LOCUS       sequence-56806-e       11182 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11182)
  AUTHORS   Nishi K, Nishi A, Nagasawa T, Ui-Tei K
  TITLE     Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated
            gene silencing in the nucleus.
  JOURNAL   RNA. 2013 Jan;19(1):17-35. doi: 10.1261/rna.034769.112. Epub 2012 
            Nov 13.
  PUBMED    23150874
REFERENCE   2  (bases 1 to 11182)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.034769.112"; journalName: "RNA"; date: "2013-01"; 
            volume: "19"; issue: "1"; pages: "17-35"
COMMENT     Sequence Label: pmyc-GFP-TNRC6C
FEATURES             Location/Qualifiers
     source          1..11182
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             910..939
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(949..951,952..954,955..1665)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(949..951,952..954,955..1665)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(994..1015)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1255..1274)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1602..1623
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3951..3968
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6776..6793)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6782..7006
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7052..7480
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7139..7158)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7349..7370
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7489..7509)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7494..7823
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7674..7809
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7736..7755
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7890..8684
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7944..7963)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8554..8573
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8858..8979
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8895..8914)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8949..8968
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9028..9044)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9028..9044)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9041..9063)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9052..9068
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9076..9082,9083..9100,9101..9106))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9121..9142
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9259..9276)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9430..10015)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9510..9529)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10186..10977,10978..11046))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10809..10828
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11047..11151)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 ccaccaatgg aacaaaaact catctcagaa gaggatctgt ccggcggcat ggtgagcaag
      961 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     1021 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     1081 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     1141 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     1201 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     1261 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1321 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1381 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     1441 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1501 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     1561 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1621 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagaagct tatggctaca
     1681 gggagtgccc agggcaactt cactggacat accaagaaga caaatggcaa taatggcacc
     1741 aatggcgcac tcgtccaaag cccttctaat cagagtgccc ttggagcagg gggagcgaac
     1801 agtaatggaa gtgcggccag agtgtggggt gtagccacag gctccagctc tggcctggct
     1861 cactgctctg tcagtggtgg ggatggaaaa atggacacta tgattggaga tgggagaagt
     1921 cagaattgct ggggtgcttc caactccaat gctggcatta atcttaacct taatcctaat
     1981 gccaacccag ctgcctggcc tgtacttgga catgaaggaa ccgtggcgac aggcaaccct
     2041 tccagtattt gcagtccagt cagtgccata ggtcaaaata tgggcaacca gaacgggaac
     2101 ccaacaggca ctttaggtgc ttggggaaac ttgctgccac aagagagcac agaaccacaa
     2161 acgtccactt ctcagaatgt gtctttcagc gcacaacctc agaaccttaa cactgatgga
     2221 ccaaataaca ctaaccccat gaactcttca cccaacccta tcaatgcaat gcagacaaat
     2281 ggactgccaa actggggcat ggctgttggt atgggggcca tcatcccgcc ccacctgcaa
     2341 ggccttcctg gtgctaatgg atcatcagtt tctcaagtca gtgggggcag tgctgaagga
     2401 ataagcaatt ctgtgtgggg actgtcccca ggtaaccctg ccacaggaaa tagcaattct
     2461 gggttcagtc aggggaatgg agacactgtg aactcagcat taagtgctaa acaaaatgga
     2521 tccagcagtg ctgtgcaaaa ggaaggaagt ggaggaaatg cttgggattc aggacctcct
