LOCUS       sequence-56822-e       10499 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10499)
  AUTHORS   Nishi K, Nishi A, Nagasawa T, Ui-Tei K
  TITLE     Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated
            gene silencing in the nucleus.
  JOURNAL   RNA. 2013 Jan;19(1):17-35. doi: 10.1261/rna.034769.112. Epub 2012 
            Nov 13.
  PUBMED    23150874
REFERENCE   2  (bases 1 to 10499)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.034769.112"; journalName: "RNA"; date: "2013-01"; 
            volume: "19"; issue: "1"; pages: "17-35"
COMMENT     Sequence Label: pcDNA5/FRT/TO/FLAG-HA-SBP-TNRC6A-NES-mut
FEATURES             Location/Qualifiers
     source          1..10499
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        384..587
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     538..558
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    589..607
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    610..628
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     638..662
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      758..767
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             779..793
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             794..820
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             824..937
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     protein_bind    5167..5178
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     primer_bind     complement(6102..6118)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6103..6119)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(6174..6193)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6175..6193)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6220..6237)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6226..6450
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6583..6602)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    6734..6781
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6789..7811
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             6789..7811
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    7941..8062
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7978..7997)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8032..8051
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8111..8127)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8111..8127)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8124..8146)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8135..8151
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8159..8165,8166..8183,8184..8189))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8204..8225
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8342..8359)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8513..9101)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8593..8612)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9272..10063,10064..10132))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9895..9914
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10133..10237)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10312..10331)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc cctatcagtg
      601 atagagatct ccctatcagt gatagagatc gtcgacgagc tcgtttagtg aaccgtcaga
      661 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca
      721 gcctccggac tctagcgttt ccaccgcggt ggcggccgcc accatggatt acaaggatga
      781 cgacgacgat aagtacccat acgatgttcc agattacgct tctatggacg agaagaccac
      841 cggctggcgg ggcggccacg tggtggaggg cctggccggc gagctggagc agctgcgggc
      901 caggctggag caccaccctc agggccagcg ggagccctgc ccgggcggat ccatggatgc
      961 tgattctgcc tccagttctg aatcagagag aaacatcact atcatggctt cagggaacac
     1021 aggtggtgaa aaagatggcc ttcggaatag cactggactt ggttcccaaa acaagtttgt
     1081 agttggtagc agcagcaata atgtgggcca tggaagtagt actgggccat ggggtttttc
     1141 ccatggagcc ataataagca catgtcaggt ctctgtggat gctcctgaaa gcaaatctga
     1201 aagtagcaac aatagaatga atgcttgggg cactgtaagt tcttcatcaa atggagggtt
     1261 aaatccaagc actttgaatt cagctagcaa ccatggtgcc tggccagtat tagagaacaa
     1321 tggacttgcc ctaaaagggc ctgtagggag tggtagttct ggcattaata ttcagtgcag
     1381 tactataggc cagatgccta acaatcagag tattaactct aaagtgagtg gtggttctac
     1441 ccatggtacc tggggaagcc ttcaggaaac ttgtgaatct gaagtaagtg gtacacagaa
     1501 ggtttcattc agtggtcaac ctcaaaatat taccactgaa atgactggac caaataacac
     1561 tactaacttt atgacctcta gtttaccaaa ctccggttca gtgcagaata atgagctgcc
     1621 tagtagtaac acaggggcct ggcgtgtgag cacaatgaat catcctcaga tgcaggctcc
     1681 atcaggtatg aatggcactt ccctttctca ccttagcaat ggagagtcaa aaagtggagg
     1741 ctcttatggt actacatggg gtgcctatgg ttctaattac tctggagaca aatgttcagg
     1801 ccctaatggc caagctaatg gtgacactgt gaatgcaact ctaatgcagc ctggcgtaaa
     1861 tggtcctatg ggcactaact ttcaagttaa cacaaacaaa ggaggtggtg tgtgggaatc
     1921 tggtgcagca aactcccaga gtacatcatg gggaagtgga aatggcgcaa attctggagg
