag cacttcagcc
     5461 agaaatagtg attccaaatt gacatggtct cctggttcag ttacaaacac ctctctggct
     5521 catgagctgt ggaaggtccc tttgccacct aaaaacatca ctgctccgtc ccgcccacct
     5581 ccgggactga ctggtcagaa gccacccttg tctacgtggg ataattctcc ccttcgtata
     5641 ggtggaggat ggggaaattc tgacgccaga tataccccag gttccagctg gggtgagagc
     5701 agctcaggga gaataacaaa ttggcttgtt ctaaaaaacc ttacacctca gatcgatggc
     5761 tcaactctgc gcactctgtg catgcagcac ggcccgctga tcacattcca cctgaacctc
     5821 cctcacggaa atgctctggt ccgctacagt tcaaaagaag aggtagtgaa ggcacaaaag
     5881 tctctgcaca tgtgtgtact ggggaacact actattcttg ctgagtttgc cagtgaagag
     5941 gagatcagtc gtttctttgc acaaagccag tctctgaccc cttctcccgg ctggcagtct
     6001 ctcgggtcca gccagagccg gctgggctcc ctcgactgtt cccactcatt ctccagccgg
     6061 accgatctca atcactggaa tggtgctggg ctgtcgggaa ctaactgtgg agaccttcac
     6121 ggcacttcac tctgggggac cccgcattat tccacaagcc tgtggggtcc cccaagcagc
     6181 agcgaccccc gaggaattag cagcccatct cccattaacg cttttctttc tgttgaccac
     6241 ctgggtgggg gtggagagtc catgtaatct agagggcccg tttaaacccg ctgatcagcc
     6301 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     6361 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     6421 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     6481 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     6541 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     6601 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     6661 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     6721 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     6781 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     6841 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     6901 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     6961 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
     7021 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     7081 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     7141 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     7201 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     7261 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
     7321 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
     7381 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     7441 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     7501 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     7561 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
     7621 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     7681 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     7741 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     7801 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     7861 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     7921 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
     7981 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     8041 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     8101 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     8161 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     8221 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     8281 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     8341 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     8401 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     8461 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     8521 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     8581 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     8641 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     8701 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     8761 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     8821 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     8881 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     8941 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     9001 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     9061 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     9121 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     9181 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     9241 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     9301 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     9361 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     9421 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     9481 tggtagcggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     9541 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     9601 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     9661 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     9721 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     9781 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     9841 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     9901 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     9961 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    10021 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    10081 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    10141 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    10201 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    10261 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    10321 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    10381 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    10441 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    10501 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    10561 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    10621 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    10681 tccccgaaaa gtgccacctg acgtc
//