     2581 gctggtcctg gaatactcgc ctggggaagg ggcagtggca acaatggcgt tggtaatatc
     2641 cattcaggag cttggggcca ccccagccga agcacctcta acggtgtgaa tggggaatgg
     2701 ggaaagcccc caaaccagca ttccaacagt gacatcaatg ggaaaggatc aacagggtgg
     2761 gagagtccta gtgtcaccag ccagaaccct accgtacagc ctggtggtga acacatgaac
     2821 tcctgggcca aagcggcatc ttctggaact acagcaagtg aaggaagtag tgatggttct
     2881 ggcaaccaca atgaaggaag cactgggagg gaaggaacgg gagaaggccg aaggcgagat
     2941 aaagggatta tagaccaagg gcacatccag ttgccaagga atgatcttga cccaagagtt
     3001 ctgtctaata ctggttgggg acagactcct gtaaagcaaa acactgcctg ggaatttgaa
     3061 gaatccccta ggtctgaaag gaaaaatgac aatgggacag aggcctgggg ttgtgcagct
     3121 actcaggctt caaactcagg ggggaagaac gatgggtcca tcatgaacag tacaaatacc
     3181 tcttcagtat ctgggtgggt caacgcgcca cctgccgctg tgccagcaaa cacaggttgg
     3241 ggagacagca acaacaaagc gccaagtggc ccgggggttt ggggggactc gataagctct
     3301 actgctgtta gtactgctgc tgctgccaag agtggccatg cttggagtgg ggccgcaaat
     3361 caggaggaca agtcacccac ctggggtgag cctccaaagc ccaaatccca acactgggga
     3421 gatggacaaa gatcaaatcc agcctggagt gcaggagggg gagattgggc agattcatcg
     3481 tctgtccttg gacacttggg ggatgggaaa aaaaatggat ctggatggga tgctgacagt
     3541 aataggtcag ggtctggttg gaatgacacc acgagatctg ggaacagtgg ctggggcaac
     3601 agcacaaata caaaggccaa tccaggtaca aactgggggg agactttaaa acctggcccc
     3661 caacagaact gggctagcaa accccaagac aacaatgtga gtaactgggg aggagctgct
     3721 tctgtgaaac agacaggaac agggtggatc ggggggccgg taccggtcaa acagaaggac
     3781 agcagtgaag caactggctg ggaagaaccc tctccaccgt ccattcgccg caaaatggaa
     3841 attgatgatg gtacctcagc ttggggggac ccaagcaact ataacaataa aactgtaaac
     3901 atgtgggata gaaacaaccc ggtcatccag agcagtacca cgaccaatac caccaccacc
     3961 accaccacta ccacgagcaa caccacacac agggtcgaga cgccgccccc gcaccaggcc
     4021 ggtactcagc tgaatcgatc accgttgctt ggtccaggta ggaaagtttc atcaggctgg
     4081 ggagaaatgc ctaatgttca ctcaaagact gaaaactctt ggggagaacc atcctcccct
     4141 tctaccctgg tggataatgg cacagcagca tgggggaagc cacccagcag tggcagcggg
     4201 tggggagatc accctgcaga gccgccggtg gcatttggaa gagctggcgc acctgttgct
     4261 gcctcagccc tgtgcaaacc agcttcaaaa tctatgcaag aaggctgggg cagtggtggg
     4321 gatgaaatga acctcagtac cagccagtgg gaggatgaag aaggggacgt gtggaataat
     4381 gctgcttccc aagaaagcac ctcctcctgc agctcctggg ggaacgcccc caaaaaagga
     4441 cttcaaaagg gcatgaagac gtctggcaag caggatgagg cctggatcat gagccggctg
     4501 atcaaacaac tcacagacat gggcttcccg agagagccag ctgaggaggc cttgaagagt
     4561 aacaatatga atcttgatca ggccatgagc gctctgctgg aaaagaaggt ggacgtggac
     4621 aagcgtgggc tgggagtgac cgaccataat ggaatggccg ccaagcccct cggctgccgc
     4681 ccgccaatct ccaaagagtc ttccgtggac cgccccacct ttcttgacaa ggatggcggc
     4741 ctcgtggaag agcccacgcc ttcaccgttc ttgccttccc caagcctgaa gctccccctt
     4801 tcacacagtg cactccccag tcaggccctg ggtgggattg cctccgggct gggcatgcaa
     4861 aacttgaatt cttctagaca gataccgagt ggcaatctgg gtatgtttgg caatagtgga
     4921 gcagcacaag ccaggaccat gcagcagccg ccacagccac cagtgcagcc tcttaactct
     4981 tcccagccca gtctccgtgc tcaagtgcct cagtttctat cccctcaggt tcaagcacag
     5041 cttttgcagt ttgcagcaaa aaacattggt ctcaaccctg cactattaac ctcgccaatt
     5101 aatcctcaac atatgacgat gttgaaccag ctctatcagc tgcagctggc ataccaacgt
     5161 ttacaaatcc agcagcagat gttacaggcc cagcgtaatg tgtccggatc catgagacaa
     5221 caggagcagc aagttgcgcg cacaatcact aatctgcagc agcagatcca gcagcaccag
     5281 cgccagctgg cccaggccct gctcgtgaag cagccaccac cgccaccgcc cccgccgcac
     5341 ctgtctctgc acccctctgc aggcaaatcg gccatggaca gcttcccctc gcacccacag
     5401 actcccggcc tacctgacct gcagaccaaa gagcagcagt cttcacccaa cacctttgct
     5461 ccttaccctc tcgctggact gaacccaaac atgaatgtca acagcatgga catgaccggt
     5521 ggcttgtcgg tgaaggaccc atcccagtcc cagtcacgcc tcccccagtg gacgcacccc
     5581 aactccatgg ataacttgcc cagtgccgct tcccccctgg agcagaaccc tagcaagcat
     5641 ggtgctatcc ctggaggtct aagcattggg cctccaggta agtcctccat tgatgactcc
     5701 tatggccggt acgatttaat ccagaacagt gagtcaccag ccagtcctcc cgtagctgtt
     5761 ccccatagct ggtcacgtgc caaatctgac agtgataaaa tctcaaatgg ctctagcatc
     5821 aactggcccc cagaattcca tccgggagtt ccatggaaag gactgcagaa tattgaccct
     5881 gagaatgacc ctgacgtcac tcctggcagt gtccccactg ggcctaccat caacaccacc
     5941 atccaggatg tcaaccgcta cctcctcaag agtggaggta aactgtcaga catcaaatcg
     6001 acgtggtcct ctggccctac ctcccacacg caagcctctc tgtctcatga actatggaag
     6061 gtgcccagaa acagtactgc acccacgagg ccacctccag ggttaaccaa tcccaagccc
     6121 tcctccacct ggggtgccag ccccctcggc tggaccagct cctactcctc gggttctgcc
     6181 tggagcaccg acacctcagg aagaaccagc agctggctcg ttcttcgaaa cctcactccc
     6241 cagattgatg gttctacact gcggacattg tgtttgcaac atgggcctct tatcacattc
     6301 cacctgaatc tgactcaagg caatgctgtg gtccggtaca gctccaagga ggaggctgcc
     6361 aaggcccaga agtctctgca catgtgcgtc ctgggaaaca ctaccatcct ggccgagttc
     6421 gctggtgaag aagaagtgaa tcgcttctta gcccaaggcc aggcgctgcc acccacttcc
     6481 agctggcagt ccagcagcgc gtccagccag ccgcggctca gcgcagcggg cagctcccac