     1981 aagtcgaaga ggatggggaa cccctgcaca aaacactggc actaatttac ccagcgttga
     2041 gtggaacaaa ctgcctagca atcagcattc caatgatagt gcaaatggca atggtaagac
     2101 gtttacaaat ggatggaaat ctactgagga agaggatcag ggttctgcca catctcagac
     2161 aaatgagcaa agcagtgtgt gggccaaaac aggaggtaca gtggagagcg atggtagtac
     2221 agaaagcact ggacgccttg aggaaaaagg aactggggaa agtcagagta gagacagaag
     2281 aaaaattgat cagcacacat tactccaaag cattgtaaac agaactgact tagatccacg
     2341 tgtcctgtcc aactctggtt ggggacagac tcctattaag cagaatactg cctgggatac
     2401 agaaacatca cctagagggg aacgaaagac tgacaatggg acagaggcct ggggaagctc
     2461 tgcaacacag acttttaact caggggcatg tatagataag actagcccta atggtaatga
     2521 tacctcatct gtatcagggt ggggcgatcc caaacctgct ctgaggtggg gagattccaa
     2581 aggctcaaac tgccaggggg ggtgggaaga tgattctgct gctacaggaa tggtcaagag
     2641 caatcagtgg gggaattgca aagaggagaa ggctgcatgg aatgactcgc aaaagaataa
     2701 acagggatgg ggtgatggac aaaaatcaag ccaagggtgg tctgtttctg ccagtgataa
     2761 ctggggagaa acttcaagga ataaccattg gggtgaggcc aataagaaat ccagctcagg
     2821 aggtagtgac agtgacaggt ccgtttccgg ttggaacgaa cttggtaaaa ctagttcttt
     2881 cacttgggga aacaacataa atccaaataa ttcatcagga tgggatgaat cttctaaacc
     2941 tactccttcc cagggatggg gagaccctcc aaagtctaat cagtctctag gttggggaga
     3001 ttcgtcaaag ccagtcagct ctccagactg gaacaagcaa caagacattg ttggatcttg
     3061 gggaatccca ccagctacag gcaaacctcc tggtacaggc tggctggggg gacctatacc
     3121 agccccagca aaagaagaag aacccacagg ctgggaggaa ccatccccag aatctatacg
     3181 tcgcaaaatg gagattgatg atggaacttc agcttgggga gatccaagca aatacaacta
     3241 caaaaatgtg aacatgtgga acaaaaacgt cccaaatggc aacagccgtt cagaccagca
     3301 agcacaggta catcagctgc taacgcctgc aagtgccatc tcaaacaaag aggcaagcag
     3361 tggctctggc tggggtgagc cctgggggga gccttctact ccagccacaa ctgtggataa
     3421 tggtacttca gcatggggta agcccataga cagtggtccc agctgggggg aacccattgc
     3481 tgcggcatcc agcacatcca cgtggggctc cagctctgtt ggtccacaag cattaagcaa
     3541 atctgggcca aaatctatgc aagatggctg gtgtggtgat gatatgccat tgcctggaaa
     3601 tcgccccact ggctgggaag aggaagagga tgtggagatt ggaatgtgga atagtaattc
     3661 atctcaagag cttaactcat ctttaaattg gccaccatat acaaagaaaa tgtcatcgaa
     3721 gggtctgagt ggcaaaaaaa ggagaaggga aaggggaatg atgaaaggtg gaaacaaaca
     3781 agaagaagcg tggataaatc cagccgttaa acaggcttca aacgccagtg cttcgagaga
     3841 ctcaccagag gaaaatgtac aaagcaataa gatggacctt tctggaggaa tgttacaaga
     3901 caaacgaatg gagatagata aacatagcct aaatattggt gattacaatc gaacggtcgg
     3961 gaaaggccct ggttctcggc ctcagatttc caaagagtct tccatggagc gcaatcctta
     4021 ttttgataag gatggcattg tagcagatga atcccaaaac atgcagttta tgtccagtca
     4081 aagcatgaag cttccccctt caaatagtgc actacctaac caggcccttg gctccatagc
     4141 agggctgggt atgcaaaact tgaattctgt tagacagaat ggcaatccca gtatgtttgg
     4201 tgttggaaac acagcagcac aaccccgggg catgcagcag cctccagcac aacctcttag
     4261 ttcatctcag cctaatctcc gtgctcaagt gcctcctcca ttactctccc ctcaggttcc
     4321 agtttcattg ctgaagtatg caccaaacaa cggtggcctg aatccactct ttggccctca
     4381 acaggtagcc atgctgaacc agctatccca gctaaaccag ctttctcaga tctcccagtt
     4441 acagcgattg ttagcgcagc agcaaagggc gcagagtcag agaagcgtgc cttctgggaa
     4501 ccggccgcag caagaccagc agggtcgacc tcttagtgtg cagcagcaaa tgatgcaaca
     4561 atctcgtcaa cttgatccaa acctgttggt gaagcagcag actccaccat ctcagcagca
     4621 gccactccat cagccagcca tgaagtcttt ccttgacaat gtcatgcccc acactacacc
     4681 tgagctgcaa aaagggccat caccaataaa tgctttcagc aacttcccta taggcttgaa
     4741 ctcaaacttg aatgtaaata tggatatgaa cagtattaaa gagccacagt caagactaag
     4801 gaagtggacg acagtggaca gcatttctgt gaacacatct ttggatcaaa actccagcaa
     4861 acatggtgct atttcaagtg gtttcaggct ggaagagtct ccatttgttc cctatgactt
     4921 tatgaacagc agtacttcac cagccagtcc tccaggttca ataggagatg gctggccacg
     4981 tgccaaatcg cctaacggct ctagcagtgt taattggcca ccagaatttc gtcctggtga
     5041 gccatggaaa ggttatccaa acattgaccc tgaaactgac ccttacgtca ctcctggcag
     5101 tgtcataaac aatctttcaa ttaatactgt gcgggaagtt gaccacctca gggacaggaa
     5161 cagtgggtca tcctcatcct tgaacaccac gctgccttca actagtgcct ggtcatccat
     5221 tcgtgcctcc aactacaacg ttcccctcag cagtacagca caaagcactt cagccagaaa
     5281 tagtgattcc aaattgacat ggtctcctgg ttcagttaca aacacctctc tggctcatga
     5341 gctgtggaag gtccctttgc cacctaaaaa catcactgct ccgtcccgcc cacctccggg
     5401 actgactggt cagaagccac ccttgtctac gtgggataat tctccccttc gtataggtgg
     5461 aggatgggga aattctgacg ccagatatac cccaggttcc agctggggtg agagcagctc
     5521 agggagaata acaaattggc ttgttctaaa aaaccttaca cctcagatcg atggctcaac
     5581 tctgcgcact ctgtgcatgc agcacggccc gctgatcaca ttccacctga acctccctca
     5641 cggaaatgct ctggtccgct acagttcaaa agaagaggta gtgaaggcac aaaagtctct
     5701 gcacatgtgt gtactgggga acactactat tcttgctgag tttgccagtg aagaggagat
     5761 cagtcgtttc tttgcacaaa gccagtctct gaccccttct cccggctggc agtctctcgg
     5821 gtccagccag agccggctgg gctccctcga ctgttcccac tcattctcca gccggaccga
     5881 tctcaatcac tggaatggtg ctgggctgtc gggaactaac tgtggagacc ttcacggcac
     5941 ttcactctgg gggaccccgc attattccac aagcctgtgg ggtcccccaa gcagcagcga
     6001 cccccgagga attagcagcc catctcccat taacgctttt ctttctgttg accacctggg
     6061 tgggggtgga gagtccatgt aatctaggat atcaagctta tcgataccgt cgacctcgag
     6121 ggggggcccg gtaccttaat taattaaggt accaggtaag tgtacccaat tcgccctata
     6181 gtgagtcgta ttacaattca ctcgaaaccc gctgatcagc ctcgactgtg ccttctagtt
     6241 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     6301 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     6361 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     6421 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     6481 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     6541 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     6601 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     6661 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     6721 gttcacgtac ctagaagttc ctattccgaa gttcctattc tctagaaagt ataggaactt
     6781 ccttggccaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt
     6841 tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct
     6901 tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca
     6961 aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg
     7021 acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca
     7081 cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc gcggaggcca
     7141 tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc
     7201 aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg
     7261 tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg
     7321 atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt
     7381 tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg
     7441 aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt
     7501 tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat
     7561 cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg
     7621 ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat
     7681 ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg
     7741 atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg
     7801 caaaggaata gcacgtacta cgagatttcg attccaccgc cgccttctat gaaaggttgg
     7861 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     7921 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     7981 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     8041 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
     8101 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     8161 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     8221 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     8281 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     8341 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     8401 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     8461 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     8521 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     8581 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     8641 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     8701 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     8761 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     8821 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     8881 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     8941 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     9001 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     9061 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     9121 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     9181 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     9241 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     9301 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     9361 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     9421 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     9481 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     9541 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     9601 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     9661 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     9721 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     9781 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     9841 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     9901 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     9961 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    10021 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    10081 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    10141 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    10201 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    10261 ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg
    10321 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga
    10381 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa
    10441 gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgc
//