     6541 ggcctggtac gcagcgacgc tggccactgg aacgccccgt gcctgggtgg caaggggagc
     6601 agtgagctgc tgtggggcgg ggtgccccag tactccagca gcctgtgggg cccgcccagc
     6661 gccgacgaca gcagggtgat aggcagcccc acgccgctaa ccaccctgct gcctggggac
     6721 ctgctcagcg gggagtccct gtagtctaga gggcccgttt aaacccgctg atcagcctcg
     6781 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     6841 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     6901 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     6961 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     7021 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     7081 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     7141 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     7201 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     7261 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     7321 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     7381 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     7441 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     7501 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     7561 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     7621 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     7681 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     7741 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     7801 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     7861 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     7921 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     7981 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     8041 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
     8101 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     8161 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     8221 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     8281 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     8341 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     8401 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     8461 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     8521 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     8581 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     8641 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     8701 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     8761 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     8821 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     8881 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     8941 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     9001 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     9061 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     9121 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     9181 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     9241 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     9301 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     9361 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     9421 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     9481 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     9541 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     9601 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     9661 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     9721 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     9781 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     9841 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     9901 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     9961 tagcggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
    10021 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
    10081 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
    10141 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
    10201 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
    10261 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
    10321 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
    10381 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
    10441 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
    10501 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    10561 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    10621 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    10681 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    10741 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    10801 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    10861 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    10921 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    10981 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    11041 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    11101 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    11161 ccgaaaagtg ccacctgacg tc
//
